Báo cáo khoa học: The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH docx

... The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH Emeline Lagarrigue1, Diane Ternent2, Sutherland K. Maciver2, Abdellatif Fattoum3, Yves ... induced by lowering pH does not allow atight interaction of G4–6 with G-actin.Discussion The pH- dependence of the G2/G4–6 interface Activation of gelsolin by calcium and/or low pH has beenfound to ... induced by calcium, important differences exist. The most strikingdifference is that the calcium latch between G2 and 6 that is released by calcium is not released by lowered pH. Also, the actin-binding...

Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

... catalyses the phosphorylation of a-d-glucose (Glc) to form glucose 6-phosphate, usingMgATP2)as the phosphoryl donor. It is a key regula-tory enzyme in the pancreatic b-cell [isoform 1 of human ... states of hGK [12], the residue contacts that make essential con-tributions to the binding of Glc to the super-open con-formation have not been identiﬁed. The characterization of these residues is ... maychange the equilibrium between the two proposed GKconformers (Eqn. 1B), but the accuracy of the experi-ments was hindered by the low signal amplitude [24].Therefore, they also studied the equilibrium...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... conservedmotif of Gly–Gly–X–Gly. The second glycine of thismotif (Gly374) is known to make a hydrogen bondwith the pyrophosphate moiety of the FAD of the nextmonomer in the tetramer [16]. However, the ... t-test)(data not shown). Therefore, it is likely that the differ-ence in the regulation of BmIVD expression between+skuand sku alleles is not responsible for the odorousphenotype. To further characterize ... combination of or and sku phenotypes. The lowerpart indicates the representative results of genomic DNA directsequencing of PCR products harbouring the SNP 1127G>T of BmIVD from each of the three phenotypes.Table...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... popula-tion. The inﬂammatory process is characterized by inﬁltration of inﬂammatory cells into the joints, leading to the proliferation of ﬁbroblast-like synoviocytes (FLS)and the destruction of cartilage ... polyarticular joint dis-ease that primarily affects the small joints of the hands and feet. The inﬂammatory process is characterized by inﬁltration of inﬂammatory cellsinto the joints, leading to proliferation ... Onecharacteristic feature of chemokines is the redundancy of the system. Several chemokines bind to more thanone receptor, and the majority of chemokine receptorshave multiple ligands, leading to the...

Tài liệu Báo cáo khoa học: Inﬂuence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx

... modulate the fundamental biophysicalprotein properties. By applying this methodology to the study of the kinetics of catalysis by the serine pro-tease a-CT we were able to statistically correlate the structurally ... directions. From this analysis the dynamics of the protein are described by a set of N)1normal modes, the ktheigenvector (uk)ofG gives the residue displacements alongmode k, and the ktheigenvalue, ... highlight the large contribution that the internal electrostatics have towards decreasing the total energy of the conjugates, which agrees with the hypothesis of global electrostatics being relevant to Table...

Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

... (EGF),which bind to dedicated receptors. Upon EGF binding, itsreceptor (EGFR) dimerizes, leading to autophosphoryla-tion of tyrosine residues on the cytoplasmic domain of the receptor, thereby creating ... timepoint of 60 min in order to measure accurately the synergistic effect.EGF concentration-dependency of synergistic activation To investigate the synergistic activation i n the second phase of the ... circuit is not involved in the s ynergistic ERK phosphorylationcaused by EGF and PMA.Discussion The architecture of signal transduction networks i s oftenhighly complex, due to the large number of...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... 2002 The proton signal of His57 Ne2-Ser194 Oc was alsodetected at around 14.0 p.p.m. at pH 9.1–6.9. The His57 Ne2 proton signal disappeared below pH 6.0 due to the protonation of His57 Ne2. These ... the pKa of His210 is 2 pH units higher than that of His57 is the buried charge interaction with Asp113. The shieldingeffect of Asp113-His210 by Trp169 was supported by the independency of the ... may be too fast to monitor as the split signals,while at low temperature, that of the interchange rate is tooslow to monitor as the single one.Fig. 2. The relative pH- activity proﬁles of wild-type...

Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt

... [11]. In another, noncompetitiveinhibitors with IC50values of 0.1 lm were found, but these were prone to hydrolysis with deactivation half-lives of 1–2 h. The latter are thought to bind to NS3pro, ... identiﬁed low molecularmass inhibitor. Our results show that all these inhibitors act by occupying the substrate-binding site of the protease rather than by an allosteric mech-anism. In addition, the ... inhibitors and BPTI, the proteolytically most active conformations arethought to be represented by the structures observedwith inhibitors rather than the one without inhibitor. The function of the...

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and triﬂuoromethionine, a promising lead compound against amoebiasis ppt

... proteins in the cytosol of the parasite,leading to the observed toxicity to the cell. The fact that EhMGL2, which is more active in the degradation of TFM, is less sensitive than EhMGL1seems to contradict ... isozymes, and that the degradation of TFM is less sensitive to alterations of these residues than is the degrada-tion of physiological substrates. These results support the use of TFM to target MGL.AbbreviationsCS, ... the notion that the product of the degradation is the enzyme inactivator. However, wespeculate that the distribution of possible primaryamines, which are target of the TFM adducts (carbo-nothionic...

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... v) Triton X-100, pH 8 [18]. At 30 °C, the oxidation of the substrate DHO with the quinone cosub-strate was coupled to the reduction of the chromogen 2,6-di-chloroindophenol. The Km of DHO ... proteins of family 2 must be translo-cated from the cytosol to the inner membrane of mito-chondria. The proteins are directed by targetingsequences, which usually consist of various numbers of amino ... respiratory chain complexes, the assemblyensures the transfer of electrons from different sources to oxygen by the respiratory chain complexes and the coupling of proton uptake from the matrix...

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