Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp RAG-1 docx

... Biology, University of Maryland, USA An extracellular lipase, LipA, extracted from Acinetobacter sp. RAG-1 grown on hexadecane was purified and proper- ties of the enzyme investigated. The enzyme ... biocatalyst. Keywords: lipase; LipA; Acinetobacter sp. RAG-1; protein purification; zymogram. Lipases are glycerol ester hydrolases (EC 3.1.1.3) that catalyze the hydrol...

Ngày tải lên: 23/03/2014, 21:20

... amino-acid sequence. The hydrolytic speci®city of the enzyme When the hydrolytic activities of the proteinase were measured at pH 9.0 with various synthetic substrates, the results listed in ... of the s tability features of the enzyme may be affected by small amounts of contaminating proteins. Fig. 3. The eect of pH on the proteinase activity, measured with th...

Ngày tải lên: 31/03/2014, 21:21

... (PLP)-dependent enzyme which catalyses the b,c- hydrolytic cleavage of the amino acids L -kynurenine (1 )and L -3-hydroxykynurenine (2)togive L -alanine (3) and either anthranilic acid (4) or 3-hydroxyanthranilic ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan– kynurenine metabolic pathway. The purific...

Ngày tải lên: 08/03/2014, 22:20

... encodes the catalytic subunit (CooS) of the CO dehydrogenase, an electron transfer protein (CooF) of the CO dehydrogenase, and proteins required for the insertion of Ni into the enzyme (CooC, T, and ... tightly associated with the hydrogenase. Catalytic properties of the enzyme complex The purified CO-oxidizing:H 2 -evolving enzyme complex is composed of a hydro...

Ngày tải lên: 23/03/2014, 21:20

... from the tissues of carnation (Dianthus caryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxy substituent in 4¢-position of the flavones, ... regarding the role of this enzymatic activity in the biosynthesis of methylated phenols other than the dianthramide-derivatives. In this respect, the object of...

Ngày tải lên: 08/03/2014, 08:20

... (saponification) of the N-glycans yielded glycans devoid of additional acetyl groups, an indication that these were O-acetyl substituents [15]. The MALDI-TOF spectrum of the de-O-acetylated glycans reveals the ... belonging to class 3 of the cystatin superfamily. They are single-chain proteins composed of an N-terminal heavy chain, the bradykinin moiety and a C-terminal...

Ngày tải lên: 08/03/2014, 23:20

... galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the protein was detected by periodic ... In the other side of the tank, a light source was placed in order to attract the nauplii. Two days were allowed for the hatching of all the eggs an...

Ngày tải lên: 18/03/2014, 01:20

... VanX- and VanY-type enzymes indicated that all of these enzymes contained the same Zn 2+ binding motif. VanX homologues present in the cyanobac- terium Synechocystis strain PCC6803, and the glycopeptide antibiotic ... specific- ally hydrolyse D -Ala- D -Ala with minimal activity against D -Ala- D -Ser, a very different type of specificity. VanXY C , VanX-type and VanY-type enzym...

Ngày tải lên: 18/03/2014, 01:20

... activation of the enzyme (125 and 118% yield as compared with the activity after the Matrix Red column and 108 and 113% as compared with the crude extract for control and anoxic enzymes, respectively). ... summarizes the purification of turtle liver GSTs using Matrix Red dye ligand chromatography, Sephacryl S-200 gel filtration and hydroxylapatite ion exchange chromato...

Ngày tải lên: 23/03/2014, 15:20

... below. AP assays All assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... °C. Phosphatase assay A For routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactate dehydrogenase reactio...

Ngày tải lên: 23/03/2014, 21:20