Báo cáo Y học: Structural determination of lipid A of the lipopolysaccharide from Pseudomonas reactans A pathogen of cultivated mushrooms doc

... Structural determination of lipid A of the lipopolysaccharide from Pseudomonas reactans A pathogen of cultivated mushrooms Alba Silipo 1 , Rosa Lanzetta 1 , Domenico Garozzo 2 , Pietro Lo Cantore 3 , ... fatty acid; no information was available about the fatty acid distribution on the proximal GlcN. Analysis of intact lipid A and ammonium hydroxide tr...

Ngày tải lên: 24/03/2014, 00:21

... the crown gall disease on a wide range of dicotyledonous (broad-leaved) plants, and especially to the members of the rose family such as apple, pear and cherry; some strains can attack also almond ... permethylated lipopolysaccharide was recovered in the organic layer of the water/chloroform extraction and converted into its partially methylated alditol acetates [11], whic...

Ngày tải lên: 24/03/2014, 04:21

... the fatty acid on the acyoxyacyl group to be mainly 15, confirming the result of the compositional analysis. In the minor triacylated lipid A, the fragmentation patterns of the parent ion at m/z ... Structural study on lipid A and the O-specific polysaccharide of the lipopolysaccharide from a clinical isolate of Bacteroides vulgatus from a patient wi...

Ngày tải lên: 31/03/2014, 23:20

... Differential activity and structure of highly similar peroxidases. Spectroscopic, crystallographic, and enzymatic analyses of lignifying Arabidopsis thaliana peroxidase A2 and horseradish peroxidase A2 . ... peroxidase genes, encoding AtP1–24, AtP26, AtP31–33, AtPCa, AtPCb, AtPEa, AtPA2 and AtPN, as only these were available at the time. The majority were expressed from day 6–35 in...

Ngày tải lên: 21/02/2014, 01:21

... products of CR I–II. We thank Walter Chazin (Vanderbilt University, Nashville) for critical evaluation of the manuscript and Barbara Zarzycka (Warsaw) for her technical assistance. This work was supported ... and assignments at pH 6.7 and 7.7 are available from BMRB entry 4749). The AUTOASSIGN program [37] was used to obtain automatic assignments. By running the program with sever...

Ngày tải lên: 22/02/2014, 07:20

... Asn147; the Tyr146 !Ser/Asn147!Asp autolysis loop mutant had exactly the same molecular, enzymatic and autolytic properties as the Tyr146!His/Asn147 !Ser mutant. An Ala160!Leu variant of a rat ... fragment II in the degradation of the Tyr146!His/Asn147!Ser mutant (panel d) indicated a significant decrease in the rate of cleavage of the autolysis loop. At the same ti...

Ngày tải lên: 22/02/2014, 07:20

... which causes tularemia, a severe and often fatal disease of humans and other mammals [1]. The bacterium is an intracellular pathogen and therefore cell-mediated, rather than humoral, immunity is thought ... concentration. Lipid A was then purified by conventional silica gel chromatography. Com- parison of the 1 H-NMR spectra of the unfractionated lipid A and chromatogra...

Ngày tải lên: 08/03/2014, 09:20

... Thomas-Oates, J.E. & Brade, H. (1994) Preparation and structural analysis of oligosaccharide monophosphates obtained from the lipopolysaccharide of recombinant strains of Salmonella minnesota ... anion-exchange chro- matography (HPAEC) which had a molecular mass of 2486.59 Da as determined by electrospray ionization mass spectrometry. This was in accordance with the ca...

Ngày tải lên: 08/03/2014, 09:20

... contains several EF-hands such as Ca 2+ -dependent protein kinases from Arabidopsis thaliana, Zea mays, Glycine max, Dunaliella tertiolecta, Picea mari- ana, Solanum tuberosum, Plasmodium falciparum ... aberrant mobility, in the same way as other CaBPs [10,18–20]. The molecular mass as deter- mined by MALDI-TOF MS is 8672 Da. Analysis of the apo-calhepatin, at 7 l M monomer concentr...

Ngày tải lên: 08/03/2014, 22:20

... O-speci®c polysaccharides in a yield of 20% of the LPS mass. Rabbit antisera and serological assays Polyclonal O-antisera were obtained by immunization of rabbits with heat-inactivated bacteria of P. ... of the O-deacetylated polysaccharide compared to those at d 4.70 and 4.62 in the spectrum of the initial polysaccharide (a deshielding effect of the O-acetyl group)...

Ngày tải lên: 17/03/2014, 17:20