Báo cáo Y học: Stabilization of a (ba)8-barrel protein by an engineered disulfide bridge potx

... Stabilization of a (ba) 8 -barrel protein by an engineered disulfide bridge Andreas Ivens 1 , Olga Mayans 2 , Halina Szadkowski 3 , Catharina Ju¨ rgens 1 , Matthias Wilmanns 2 and Kasper ... spontaneous formation of the disulfide bond by auto-oxidation. Chro- matography, fi rst o n an anion exchange r esin, then on hydroxylapatite and finally on a size-exclusion gel, r...

Ngày tải lên: 24/03/2014, 03:21

... data that could allow for a struc- tured evaluation of the organizational changes. It is evi- dent that a well-planned evaluation of changes in the organizations, before they are actually made, ... emergency medical call centre organization reform in Finland. Material and methods A retrospective observational study was conducted in the EMCC in East and Central Uusimaa, an area of...

Ngày tải lên: 25/10/2012, 10:02

... radiation therapy (IMRT) and the use of heavy ions demands absolute reliability of new diagnostics and treatment planning for prostate and brain tumors. By the fact that the rare earth metals ... pentaacetate-bis-(methylamide) (Omniscan®), or 1,4,7-tris-(carbonyl-methyl)-10-(2’-hydroxypropyl)-1,4 ,7,10-tetraazacyclodecane (Prohance®).[12, 13] Biochemical safety studies for adverse...

Ngày tải lên: 26/10/2012, 09:07

... US on patient data collected using the US manufactured de- vice and analyzed using the US-based software and New York data analysis center from pa- tients in the US, Germany, and Asia was completed. ... MCG data and angiographic data. Fi- nally, microvascular disease, not associated with de- finable epicardial vessel lesions on angiography, re- sulting in myocardial ischemia can create a fa...

Ngày tải lên: 03/11/2012, 10:58

... Residues of the catalytic triad are indicated by an asterisk. Secondary structural elements based on known crystal structures of PRK are indicated by h (helix) and s (strand) and calcium binding ligands ... 5¢-ATGTTAAA GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and 5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG CCG-3¢, cloned into the expression vector pBAD-TOPO, transformed into the E.coliTop10 strain...

Ngày tải lên: 21/02/2014, 01:21

... Technology and Medicine, London SW7 2AZ, UK; 2 Laboratory of Molecular Entomology and Baculovirology, Riken, Wako, Japan Sugar conjugation is a major pathway for the inactivation and excretion of both ... UDP-glucosyltransferase gene, Bmugt1,and analysis of the activity of the enzyme towards a range of Correspondence to D. R. O’Reilly, Department of Biology, Imperial College...

Ngày tải lên: 22/02/2014, 04:20

... molecular mass standards; lane 2: 0.5 lgof purified LAC1; lane 3: 0.5 lgofpurifiedLAC2;lane4:10lgoftotal extra cellular proteins. (B) Native PAGE stained with p-phenylene diamine; lane 1: total extra ... activity of 934 UÆmg )1 , for a final yield of 50% (Table 1). The pure LAC2 produced a single band both on a SDS/PAGE gel, at a molecular mass of approximately 65 kDa (Fig. 1A) , a...

Ngày tải lên: 08/03/2014, 09:20

... Materials and methods) and the n assayed usin g the stand ard O 2 consumption assay at 25 °C. Data are exp ressed as per cent of enzyme activity in the standard assay; th e lines through t he data ... GO, DAAO and MSOX on various substrates showed that GO and DAAO oxidize D -proline, D -alanine and D -2-aminobutyrate with similar relative efficiencies. Analogously, GO and MSOX show a fairl...

Ngày tải lên: 08/03/2014, 16:20

... Characterization of a partially folded intermediate of stem bromelain at low pH Soghra Khatun Haq, Sheeba Rasheedi and Rizwan Hasan Khan Interdisciplinary Biotechnology Unit, Aligarh Muslim ... the spectral characteristics displayed by stem bromelain are similar to those observed in case of papain and proteinase W namely, a bilobal structure with predominantly a and Corresponden...

Ngày tải lên: 24/03/2014, 00:21

... in membrane secretion Kenji Oshima 1 , Naohito Aoki 1 , Takeo Kato 2 , Ken Kitajima 1 and Tsukasa Matsuda 1 1 Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan; 2 Food ... collected and concentrated by centri- fugal filtration. Streptavidin-precipitates (lanes 1–5), the concentrated media (lanes 6–10) and the total cell lysates (lanes 11–15) were analyzed by S...

Ngày tải lên: 24/03/2014, 03:21