Báo cáo Y học: Puri®cation and characterization of the human

Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc

... N-terminal sequencing and amino-acid analysis. The sequence obtained, namely Lys- Ile-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the m ature maltose-binding protein. Binding of ... pressure and reduced exploratory activity indicating involvement of the receptor in a variety of physiological functions [6]. D irect structure determination of hA2aR...

Ngày tải lên : 24/03/2014, 00:21

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Báo cáo Y học: Puriﬁcation and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identiﬁcation as a product of liver origin doc

... (T in pig and human; M in rat), 614 (I in pig and human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human) , 991 (A in pig and human; S in rat), 1009 (M in pig and human; ... only in their carbohydrate moiety. Analysis of the carbohydrate structures revealed that the brain enzyme contains a glycostructure of th...

Ngày tải lên : 31/03/2014, 21:21

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Báo cáo Y học: Puriﬁcation and characterization of novel kininogens from spotted wolfﬁsh and Atlantic cod pdf

... an N-terminal heavy chain, the bradykinin moiety and a C-terminal light chain. The heavy chain and light chain are interlinked by disulﬁde bridges. Kininogens are further classiﬁed into high ... Most of the determined peptide sequences could be aligned with the heavy chain of human kininogen (Fig. 3), verifying the identity of these novel kininogens. The highly conserv...

Ngày tải lên : 08/03/2014, 23:20

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Báo cáo Y học: Puriﬁcation and characterization of three galactose speciﬁc lectins from Mulberry seeds (Morus sp.) ppt

... buffer containing 0.2 M galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the ... in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides and glycoproteins [...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Puriﬁcation and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx

... VanX- and VanY-type enzymes indicated that all of these enzymes contained the same Zn 2+ binding motif. VanX homologues present in the cyanobac- terium Synechocystis strain PCC6803, and the glycopeptide antibiotic ... Consequently the extent of interference of precursors terminating in D -Ser on the rate of hydrolysis of UDP-MurNAc-pentapeptide[Ala] was measured us...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Puriﬁcation and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt

... below. AP assays All assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is deﬁned as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... determined with the Coo- massie Blue G-250 dye-binding method [34] using bovine c)globulin as the protein standard. Peroxidase assay A chemiluminescence assay was em...

Ngày tải lên : 23/03/2014, 21:20

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Báo cáo Y học: Puriﬁcation and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx

... Ni 2+ ,Mg 2+ ,Zn 2+ ,Cu 2+ and Co 2+ .Partic- ularly, Cu 2+ and Zn 2+ potently inhibited chondroitin ABC lyase and chondroitin AC lyase. Heparin competitively inhibited only chondroitin AC lyase, not chondroitin ABC lyase. ... analyzed the internal sequences of a peptide obtained by digestion of each enzyme with trypsin (Table 3). The internal sequences of chondroitin ABC and...

Ngày tải lên : 31/03/2014, 23:20

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... digested with the same enzymes. The resulting plasmid (pET28yQ) was isolated from E. coli HB101 and the identity of the ybdQ insert was conﬁrmed by DNA sequencing. Finally, E. coli BL21(DE3)pLysS was transformed ... UspA domains in tandem [17]. The small members of this family are proteins of 142–144 amino-acids long; they are acidic and presumably located in the...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... of enzymes in the latex of Vas- concellea fruits, but in addition also results from the presence of other cysteine proteinases or isoforms. In contrast to the cysteine proteinases present in ... deg- radation, or in targeting or maturation of the enzyme, as was shown before in cathepsin C, which also belongs to the papain family of cysteine proteinases [27]....

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo Y học: Puriﬁcation and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx

... factors [3] and their biotechnological potential. Lipases have proven to be versatile enzymes in nonaqueous solvent systems in which they catalyze the synthesis of a variety of acylglycerols and specialized ... triacylglycerols comprised of both long and medium chain fatty acids has been investigated as a means of providing single substitutes for mixed acylglycerides in...

Ngày tải lên : 23/03/2014, 21:20

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