Báo cáo khoa học: Leishmania donovani phosphofructokinase Gene characterization, biochemical properties and structure-modelling studies doc

... characterization of the gene encoding Leishmania donovani phosphofructokinase (PFK) and the biochemical properties of the expressed enzyme are reported. L. donovani has a single PFK gene copy per haploid ... both detailed and schematic plots of protein structures. J. Appl. Cryst. 24, 946–950. Ó FEBS 2002 Leishmania donovani phosphofructokinase (Eur. J. Biochem. 269)...

Ngày tải lên: 23/03/2014, 21:21

... Extensive and diver- gent circadian gene expression in liver and heart. Nature 417, 78–83. 16 Rudic RD, McNamara P, Curtis AM, Boston RC, Panda S, Hogenesch JB & Fitzgerald GA (2004) BMAL1 and ... that gene expression follows the metabolic rhythm [21,40]. Almost all genes are cyclically transcribed and can be clustered in three groups: 650 are expressed in the oxidative phase,...

Ngày tải lên: 30/03/2014, 04:20

... et al. Gene fusion and Leishmania topoisomerase I FEBS Journal 274 (2007) 150–163 ª 2006 The Authors Journal compilation ª 2006 FEBS 163 Leishmania donovani bisubunit topoisomerase I gene fusion ... and steady-state kinetic analysis of the DNA strand cleavage and liga- tion reactions. Biochemistry 33, 327–339. 17 Carey J (1991) Gel retardation. Methods Enzymol 208, 103–117. G...

Ngày tải lên: 30/03/2014, 10:20

... cloning, expression and functional folding of MAT from the etiological agent of visceral leishmaniasis, Leishmania donovani, as well as the relevance of cysteine residues to both the AdoMet synthesis and the ... mutagenesis The full-length MAT2 gene was amplified by PCR using L. donovani genomic DNA as template. The sense and antisense primers were: 5¢-CGG GAT CCA TGT CTG TCC ACA...

Ngày tải lên: 08/03/2014, 08:20

... system to jointly identify definitions and extract hypernyms; 3. The generality of the method, which applies to generic Web documents in any domain and style, and needs no parameter tuning; 1325 ... calculated on the generalized sentences s  k of s k and s  j of s j as fol- lows: S a,b =  1 if ω k a = ω j b 0 otherwise where ω k a and ω j b are the a-th and b-th word classes of...

Ngày tải lên: 20/02/2014, 04:20

... been developed to be general purpose text annotation systems, e.g., MMAX-2 (Müller and Strube 2006), GATE (Cunningham et al 2002), WordFreak (Morton and LaCivita 2003) and Knowtator (Ogren ... creating and editing the coding scheme (the tag set). UAM CorpusTool uses a hie- rarchally organised tag scheme, allowing cross- classification and multiple inheritance (both dis- juncti...

Ngày tải lên: 20/02/2014, 09:20

... into their stem and suffix forms is beneficial when the split is performed using a morphologi- cal analyzer and (ii) allowing the model to choose stem and suffix dependencies separately and flex- ibly ... also experiment with words split into their stem and suffix forms, and define stem- suffix FlexGrams where one set of offsets is ap- plied to stems and another to suffixes. We evaluate...

Ngày tải lên: 20/02/2014, 09:20

... mutations in loops 2 and 4 and their effects on cis-cleavage in vitro. Biochemistry 36, 3930–3940. 24 Siwkowski A, DeYoung MB, Anderson P & Hampel A (1997) Mutagenesis and modeling of the ... helix–loop–helix domains, A and B (Fig. 1). The hairpin ribozyme can form an extended conformation (undocked state) or a bent con- formation (docked state). In the docked state, loops A and...

Ngày tải lên: 07/03/2014, 00:20

... ambiguity in speech and other NLP tasks, outperforming generative models. Discrimi- native training has been used mainly for translation model combination (Och and Ney, 2002) and with the exception ... al., 1998; Yamada and Knight, 2001; Chiang, 2005) and the string transduction models (Kanthak et al., 2005). In this paper, we present an alternate approach to lexical selection and...

Ngày tải lên: 17/03/2014, 04:20

... lachrymal and lingual salivary glands, and has been found to be expressed by several other secretory tissues such as prostate, mucosal glands of the tracheobronchi- al tree, nasal mucosa and sweat glands ... giving K d values of 1.5 and 0.5 lm, and saturation levels of 0.9 and 1.83 for porcine and bovine OBP, respectively were in agreement with functional preparations of native...

Ngày tải lên: 23/03/2014, 10:20