Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot

... expression of the L. collinoides diol dehydratase The three genes pduCDE encoding the three subunits of the diol dehydratase were cloned in the pQE30 expression vector system. The synthesis of the three ... study, we report the purification, enzymatic characterization and analysis of the composition of the diol dehydratase of L. collinoide...

Ngày tải lên: 23/03/2014, 21:20

... hydrogen bonds with the thymine or adenine bases. The central N5(D) atom of the drug is hydrogen-bonded to the two O2 atoms of the thymine bases of the central AT steps. Nitrogen atoms N3(D) and ... drug hydrogen-bonds with the bases of only one DNA strand and stacks with the sugar rings. We have previously reported the structure determin- ation at 1.9-A ˚ resolu...

Ngày tải lên: 24/03/2014, 04:21

... ATPase activity at either pH 7.4 or pH 6.4 (Fig. 3). At pH 7.4, 5m M azide inhibited ADP hydrolysis by  70% whereas ATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide ... apical membranes of the oxyntic-peptic cells [37]. The distribution of the ecto- ATPDase on these epithelial cells is distinctly different from theotherATPDaseintheE-ATPase...

Ngày tải lên: 22/02/2014, 04:20

... method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, maltose and cyclohexaamylose b-amylase activity was measured using the iodine ... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, the active site of the C. sepium b-amylase most probabl...

Ngày tải lên: 22/02/2014, 07:20

... (PLP)-dependent enzyme which catalyses the b,c- hydrolytic cleavage of the amino acids L -kynurenine (1 )and L -3-hydroxykynurenine (2)togive L -alanine (3) and either anthranilic acid (4) or 3-hydroxyanthranilic ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan– kynurenine metabolic pathway. The purifica...

Ngày tải lên: 08/03/2014, 22:20

... (saponification) of the N-glycans yielded glycans devoid of additional acetyl groups, an indication that these were O-acetyl substituents [15]. The MALDI-TOF spectrum of the de-O-acetylated glycans reveals the ... belonging to class 3 of the cystatin superfamily. They are single-chain proteins composed of an N-terminal heavy chain, the bradykinin moiety and a C-terminal...

Ngày tải lên: 08/03/2014, 23:20

... galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the protein was detected by periodic ... In the other side of the tank, a light source was placed in order to attract the nauplii. Two days were allowed for the hatching of all the eggs an...

Ngày tải lên: 18/03/2014, 01:20

... VanX- and VanY-type enzymes indicated that all of these enzymes contained the same Zn 2+ binding motif. VanX homologues present in the cyanobac- terium Synechocystis strain PCC6803, and the glycopeptide antibiotic ... a D -Ala- D -Ser standard curve. For determination of the rate of hydrolysis of UDP-MurNAc- pentapeptide[Ala], His 6 –VanXY C was used at a concentra- tion...

Ngày tải lên: 18/03/2014, 01:20

... below. AP assays All assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... °C. Phosphatase assay A For routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactate dehydrogenase reactio...

Ngày tải lên: 23/03/2014, 21:20

... determination of hA2aR by X-ray or electron crystallography, or infor- mation on the bound ligand conformation obtained by NMR spectroscopy, would assist in the design of subtype speci®c compounds and ... cally to the XAC-agarose. Theophylline was removed by gel ®ltration before quanti- fying speci®c [ 3 H]ZM241385 binding in the X AC-agarose eluate. The value obtained was onl...

Ngày tải lên: 24/03/2014, 00:21