Báo cáo Y học: Modeling the three-dimensional structure of H+-ATPase of Neurospora crassa Proposal for a proton pathway from the analysis of internal cavities pptx

... 2002 Modeling the three-dimensional structure of H + -ATPase of Neurospora crassa Proposal for a proton pathway from the analysis of internal cavities Olivier Radresa 1 , Koji Ogata 2 , Shoshana Wodak 2 , ... putative proton pathway in Neurospora crassa H + -ATPase The chemiosmotic model for PMA1_NEUCR. In the P-type proton pumps, the or...

Ngày tải lên: 23/03/2014, 21:20

Ngày tải lên: 02/11/2012, 10:09

... 5¢- TCCATAGCAGCGGCGAAACCA-3¢ and chromosomal DNA from strain LM1237 [17] as a template. The PCR fragment was subsequently digested with the enzymes DraI and EcoRV and a 842-bp fragment containing the ... is temperature sensitive, and that the pA1lacO-1 promoter and the lacI q1 gene are surrounded by a DNA fragment originating from upstream the gyrA gene and a fragment...

Ngày tải lên: 18/03/2014, 01:20

... released from transfected COS7 cells by adding heparin to the media, was assayed for catalytic activity. The lipase (A) and esterase (B) activities of the substituted mutants, R40 5A, K41 3A, K41 3A/ K41 4A, ... nm was determined at an excitation wavelength of 386 nm. Oleic acid was used as a standard for free fatty acid. Esterase activity was measured using p-nitrophenyl b...

Ngày tải lên: 21/02/2014, 15:20

... that not only verbal predicates are semantically important, they also form a major part of the sentences. Therefore, whether verbal predicates are trans- lated correctly or not has a great impact ... verbal predicate, the translation of the head word of the argument, as well as the boundary words of the translation of the argument are used as features. 4.3 Training...

Ngày tải lên: 19/02/2014, 19:20

... and A5 3T mutants of a- synuclein that cause f amilial c ases of Parkinson's disease. Unfortunately the quality of s ome of these syn uclein ROA spectra i s generally not as good as that of ... were also attempted on a S1 -and a S2 -casein, these proteins had a tendency to aggregate in the laser beam, which prevented the acquisition of ROA data of suf®cient qu...

Ngày tải lên: 21/02/2014, 15:20

... cid (GlcA)andgalacturonicacid(GalA)intheratio 1:5. Analysis on an amino-acid analyser showed the presence o f 2-amino-2-deoxygalactose and lysine. The D configuration of GalA and GalN was determined by GLC of ... the acetylated (S)-2-butyl glycosides and the L configuration of lysine by GLC of the acetylated (S)-2-butyl ester. The D configuration of GlcA was established...

Ngày tải lên: 21/02/2014, 15:20

... set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration are ... in the starting structure. Although mathemat- ical convergence may be achieved by iterative calculations, the accuracy of the distances obtained remains uncertain. Th...

Ngày tải lên: 22/02/2014, 07:20

... C-terminal domains, that produce the dramatically increased affinity for R*; on the other hand, binding of the phosphopeptide may simply alter the surface features and cause relatively minor conformational ... implemented in the program KALEIDAGRAPH (v3.08, Synergy Software), setting the molecular mass of the monomer equal to 45.3 kDa and the forward scattering of the...

Ngày tải lên: 22/02/2014, 07:20

... was adopted for the elution of the peptide. The fraction containing the pure peptide was lyophilized twice and the purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF ... be described as a random coil, with only a small population of local nonrandom structures. Overall, these data indicate that bulk water is not suitable for high resolut...

Ngày tải lên: 08/03/2014, 09:20