Báo cáo Y học: Regulation of mammalian translation factors by nutrients pot

... Control of translation factors by nutrients (Eur. J. Biochem. 269) 5349 MINIREVIEW Regulation of mammalian translation factors by nutrients Christopher G. Proud Division of Molecular Physiology, School ... Thr70, the sites most profoundly affected by rapamycin, may be regulated by nutrients through the mTOR pathway. REGULATION OF 4E-BP1 BY AMINO ACIDS In...

Ngày tải lên: 23/03/2014, 21:20

... ADP-ribosylated by diphtheria toxin [25]. ADP-ribosylation inhibits the activity of eEF2. Phosphorylation of eEF2 inhibits its activity, in translo- cation and in poly(U)-directed polyphenylalanine synthesis [26,27], ... activity of eEF2 kinase. Regulation of eEF2 by cellular energy status As pointed out above, translation elongation is expensive in terms of metabolic energy...

Ngày tải lên: 23/03/2014, 21:20

... Science/Veterinary Medical Sciences, The University of Tokyo, Japan The Sp family is a family of transcription factors that bind to cis-elements in the promoter regions of various genes. Regulation of transcription ... identification by Bigey et al.ofmany Correspondence to K. K. Yokoyama, Gene Engineering Division, Department of Biological Systems, BioResource Center, Tsukuba I...

Ngày tải lên: 22/02/2014, 07:20

... Double-positive clones were purified by secondary and tertiary r ounds of screening and genomic DNA inserts were purified using poly(ethylene glycol) precipitation [26]. The phage inserts were mapped by restriction ... s described by Koike et al. [21] are controlled by still another regulatory sequence, as they initiate several hundreds of bp upstream of the start of transcript...

Ngày tải lên: 08/03/2014, 16:20

... ere lysed in Laemmli sa mple buffer a nd were resolved by 12% SDS/ PAGE. Phosphorylated pleckstrin w as detected by autoradiography. (B) Platelets were lysed by the addition of denaturating lysis ... study down -regulation of the RAS–ERK signalling p athway and other functions of RAS. Down -regulation of the RAS– ERK pathway may be a critical step in the process of end- stage...

Ngày tải lên: 08/03/2014, 16:20

... phosphatases in the regulation of yeast SAPK pathways, then fo cus on the properties of the protein phosphatases implicated in the mammalian SAPK systems. REGULATION OF SAPK SIGNAL PATHWAYS BY PROTEIN PHOSPHATASES ... phosphorylation of conserved tyrosine and threonine residues within the catalytic domain. This phosphorylation is mediated by dual specificity protein kinases,...

Ngày tải lên: 17/03/2014, 23:20

... glycosylphophatidylinositol (GPI) anchors [1,2]. Although the primary structure of the glypi- cans is only marginally conserved (about 30% identity), there is a strict conservation of 14 cysteine ... modulated by growth factors and cytokine [13,14]. In most cells, levels of syndecan synthesis correlate well with syndecan mRNA levels, suggesting that the regulation is mainly transc...

Ngày tải lên: 17/03/2014, 23:20

... acetic ether were used at the ratio of 2:1. The purity was estimated by use of thin-layer chromatography. Cleavage of the tert-butyl ester. 2 mmol tert-butyl pro- tected PNA building block functionalized ... 1. Synthesis of the PNA building block backbone In the next step, the synthesis of the PNA back- bone monomer using the fluorenylmethoxycarbonyl (Fmoc) protection occurre...

Ngày tải lên: 26/10/2012, 08:57

... Faculty of Pharmacy, University of Ljubljana, Slovenia 2 Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia 3 Department of Biology, Biotechnical Faculty, University of Ljubljana, ... obtained by native gel electrophoresis. Size exclusion chromatography The dissociation of cystatin C from the cathepsin B ⁄ cystatin C complex by 2A2 mAb was tested by siz...

Ngày tải lên: 18/02/2014, 11:20

... the major role played by nuclear factor-jB in the anti-inflammatory action of glutamine, the stimulatory role of activating protein-1 and the inhibitory role of C/EBP homology binding protein ... the physio- logical importance of glutamine which, by counter- acting activation of the NF-jB pathway, contributes to the attenuation of local inflammation in the gut and lung. The path...

Ngày tải lên: 07/03/2014, 00:20