Báo cáo Y học: Stabilization of a (ba)8-barrel protein by an engineered disulfide bridge potx
Báo cáo Y học: Stabilization of a (ba)8-barrel protein by an engineered disulfide bridge potx
... Stabilization of a (ba)
8
-barrel protein by an engineered disulfide
bridge
Andreas Ivens
1
, Olga Mayans
2
, Halina Szadkowski
3
, Catharina Ju¨ rgens
1
, Matthias Wilmanns
2
and Kasper ... spontaneous
formation of the disulfide bond by auto-oxidation. Chro-
matography, fi rst o n an anion exchange r esin, then on
hydroxylapatite and finally on a size-exclusion gel, r...
Báo cáo y học: " Implementation of a new emergency medical communication centre organization in Finland an evaluation, with performance indicators"
... data that could allow for a struc-
tured evaluation of the organizational changes. It is evi-
dent that a well-planned evaluation of changes in the
organizations, before they are actually made, ... emergency
medical call centre organization reform in Finland.
Material and methods
A retrospective observational study was conducted in
the EMCC in East and Central Uusimaa, an area of...
Báo cáo y học: "Gain of a 500-fold sensitivity on an intravital MR Contrast Agent based on an endohedral Gadolinium-Cluster-Fullerene-Conjugate: A new chance in cancer diagnostics"
... radiation therapy (IMRT) and the use
of heavy ions demands absolute reliability of new
diagnostics and treatment planning for prostate and
brain tumors. By the fact that the rare earth metals ...
pentaacetate-bis-(methylamide) (Omniscan®), or
1,4,7-tris-(carbonyl-methyl)-10-(2’-hydroxypropyl)-1,4
,7,10-tetraazacyclodecane (Prohance®).[12, 13]
Biochemical safety studies for adverse...
Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... US on patient data collected using the US manufactured de-
vice and analyzed using the US-based software and New York data analysis center from pa-
tients in the US, Germany, and Asia was completed. ... MCG data and angiographic data. Fi-
nally, microvascular disease, not associated with de-
finable epicardial vessel lesions on angiography, re-
sulting in myocardial ischemia can create a fa...
Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... Residues of
the catalytic triad are indicated by an asterisk.
Secondary structural elements based on known
crystal structures of PRK are indicated by h
(helix) and s (strand) and calcium binding
ligands ... 5¢-ATGTTAAA
GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and
5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG
CCG-3¢, cloned into the expression vector pBAD-TOPO,
transformed into the E.coliTop10 strain...
Tài liệu Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase potx
... Technology and Medicine, London SW7 2AZ, UK;
2
Laboratory of Molecular Entomology and Baculovirology, Riken, Wako, Japan
Sugar conjugation is a major pathway for the inactivation
and excretion of both ... UDP-glucosyltransferase gene, Bmugt1,and
analysis of the activity of the enzyme towards a range of
Correspondence to D. R. O’Reilly, Department of Biology, Imperial
College...
Báo cáo Y học: Characterization of a low redox potential laccase from the basidiomycete C30 pptx
... molecular mass standards; lane 2: 0.5 lgof
purified LAC1; lane 3: 0.5 lgofpurifiedLAC2;lane4:10lgoftotal
extra cellular proteins. (B) Native PAGE stained with p-phenylene
diamine; lane 1: total extra ... activity of
934 UÆmg
)1
, for a final yield of 50% (Table 1). The pure
LAC2 produced a single band both on a SDS/PAGE gel, at
a molecular mass of approximately 65 kDa (Fig. 1A) , a...
Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
... Materials
and methods) and the n assayed usin g the stand ard O
2
consumption
assay at 25 °C. Data are exp ressed as per cent of enzyme activity in the
standard assay; th e lines through t he data ... GO, DAAO and MSOX on various
substrates showed that GO and DAAO oxidize
D
-proline,
D
-alanine and
D
-2-aminobutyrate with similar relative
efficiencies. Analogously, GO and MSOX show a fairl...
Báo cáo Y học: Characterization of a partially folded intermediate of stem bromelain at low pH ppt
... Characterization of a partially folded intermediate of stem
bromelain at low pH
Soghra Khatun Haq, Sheeba Rasheedi and Rizwan Hasan Khan
Interdisciplinary Biotechnology Unit, Aligarh Muslim ... the
spectral characteristics displayed by stem bromelain are
similar to those observed in case of papain and proteinase W
namely, a bilobal structure with predominantly a and
Corresponden...
Báo cáo Y học: Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles A possible role in membrane secretion pptx
... in membrane secretion
Kenji Oshima
1
, Naohito Aoki
1
, Takeo Kato
2
, Ken Kitajima
1
and Tsukasa Matsuda
1
1
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan;
2
Food ... collected and concentrated by centri-
fugal filtration. Streptavidin-precipitates (lanes 1–5), the concentrated
media (lanes 6–10) and the total cell lysates (lanes 11–15) were analyzed
by S...