Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt

... Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato ( Lycopersicon esculentum ) cell cultures Gale G. Bozzo 1 , Kashchandra G. ... growth and secreted AP activity of tomato suspension cells Tomato cells cultured for 8 days in the absence of exogen- ous P i had only approximately 40% of the...

Ngày tải lên: 23/03/2014, 21:20

... Kalkkinen 1 1 Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland, 2 Norwegian College of Fishery Science, University of Tromsø, Norway, 3 Institute of Medical Biology, University ... biantennary and triantennary N-glycans that are terminated by a2,3-linked sialic acids, a high number of which are O-acetylated. About 1/3 of the glycans car...

Ngày tải lên: 08/03/2014, 23:20

... in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides and glycoproteins [1,2]. Plant lectins isolated from a wide variety of plants ... hemagglutinating activity, respectively. Although the yield of these proteins was found to be decreased by the purification steps and over 96% of protein...

Ngày tải lên: 18/03/2014, 01:20

... mutagenesis [20], and later confirmed by analysis of the crystal structure [19]. Comparison of the active site of VanXY C with VanX- and VanY-type enzymes indicated that all of these enzymes contained ... mass of 44 kDa. This method yielded 3–4 mg of His 6 –VanXY C from a culture volume of 1 L for enzyme characterization studies and was also used for purification of...

Ngày tải lên: 18/03/2014, 01:20

... determination of hA2aR by X-ray or electron crystallography, or infor- mation on the bound ligand conformation obtained by NMR spectroscopy, would assist in the design of subtype speci®c compounds and ... Biosystems). Protein assay and amino -acid analysis Protein concentrations were determined by the Amido black assay [24] using BSA as a standard. For the puri®ed receptor, amino -ac...

Ngày tải lên: 24/03/2014, 00:21

... (1990) Regulation and cellular localization of the membrane-bound thyrotropin-releasing h ormone-degrading enzyme in primary cultures of neur onal, glial and aden ohypophyseal cells. Endocrinology 127 , ... TRH-DE from rat and pig brain [28]. For the purification of the serum TRH- DE fractionation by poly(ethylene glycol) precipitation was used as the first step not only to pa...

Ngày tải lên: 31/03/2014, 21:21

... to homogeneity with yield of 2.37% by a series of CM-Sepha- dex C-50 column chromatography (Fig. 1), hydroxyapatite column and Sephacryl S-300 gel filtration chromatography, and its final specific activity ... each enzyme with trypsin (Table 3). The internal sequences of chondroitin ABC and AC lyase show significantly greater homology of 59 and 80% to Flavobacterial chondroitin AC...

Ngày tải lên: 31/03/2014, 23:20

... dhkim@khu.ac.kr Abbreviations: CM, carboxymethyl; DEAE, diethylaminoethyl; DUA, 4-deoxy-a- L -threo-hex-4-enopyranosyl uronic acid; GAG, glycosaminoglycan; GlcA, glucuronic acid; GlcN, glucosamine; HA, hydroxyapatite; IdoA, ... chromatography (Fig. 1). The salt-active acharan sulfate lyase activity fraction was then further purified to homogeneity by a series of hydroxyapatite Ultrogel ch...

Ngày tải lên: 23/03/2014, 21:20

... transfer of the acetyl group from N-acetylornithine into glutamate yielding ornithine and N-acetylglutamate. Glutamate N-acetyltransferase therefore recycles the acetyl moiety of N-acetyl- ornithine, ... acetyl- recycling pathway, both the first- and the second-step enzymes, N-acetylglutamate synthase and N-acetylglut- amate kinase, respectively, are inhibited by arginine. Glutamat...

Ngày tải lên: 20/02/2014, 03:20

... lectin agglutinated only a limited range of erythrocytes. Out of 12 erythrocyte types tested the lectin could agglutinate only six erythrocyte types (Table 2). Our study on the sialoconjugates ... evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of purificat...

Ngày tải lên: 21/02/2014, 00:20