Báo cáo khoa học: Thermodynamic stability of porcine b-lactoglobulin A structural relevance docx

... Primary data processing was carried out using the NAIRTA software (Institute of Biochemical Physics, Moscow). The denaturation enthalpy was determined as the area of the heat capacity peak over a ... single endothermic heat capacity peak is observed within the whole pH range studied (a broad marginal peak of heat capacity at low temperatures seen at pH 10 was attributed to instabilit...

Ngày tải lên: 23/03/2014, 21:21

... S, Fujiwara S, Hashimoto H, Kai Y, Takagi M & Imanaka T (2001) Comparative analy- ses of the conformational stability of a hyperthermo- philic protein and its mesophilic counterpart. Eur J ... Thermotoga maritima. Biochemistry 38, 9169– 9178. 53 Ogasahara K, Nakamura M, Nakura S, Tsunasawa S, Kato I, Yoshimoto T & Yutani K (1998) The unusually slow unfolding rate causes the hi...

Ngày tải lên: 07/03/2014, 05:20

... Thermotoga maritima (HUTmar) has recently been purified and characterized Correspondence to P. L. Mateo, Department of Physical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain. Fax: ... heat effects of ionization are generally small and are also well compensated by the heat of proton transfer between the protein and the buffer, it is reasonable to assume t...

Ngày tải lên: 23/03/2014, 12:20

... Empirical equations have been derived that allow comparison of the experimental thermodynamic parameters and structure-based calculated parameters based on sur- face area parameterization. This approach ... DASA polar are the changes in ASA of the apolar and polar residues, respectively [39]. The structure-based calculations provide a DC p of )407 calÆmol )1 ÆK )1 and DASA apolar an...

Ngày tải lên: 19/02/2014, 05:20

... Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin Nabil A. M. Sultan, Bhaskar G. Maiya* and Musti J. Swamy School of Chemistry, University of Hyderabad, ... recorded at 25 °ConaJascoJ-810 spectropolarimeter (Jasco I nternational C o., L td, To kyo, Japan) available at the Central Instrumentation Labo ratory, University of Hyderabad. Spectra we...

Ngày tải lên: 19/02/2014, 16:20

... LEKL GAP JunW AS AAELEER VKTLKAE IYELQSE ANMLREQ IAQL GAP JunW CANDI AS AAELEER AKTLKAE IYELRSK ANMLREH IAQL GAP FosW Core AS IDELQAE VEQLEER NYALRKE VEDLQKQ AEKL GAP cJun(R) AS IARLRER VKTLRAR ... the bZIP domains of Jun and Fos. Our ITC data on the stability of the cJun–cFos interaction correlate well with thermal melting data (see Table 2 and [11]), chemical denaturation data [12] an...

Ngày tải lên: 06/03/2014, 01:20

... 520–523. 2 Kawada N, Kristensen DB, Asahina K, Nakatani K, Minamiyama Y, Seki S & Yoshizato K (2001) Charac- terization of a stellate cell activation-associated protein (STAP) with peroxidase activity ... unfolded [22,26]. Clearly, it is of interest to take advantage of the wealth of structural information available for Mb and apoMb for the comparative study of the molecular...

Ngày tải lên: 07/03/2014, 02:20

... Cervellati, Franco Dallocchio and Stefania Hanau Dipartimento di Biochimica e Biologia Molecolare, Universita ` di Ferrara, Italy Drugs designed to combat African trypanosomiasis are often based ... that of NADP. Indeed, aPyADP has been used as an analogue of the oxidized coenzyme in 6PGDH from Candida utilis [23]. The anomalous behaviour of aPyADP could result from the lack of the ca...

Ngày tải lên: 07/03/2014, 05:20

... 17b-dehydrogenase: structural and cata- lytic changes during urea-denaturation. Biochem Biophys Acta 1297, 219–227. 30 Nakanishi M, Kakumoto M, Matsuura K, Deyashiki Y, Tanaka N, Nonaka T, Mitsui Y & ... is characterized by negative CD bands near 208 and 222 nm and a positive band at 193 nm, which is typ- ical of an a- helical structure [20]. Based on contin analysis [21], at 25 °C...

Ngày tải lên: 16/03/2014, 13:20

... relaxation time of 100 fs. The volume of the box fluctuated by about 250 nm 3 , with a standard deviation of less than 0.0025 of its value. All structural analyses, in particular rmsds, sec- ondary ... Nakatani K, Minamiyama Y, Seki S & Yoshizato K (2001) Charac- terization of a stellate cell activation-associated protein (STAP) with peroxidase activity found in rat hepatic...

Ngày tải lên: 30/03/2014, 01:20