Báo cáo khoa học: A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity pptx

... binding protein homologue from soybean exhibits GTP -binding activity that functions independently of sucrose transport activity Carlos P. Pirovani 1 , Joci Neuby A. Mace ˆ do 2 , Luı ´ s Anto ˆ nio ... discussed. Keywords: sucrose transporter; soybean; yeast complemen- tation assay; Glycine max. In many higher plants, sucrose is the predominant form of ph...

Ngày tải lên: 23/03/2014, 21:21

... DNA was extracted from cells that were not irradiated and from cells immediately after UV irradiation, and 7 and 20 h after irradiation with a dose of 12 JÆm )2 . One microgram of DNA was loaded ... Gam- per AM, Kundu TK, Chait BT & Roeder RG (2001) Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage...

Ngày tải lên: 07/03/2014, 00:20

... of rat polypyrimidine tract binding protein. J Biochem 128, 811–821. 18 Yamamoto H, Tsukahara K, Kanaoka Y, Jinno S & Okayama H (1999) Isolation of a mammalian homo- logue of a fission yeast ... 1283–1297. 24 Sakaki-Yumoto M, Kobayashi C, Sato A, Fujimura S, Matsumoto Y, Takasato M, Kodama T, Aburatani H, Asashima M, Yoshida N et al. (2006) The murine homolog of SALL4, a...

Ngày tải lên: 07/03/2014, 00:20

... D-Ala-(Gly) 2 (D-Ala) 2 , D-Ala-L-Ala (D-Ala) 3 (D-Ala) 4 , L-Ala-Gly, L-Ala-(Gly) 2 (L-Ala) 2 , L-Ala-D-Ala, L-Ala-D-Ala-L-Ala, DL-Ala-DL-Asn, DL-Ala-DL-Ile, DL-Ala-DL-Leu, DL-Ala-DL-Met, DL-Ala- DL-Phe, DL-Ala-DL-Ser, ... 5¢-CACTTG AAGCTTTAAGGAGGA AtagACCATGCGTATCCGTGAGCTTGGCATCACC-3¢; antisen se primer, 5¢-ACGCAA TCTAGAGTCAGCCCTCA GGGGGCTTTCG-3¢. The amplified PCR product was digested with...

Ngày tải lên: 07/03/2014, 21:20

... 200 wt 1A 2A 3A 4A 5A N10 M10 C10 LVCEETANYLVIWCIDLEVE AAAAETANYLVIWCIDLEVE LVCEAAAAYLVIWCIDLEVE LVCEETANAAAAWCIDLEVE LVCEETANYLVIAAAALEVE LVCEETANYLVIWCIDAAAA LVCEETANYL TANYLVIWCI VIWCIDLEVE Fig. 5. Five residues of Cdc37 are essential for the binding ... α-Myc whole FLAG-GST IP: α-FLAG B 1A 2A 3A 4A 5Awt 1A 2A 3A 4A 5Awt whole IP: α-FLAG N10 M10 C10wt C α-FLAG WB: α-Myc...

Ngày tải lên: 07/03/2014, 21:20

... fusion protein, the cDNA encoding p16 was obtained from the psp54 (28) cDNA. The oligonucleotides used as primers were: 5¢-CCCCTCGA GATGTCTAGACAAAAAAAGAGTAG-3¢ and 5¢-CCC CTCGAGTCACACAACAGCACGAC-3¢.ThePCR product ... acetylated, while H 2A contains a mixture of acetylated and nonacet- ylated isoforms and H2B was not acetylated at all. The acetylation and AUT/PAGE analysis of histone...

Ngày tải lên: 08/03/2014, 10:20

... 5¢-TCCTCCACTATAAAAGGAAATG-3¢ and DELa forward 5¢-TTATAGTGGAGGAAAATAAGAC-3¢; DELb reverse 5¢-AACAGTCCATTTTAGTCCTT-3¢ and DELb forward 5¢-AAAATGGACTGTTCTTTCTT-3¢; DELc reverse 5¢-TACCTAGAAGAAAGAACAGT-3¢ ... several in-house antibodies against PTB, hnRNP A1 ⁄ A2 ⁄ C and H proteins and commercial antibodies against ASF ⁄ SF2 (Zymed, Carlsbad, CA, USA), SC35 (Sigma) and SRp55 (1H4 antibody, Zyme...

Ngày tải lên: 16/03/2014, 04:20

... Sharma 1 and Jonathan M. Wright 1 1 Department of Biology, and 2 Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada We have determined the nucleotide sequence of a ... (MouseCRBPIII; AA466092), rat CRBPI (RatCRBPI; P02696), rat CRABPII (RatCRABPII; P51673), human CRABPI (HumanCRABPI; P29762), zebrafish brain FABP (ZbfBFABP; Af237712), and zebrafish intestinal...

Ngày tải lên: 17/03/2014, 11:20

... from Methanococcus jannaschii (accession no.: AAB98358) are shown. Amino acids that were conserved among at least six of the seven homologues are shaded in gray. DNA -binding surface of Arabidopsis ... structure of Top 6A (1D3Y) was obtained from the Protein Data Bank and was employed as the basal structure. A molecular diagram was generated using molfeat, version 2.2.1.8 (F...

Ngày tải lên: 29/03/2014, 09:20

... 5¢-CGGAACCAAAACATGC TAACATTTTC[FAM]G-3¢ (Invitrogen, Carlsbad, CA, USA) and the reverse primer 5¢-CGTTACAGGCA- ACTTGTTTCTCA-3¢ were used for detection of GmPDIM mRNA. ER fractionation Slices of ... Upregula- tion of mRNA of GmPDIM was detected by DNA array analysis with a genechip (Affymetrix, Santa Clara, CA, USA) designed from soybean expression sequence tags (data not shown)....

Ngày tải lên: 30/03/2014, 04:20