Báo cáo Y học: Puriﬁcation and characterization of two secre

Báo cáo Y học: Puriﬁcation and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt

... Puriﬁcation and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato ( Lycopersicon esculentum ) cell cultures Gale G. Bozzo 1 , Kashchandra G. ... growth and secreted AP activity of tomato suspension cells Tomato cells cultured for 8 days in the absence of exogen- ous P i had only approximately 40% of the...

Ngày tải lên : 23/03/2014, 21:20

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Báo cáo Y học: Puriﬁcation and characterization of novel kininogens from spotted wolfﬁsh and Atlantic cod pdf

... Kalkkinen 1 1 Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland, 2 Norwegian College of Fishery Science, University of Tromsø, Norway, 3 Institute of Medical Biology, University ... biantennary and triantennary N-glycans that are terminated by a2,3-linked sialic acids, a high number of which are O-acetylated. About 1/3 of the glycans car...

Ngày tải lên : 08/03/2014, 23:20

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Báo cáo Y học: Puriﬁcation and characterization of three galactose speciﬁc lectins from Mulberry seeds (Morus sp.) ppt

... in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides and glycoproteins [1,2]. Plant lectins isolated from a wide variety of plants ... hemagglutinating activity, respectively. Although the yield of these proteins was found to be decreased by the puriﬁcation steps and over 96% of protein...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Puriﬁcation and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx

... mutagenesis [20], and later conﬁrmed by analysis of the crystal structure [19]. Comparison of the active site of VanXY C with VanX- and VanY-type enzymes indicated that all of these enzymes contained ... mass of 44 kDa. This method yielded 3–4 mg of His 6 –VanXY C from a culture volume of 1 L for enzyme characterization studies and was also used for puriﬁcation of...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc

... determination of hA2aR by X-ray or electron crystallography, or infor- mation on the bound ligand conformation obtained by NMR spectroscopy, would assist in the design of subtype speci®c compounds and ... Biosystems). Protein assay and amino -acid analysis Protein concentrations were determined by the Amido black assay [24] using BSA as a standard. For the puri®ed receptor, amino -ac...

Ngày tải lên : 24/03/2014, 00:21

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Báo cáo Y học: Puriﬁcation and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identiﬁcation as a product of liver origin doc

... (1990) Regulation and cellular localization of the membrane-bound thyrotropin-releasing h ormone-degrading enzyme in primary cultures of neur onal, glial and aden ohypophyseal cells. Endocrinology 127 , ... TRH-DE from rat and pig brain [28]. For the puriﬁcation of the serum TRH- DE fractionation by poly(ethylene glycol) precipitation was used as the ﬁrst step not only to pa...

Ngày tải lên : 31/03/2014, 21:21

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Báo cáo Y học: Puriﬁcation and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx

... to homogeneity with yield of 2.37% by a series of CM-Sepha- dex C-50 column chromatography (Fig. 1), hydroxyapatite column and Sephacryl S-300 gel filtration chromatography, and its final specific activity ... each enzyme with trypsin (Table 3). The internal sequences of chondroitin ABC and AC lyase show significantly greater homology of 59 and 80% to Flavobacterial chondroitin AC...

Ngày tải lên : 31/03/2014, 23:20

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Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... dhkim@khu.ac.kr Abbreviations: CM, carboxymethyl; DEAE, diethylaminoethyl; DUA, 4-deoxy-a- L -threo-hex-4-enopyranosyl uronic acid; GAG, glycosaminoglycan; GlcA, glucuronic acid; GlcN, glucosamine; HA, hydroxyapatite; IdoA, ... chromatography (Fig. 1). The salt-active acharan sulfate lyase activity fraction was then further puriﬁed to homogeneity by a series of hydroxyapatite Ultrogel ch...

Ngày tải lên : 23/03/2014, 21:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... transfer of the acetyl group from N-acetylornithine into glutamate yielding ornithine and N-acetylglutamate. Glutamate N-acetyltransferase therefore recycles the acetyl moiety of N-acetylornithine, ... acetyl- recycling pathway, both the ﬁrst- and the second-step enzymes, N-acetylglutamate synthase and N-acetylglutamate kinase, respectively, are inhibited by arginine. Glutamat...

Ngày tải lên : 20/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... lectin agglutinated only a limited range of erythrocytes. Out of 12 erythrocyte types tested the lectin could agglutinate only six erythrocyte types (Table 2). Our study on the sialoconjugates ... evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of purificat...

Ngày tải lên : 21/02/2014, 00:20

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