Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase pdf

... Open Access Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase Oscar N Ruiz * , Derry Alvarez, Gloriene Gonzalez-Ruiz and Cesar ... bioremediation efficiency of transgenic bacteria expressing metallothio- nien and polyphosphate kinase to understand which of these genes is best suited...

Ngày tải lên: 23/03/2014, 22:20

... analysed by means of sigmaplot 2001 (SPSS Science Software Gmbh, Erkrath, Germany). Data for the characterization of the kinetics of electrogenic BSP uptake fitted the Michaelis–Menten equation and ... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade. Acknowledgements Thanks are due to Prof G.L. Sottocasa and Dr Anto- nella Bandiera (Univ...

Ngày tải lên: 20/02/2014, 01:20

... Tropel and J. R. van der Meer 1766 FEBS Journal 272 (2005) 1756–1766 ª 2005 FEBS Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector ... Switzerland 2 Department of Fundamental Microbiology, University of Lausanne, Switzerland The metabolism of 2-hydroxybiphenyl (2-HBP) in Pseudomonas azelaica HBP1 is init...

Ngày tải lên: 07/03/2014, 17:20

... Linhardt 1 and Niels H. H. Heegaard 2 1 Division of Medicinal and Natural Products Chemistry, Department of Chemistry and Department of Chemical Engineering, University of Iowa, USA; 2 Department of ... circles) as a function of the total peptide concentration. A stock solution consisting of 125 l M scrambled peptide and 62 l M of each of the AP-1 and the mAP-1 pep...

Ngày tải lên: 31/03/2014, 23:20

... that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or turnover. A large number of APUM target candidates: ... wild-type and CLV-1 were synthesized and annealed. Each annealed pair of oligonucleotides possess a 32 bp sequence of a 3¢ UTR region of transcripts containing the APBE,...

Ngày tải lên: 18/02/2014, 06:20

... interacted Table 2. Sterol analysis of cells overexpressing STE20 and CLA4. Data are mean values with standard deviation from at least two independent experiments. lg of sterol per mg of protein pRS425 pRS425-STE20 ... Deletion of CLA4 results in higher amounts of SE. (A) Quan- tification of SE in cell polarity mutants. Cells of the indicated strains were grown to stationary ph...

Ngày tải lên: 18/02/2014, 13:20

... terminated by the addition of EDTA to a final concentration of 26 m M .After the addition of D 2 O to a final concentration of 10% (v/v), the samples were analysed by NMR spectroscopy. Assay of lactate ... accompanied by the appearance of three new 13 C-coupled signals at 143.4, 111.3 and 21.4 p.p.m. assigned as the carbon atoms 3, 4, and 5 of IPP, respectively (cf. Tabl...

Ngày tải lên: 19/02/2014, 13:20

... trained personnel and their replacement by untrained ones, inadequate supply of stains and other requirements, and quality of supplies particularly stains and slides. Some of these aspects ... to staff of general health services and periodic supervision followed by corrective actions, to ensure proper standards of work. In actual practice, the type of training...

Ngày tải lên: 06/03/2014, 04:20

... channels and designing appeals, implementing long term positioning strategies, and organisational and management issues (Bloom and Novelli, 1981). Lefebvre and Flora (1988) and Hastings and ... starts and finishes with research, and research is conducted throughout to inform the development of the strategy. A situational analysis of the internal and external...

Ngày tải lên: 06/03/2014, 21:20

... variants Evaluation of the hydrolysis of Ub-AMC and Ub-K- TAMRA by USP7-FL and its domain deletion Fig. 4. Enzymatic characterization of USP7. (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC ... proteolysis of USP7 variants in the presence and absence of ubiquitin. SDS ⁄ PAGE (4–20% gradient gels) showing the limited proteolysis of native USP7-FL and...

Ngày tải lên: 07/03/2014, 05:20