Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana doc

... Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana Steffen Osswald, 1 Harald Wajant 2 and Franz Effenberger 1 1 Institut fu È r Organische Chemie, and 2 Institut ... concen- tration of 0.5 m M for in duction of AtNIT1 expression. After an additional 6 h, cells were harvested. Preparation of the crude extract and puri®c...

Ngày tải lên: 23/03/2014, 21:21

... J., Anderson, L. & Eidne, K.A. (1993) Functional expression and molecular characterization of the thyrotrophin-releasing hormone receptor from the rat anterior pituitary gland. J. Mol. Endocrinol. ... consists of a large, rapid and transient response that is typical of Ca 2+ -dependent Cl – channels activated after stimulation of PLC and the subsequent InsP 3 -dependent...

Ngày tải lên: 21/02/2014, 03:20

... JHEH and JH-specific esterase. JHEH was purified from Manduca sexta [24], and the JHEH gene was isolated from M. sexta [25], Tricoplusia ni [26] and Ctenocephalides felis [27]. The activity and ... 12% (w/v) gel. A distinct band of 43 kDa was observed in all four samples, and no band of this size was seen in the control sample taken from cells infected with nonrecombinant ba...

Ngày tải lên: 07/03/2014, 21:20

... C-terminal domain of EstD (amino acid residues 150–412). The schematic structural model consists of six a-helixes and has one central b-sheet made up of six b-strand-strands (Fig. 3A). The first and second ... both sides of Ser243. The alcohol side of the substrate is in a groove pointed towards the entrance of the pocket and extends approximately 10 A ˚ from Ser243. The ac...

Ngày tải lên: 23/03/2014, 09:20

... family members of the same plant; and (b) the absence of one intron in the A. thaliana genes AtPaspA2 and At- PaspA3 [29]. Cardosins and cyprosins share a similar structural domain organization and display ... (1994) Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-spe...

Ngày tải lên: 30/03/2014, 09:20

... the active site gorges of TcAChE and HuAChE is evidence for the importance of water in the structure of AChE. The structure and number of water molecules in the gorge and bound to the enzyme ... enhancement of ANS binding from 125 MPa and E202Q from 200 MPa compared with wild- type enzyme. This suggests that pressure denaturation (i.e. appearance of newly solvent-expos...

Ngày tải lên: 08/03/2014, 10:20

... defensins and cathelicidins [13] and magainins from the skin of frogs [14]. There is a limited knowledge about the defence mechanisms of the epidermal mucus of cod. However, both consti- tutive and ... Institute of Iceland and 50 specimens were caught randomly. After killing the fish by a concussion of the brain by striking of the cranium 2 , mucus samples were collected...

Ngày tải lên: 23/03/2014, 15:21

... units from the nonreducing end of polygalacturonate. Detailed characterization of the enzyme showed that PelB is highly thermo-active and thermostable, with a melting temperature of 105 °C and ... exo- polygalacturonases and rhamnogalacturonases [3,4]. Although a handful of endopolygalacturonases, gener- ally of fungal origin [5–10], and a single rhamnogalac- turonase [1...

Ngày tải lên: 20/02/2014, 03:20

... 3. Overproduction and purification of recombinant AraL-His 6 . (A) Analysis of the soluble (S) and insoluble (P) protein fraction (20 lg of total protein) of induced cultures of E. coli Bl21(DE3) pLysS ... set of primers: ARA358 and ARA514, and ARA515 and ARA516 (Table 1). The resulting fragment of 216 bp, comprising the arabinose promoter region (Para) from )81 to...

Ngày tải lên: 14/03/2014, 23:20

... Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, University of Wisconsin–Madison, USA Introduction Upon ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and c...

Ngày tải lên: 14/02/2014, 22:20