Báo cáo khoa học: Polyamines interact with DNA as molecular aggregates Luciano D’Agostino1 and Aldo Di Luccia doc
Báo cáo khoa học: Polyamines interact with DNA as molecular aggregates Luciano D’Agostino1 and Aldo Di Luccia doc
... single polyamines and digested by DNase I. (A) m: 1 kb DNA ladder
DNA; lanes A and B, DNA and DNA + DNase I, respectively; lane
C, DNA + 8000 NAP + Dnase I; lane D, DNA + 4800 NAP +
Dnase I; ... E, DNA + < 1000 NAP + DNase I. (B) Lanes A
and B, DNA and DNA + DNase I, respectively; lane C, DNA +
spermine + Dnase I; lane D, DNA + spermidine + DNase I; lane
E,...
Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc
... Cox6a and Cox7a after DDM solu-
bilization was poorly resolved using digitonin (Fig. 2B,
marked with an asterisk). However, Cox6a and Cox7a
as well as Cox6b assembled into holo-complex IV and
into ... subunits.
35
S-labeled
complex IV subunits were individually incu-
bated with isolated fibroblast mitochondria
for increasing times as indicated. Samples
were treated with prote...
Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc
... circadian clock.
In mammals, the circadian system is organized as a
hierarchical network of molecular clocks that operate
in different tissues, with the master clock residing in
the suprachiasmatic ... compilation ª 2009 FEBS
was exchanged for medium containing 50% horse serum.
After 2 h, this medium was replaced with serum-free
medium and the cells were harvested at the indicated t...
Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... fluorescence obtained with 200 nm
RNaseAlert and DNaseAlert was measured by adding 5 lL
RNase A (0.01 UÆmL
)1
) to wells with RNaseAlert substrate
after the initial measurements and 2 lL undiluted VcEndA
to ... cleave plasmid DNA,
dsDNA and ssDNA as shown in Fig. 8.
To test the RNA specificity of the enzymes, we used
the RNaseAlert assay and compared the results with
those obt...
Tài liệu Báo cáo khoa học: "Finding Hedges by Chasing Weasels: Hedge Detection Using Wikipedia Tags and Shallow Linguistic Features" doc
... weasel contexts and the corpus in
general and b) on the average distance the word
has to a weasel tag, if found in a weasel context.
We assume that a word is an indicator for a weasel
if it occurs ... heads
found in sentence S.
6 Results and Discussion
Both, the classifier based on words preceding
weasel (wpw) and the one based on added syntac-
tic patterns (asp) perform comparably wel...
Tài liệu Báo cáo khoa học: "Automatic Evaluation of Machine Translation Quality Using Longest Common Subsequence and Skip-Bigram Statistics" doc
... 0.83
With Case Information (Case) Lower Case (NoCase) Lower Case & Stemmed (Stem)
With Case Information (Case) Lower Case (NoCase) Lower Case & Stemmed (Stem)
Table 1. Pearson’s ρ and ... consider B
LEU with unigram and bi-
gram, i.e. N=2, for the purpose of explanation and
call this B
LEU-2. Using S1 as the reference and S2
and S3 as the candidate translations, S...
Báo cáo khoa học: cAMP response element-binding protein (CREB) is imported into mitochondria and promotes protein synthesis docx
... subjects. The CREB
cDNA was cloned in the pGEM vector with the T7
promoter. Plasmid construction was confirmed by DNA
sequencing. In vitro transcription ⁄ translation of CREB
cDNA was performed in ... Biotech,
Madison, WI, USA) as reported by De Rasmo et al. [24].
One microgram of CREB construct was added to 50 lLof
Promega standard mixture, containing T7 RNA polymerase
and a standard...
Báo cáo khoa học: The hinge region operates as a stability switch in cGMP-dependent protein kinase Ia doc
... Their
molecular masses could be measured with an accuracy
of a few Daltons, as depicted in Table 1. For all three
proteins, the expected theoretical masses matched to
the measured masses, assuming, ... high affinity site and the succeeding low affinity
site display slow and fast cGMP-exchange characteris-
tics and affinity constants of 17 and 100–150 nm,
respectively [5,15]. Bindin...
Báo cáo khoa học: "Learning How to Conjugate the Romanian Verb. Rules for Regular and Partially Irregular Verbs" docx
... language acquisi-
tion point of view, dividing the 1st and 4th tradi-
tional classes into 3 and respectively 5 subclasses,
each with a different conjugational pattern, and
offering rules for alternations ... counts), and the op-
tional appending of a terminator character. The
terminator character allows the classifier to iden-
tify and assign a different weight to the n-grams
that...
Báo cáo khoa học: "Automatic Detection of Syllable Boundaries Combining the Advantages of Treebank and Bracketed Corpora Training" docx
... on-
set, nucleus, and coda than a syllable comprising
the onset and nucleus; the least probable struc-
ture are syllables with an empty onset, and syl-
lables with empty onset and empty coda. Rules
(1.8)-(1.10) ... training phase
bank and bracketed corpora training. In section
4 we describe the grammars and experiments for
German data. Section 5 is dedicated to evaluation
and...