... 288–300.21 Tsutsui N, Saido-Sakanaka H, Yang WJ, Jayasankar V,Jasmani S, Okuno A, Ohira T, Okumura T, Aida K &Wilder MN (2004) Molecular characterization of a cDNA encoding vitellogenin ... primers(forward, 5¢-TAATACGACTCACTATAGGTACTATGTATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTCACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢). For PCR, the reaction mix consisted of 1 · Taq buffer contain-ing 1.5 mm MgCl2, ... and 0.25 lLofTaq DNA polymerase(Life Technologies, Carlsbad, CA, USA). PCR conditionswere denaturation at 95 °C for 1 min, annealing at 55 °C for 1 min and extension at 72 ° C for 1.5 min for...