Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx
Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx
... Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates MicheÁle Brillard-Bourdet1, ... SBTI, E64 and EDTA did not signi®cantlyinhibit PAP amidolytic activity. However, NaF (10 mM),which strongly inhibits phosphatase activity at acidic pH,inhibited PAP amidolytic activity at neutral ... chromatography on phe-nyl-Sepharose. Fractions with enzymatic activity wereanalysed by MALDI TOF MS and reversed-phase HPLC on a C4 cartridge. A molecular mass of 47 798 Da wasdetermined by MS and...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... progression of cancer; and the second question is whether the PR domain alone possesses anticancer activity. In this study, we quan-titatively analyzed the mRNA expression level of RIZ1 over ... in many biological and physiological regulations. Re-cently, human tumor suppressor gene RIZ was shown to function in a Yin-Yang fashion [1, 2]. RIZ, located on the distal short arm of chromosome ... region of both RIZ1 and RIZ2, implicating the Yin-Yang fashion between RIZ1 and RIZ2 in cell reg-ulations [6, 16, 17]. Seventeen types of PRDMs have been identified in humans [15, 18]. Most of...
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Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf
... intrinsictranscriptional activity. Fig. 2. The transcriptional activity of IRF-3 depends on synergy betweentwo sets of residues and is entirely dependent on mammalian CBP. (A)Transcriptional activity in S2 ... immediate-early transcriptional response to infection and the secondaryresponse to cytokines [3–5]. For instance, IFN-b synthesis isactivated directly by virus infection in most cell types, or bylipopolysaccharide ... fusions. In conclusion, the results thatmutation of either set of Ser/Thr residues alone isaccompanied by a virus-dependent increase in activity, and that mutation of both sets is not, strongly suggest...
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Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium doc
... levels of acid phosphatase activity. On induction,the specific activity of acid phosphatase in both strains wasabout 40 UÆmg)1.During purification, the acid phosphatase activity alwayscochromatographed ... concentration was determined by using aprotein assay kit (Bio-Rad) with BSA as the standard.Enzymatic assay of phosphatase activity The phosphatase activity was measured by hydrolysis of 10 mMp-nitrophenyl ... 410 nm (absorption coeffi-cient 16.6 mM)1Æcm)1).Enzymatic assay of bromoperoxidase activity Assay of PhoN-Sf brominating activity. The brominating activity of the recombinant phosphatases...
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Báo cáo y học: "The Influence of Hyperbaric Oxygen Treatment on the Healing of Experimental Defects Filled with Different Bone Graft Substitutes"
... Turkey 4. Istanbul University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey. 5. Istanbul University, Faculty of Medicine, Department of Undersea and Hyperbaric ... Istanbul, Turkey 2. Istanbul University, Faculty of Medicine, Department of Oncologic Pathology, Istanbul, Turkey 3. Istanbul University, Faculty of Medicine, Department of Biochemistry, Istanbul, ... oxygen therapy: a study of bone mineral density and torsional strength on rabbits. J Trauma. 1998; 44: 676-681. 31. Nilsson LP, Granstrom G, Rockert HO. Effects of dextrans, heparin and hyperbaric...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... nude mice and the distribution and mi-gration of MSCs were dynamically monitored to evaluate the feasibility and safety of intravenous im-plantation of allogeneic MSCs in the treatment of femoral ... body temperature, mental condition, uri-nation, and defecation. Routine blood tests and tests of liver or renal functions showed normal. No acute or chronic toxicity and manifestations of ... osteonecrosis of femoral head. J Orthop Res. 2009 Apr;27(4):442-6. 6. Deng YB, Yuan QT, Liu XG, et al. Functional recovery after rhesus monkey spinal cord injury by transplantation of bone...
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Báo cáo y học: "The Syndrome of Frontonasal Dysplasia, Callosal Agenesis, Basal Encephalocele, and Eye Anomalies – Phenotypic and Aetiologica"
... Introdution Frontonasal dysplasia (FND) is the hallmark of several syndromes involving the frontonasal process that includes: isolated frontonasal dysplasia (MIM 136760 and 305645), acrofrontofacionasal ... dysostosis 1 (MIM 201180), acrofrontofacionasal dysostosis 2 (MIM 201181), frontofacionasal dysplasia (MIM 229400), oculoauriculofrontonasal syndrome (MIM 601452), among other related conditions ... hypothesis. In conclusion, the condition reported here is clinically homogeneous, and phenotypically it can be defined as frontonasal dysplasia, callosal agenesis, basal encephalocele and ocular...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... lenssorbitol dehydrogenase enzyme activity upon heating [46],while the N-terminal portion of a small heat shock/a-crystallin protein confers thermotolerance on cells [29] and prevents aggregation of stressed ... Jong, W.W. (1992) Expression and aggre-gation of recombinant aA-crystallin and its two domains. Bi ochim.Biophys. Acta 1130, 267–276.8. Augusteyn, R.C. (1998) a-Crystallin polymers and polymerization:the ... 937DISCUSSIONRestriction digestion, PCR amplification and sequencingconfirmed the identity of p26 cDNA fragments cloned inpET21(+) and pRSETC. The deduced amino -acid sequence fo r each construct...
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Báo cáo Y học: The effect of amino-acid substitutions I112P, D147E and K152N in CYP11B2 on the catalytic activities of the enzyme pdf
... asessential hypertension, cardiovascular or endocrine diseases,Fig.3.Assessmentof11b-h ydroxylase a ctivity and determination of 11b-hydroxylase capacity. (A)Assessmentof11b-hydroxylase activity of CYP11B2 ... KFig. 2. Enzymatic activities of aldosterone synthase and 1 1b-hydroxy-lase. (A) Enzymatic activities of aldosterone synthase and 11b-hydroxylase w ild-type enzymes and different CYP11B2 site-directedmutants ... Determination of 11b-hydroxylase capacity of CYP11B2 mutants in relation to the wild-type enzyme, whenincubated with 11-deoxycortisol. The 11b-hydroxylation of 11-deoxycortisol catalysed by the mutated...
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Báo cáo Y học: Stimulated biosynthesis of flavins in Photobacterium phosphoreum IFO 13896 and the presence of complete rib operons in two species of luminous bacteria Sabu Kasai and Takumi Sumimoto pot
... synthesis of flavins in P. phosphoreum (Eur. J. Biochem. 269) 5857synthase (ribB) is one of the LuxR- and acylhomoserinelactone-controlled nonlux genes, and also that the gene ismonocistronic and ... a member of the rib operon [29]. Thisposes the question of whether rib genes are organized inan operon and the genes for 3,4-dihydroxy-2-butanone4-phosphate synthase and GTP cyclohydrolase ... operon and stimulatedsynthesis of riboflavin does not occur.Correspondence to S. Kasai, Department of Bioapplied Chemistry,Faculty of Engineering, Osaka City University, Sugimoto 3-3-138,Sumiyoshi-ku,...
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