... Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium Naoko Tanaka 1 , Vale ´ rie Dumay 1 , Qianning ... are the nonspecific acid phosphatase from Shigella flexneri (PhoN-Sf) and the enzyme from Salmonella enterica ser. typhimurium (PhoN-Se) [13,14]. On the basis of se...
Ngày tải lên: 24/03/2014, 03:21
... conditions. Conversely, acid phosphatase activity was measured in the presence of NaF and of those of inhibitors that alter amidolytic activity. Incubation with inhibitors was per- fromed at pH 9 and at pH ... amidolytic activity was obtained by showing that commercial PAP hydrolysed the Abz-ISYQSSSTEEQ- EDDnp substrate at neutral pH, as does the puri®ed enzyme. The ratio of...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... Chen 1 , Binbing Ling 1 , Ramaswami Sammynaiken 3 and Jian Yang 1, 1. Drug Design and Discovery Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, 110 Science Place, ... IIB of ovarian cancer has only one elderly patient (80 years and older), the RIZ1 mRNA expression increase may not be necessarily representative for the disease stage. An...
Ngày tải lên: 25/10/2012, 11:15
Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf
... intrinsic transcriptional activity. Fig. 2. The transcriptional activity of IRF-3 depends on synergy between two sets of residues and is entirely dependent on mammalian CBP. (A) Transcriptional activity in S2 cells of ... transcriptional activity of IRF-3 is dependent on simultaneous contact with both the N- and C-termini of CBP, and on the physical integrity of CBP....
Ngày tải lên: 17/03/2014, 10:20
Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding 071000, China. 3. Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military ... arthroplasty [30,31]: Currently, the efficacy of arthroplasty has been significantly im- proved. However, nowadays, a lot of younger adults develop ischemic femoral head n...
Ngày tải lên: 25/10/2012, 11:18
Báo cáo y học: "The Syndrome of Frontonasal Dysplasia, Callosal Agenesis, Basal Encephalocele, and Eye Anomalies – Phenotypic and Aetiologica"
... different pattern of cerebral and facial manifestations ranging from normality to typical HPE phenotype. Penetrance is relatively low. Patient 1 of the present study was previously reported as ... comprises of the syndrome of midline facial defects, callosal agenesis, basal encephaloceles, and eye anomalies. It has been described under different eponyms, most commonly the mo...
Ngày tải lên: 03/11/2012, 10:05
Báo cáo y học: "Different effect of exercise on left ventricular diastolic time and interventricular dyssynchrony in heart failure patients with and without left bundle branch block"
... exercise LV dyssynchrony may play a more important role in the pathophysiology of left ven- tricular remodelling than baseline LV dyssynchrony (23). Probably for that reason additional hemodynamic ... mechanical dyssynchrony. These findings may further explain why patients with normal QRS duration may benefit from cardiac re- synchronization whether or not they have mechanical dyssyn...
Ngày tải lên: 05/11/2012, 11:32
Báo cáo Y học: IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1 docx
... structural studies and a candidate for clinical use in diagnosis of cockroach allergy and develop- ment of new forms of immunotherapy. Keywords: allergen; hypersensitivity; IgE antibody; asthma; cockroach. Sensitization ... inoculate 500 mL of medium and grown for 7 days. Analysis of transformants for integration of Bla g 1 DNA in P. pastoris genome. Yeast genomic DNA was is...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells pot
... analyzed substrate affinity of CoOMT for tetrahydrocolumbamine using this 80% pure tetrahydrocolumbamine. To quantify the enzymatic activity of CoOMT, the transfer of the 3 H-labeled methyl group ... synthesized and purified from berberine by two-step reactions according to the method of Cava and Reed [20,21]. Construction and sequencing of cDNA library of C. japonica Poly...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Coordinated action of protein tyrosine phosphatases in insulin signal transduction potx
... SH2 domain of SHP-2 and phosphotyrosine 1146 of the activated insulin receptor [66]. Insulin also induces the formation of a complex of IRS-1 and SHP-2, requiring the tyrosines 1172 and 1222 of IRS-1 ... both necessary and sufficient for its targeting to the ER [81]. Cleavage of this segment appears to release the enzyme from the ER and increase its specific activity [...
Ngày tải lên: 17/03/2014, 23:20