... determined at 2.4 A˚ from porcineheart by our group [20] and at 2.1 A˚ from chickenheart by Huang et al. [21]. It contains four nonidenti-cal subunits: the FAD-binding protein or flavoprotein(Fp, ... kDa, 622 amino acids), the iron–sulfur protein(Ip, 29 kDa, 252 amino acids), and two membrane-anchor proteins (CybL, 15 kDa, 140 amino acids, and CybS, 11 kDa, 103 amino acids) with a total ... contain-ing 1% SDS, which was used to run SDS ⁄ PAGE with agradient-separating gel (10–15%). The resulting gel wassoaked in western blot Tris ⁄ glycine transfer buffer and assembled into the...