Báo cáo khoa học: The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH docx

... The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH Emeline Lagarrigue 1 , Diane Ternent 2 , Sutherland K. Maciver 2 , Abdellatif Fattoum 3 , Yves ... induced by lowering pH does not allow a tight interaction of G4–6 with G-actin. Discussion The pH- dependence of the G2/G4–6 interface Activation of gel...

Ngày tải lên: 23/03/2014, 21:20

... catalyses the phosphorylation of a-d- glucose (Glc) to form glucose 6-phosphate, using MgATP 2) as the phosphoryl donor. It is a key regula- tory enzyme in the pancreatic b-cell [isoform 1 of human ... states of hGK [12], the residue contacts that make essential con- tributions to the binding of Glc to the super-open con- formation have not been identified. The...

Ngày tải lên: 30/03/2014, 04:20

... conserved motif of Gly–Gly–X–Gly. The second glycine of this motif (Gly374) is known to make a hydrogen bond with the pyrophosphate moiety of the FAD of the next monomer in the tetramer [16]. However, the ... t-test) (data not shown). Therefore, it is likely that the differ- ence in the regulation of BmIVD expression between + sku and sku alleles is not r...

Ngày tải lên: 18/02/2014, 04:20

... popula- tion. The inflammatory process is characterized by infiltration of inflammatory cells into the joints, leading to the proliferation of fibroblast-like synoviocytes (FLS) and the destruction of cartilage ... polyarticular joint dis- ease that primarily affects the small joints of the hands and feet. The inflammatory process is characterized by infiltration of...

Ngày tải lên: 18/02/2014, 18:20

... modulate the fundamental biophysical protein properties. By applying this methodology to the study of the kinetics of catalysis by the serine pro- tease a-CT we were able to statistically correlate the structurally ... directions. From this analysis the dynamics of the protein are described by a set of N )1 normal modes, the k th eigenvector (u k )ofG gives...

Ngày tải lên: 19/02/2014, 05:20

... (EGF), which bind to dedicated receptors. Upon EGF binding, its receptor (EGFR) dimerizes, leading to autophosphoryla- tion of tyrosine residues on the cytoplasmic domain of the receptor, thereby creating ... time point of 60 min in order to measure accurately the synergistic effect. EGF concentration-dependency of synergistic activation To investigate the synergisti...

Ngày tải lên: 19/02/2014, 16:20

... 2002 The proton signal of His57 Ne2-Ser194 Oc was also detected at around 14.0 p.p.m. at pH 9.1–6.9. The His57 Ne2 proton signal disappeared below pH 6.0 due to the protonation of His57 Ne2. These ... the pK a of His210 is 2 pH units higher than that of His57 is the buried charge interaction with Asp113. The shielding effect of Asp113-His210 by Trp169 was s...

Ngày tải lên: 21/02/2014, 03:20

... [11]. In another, noncompetitive inhibitors with IC 50 values of 0.1 lm were found, but these were prone to hydrolysis with deactivation half- lives of 1–2 h. The latter are thought to bind to NS3pro, ... identified low molecular mass inhibitor. Our results show that all these inhibitors act by occupying the substrate-binding site of the protease rather than by an al...

Ngày tải lên: 07/03/2014, 02:20

... proteins in the cytosol of the parasite, leading to the observed toxicity to the cell. The fact that EhMGL2, which is more active in the degradation of TFM, is less sensitive than EhMGL1 seems to contradict ... isozymes, and that the degradation of TFM is less sensitive to alterations of these residues than is the degrada- tion of physiological su...

Ngày tải lên: 07/03/2014, 05:20

... v) Triton X-100, pH 8 [18]. At 30 °C, the oxidation of the substrate DHO with the quinone cosub- strate was coupled to the reduction of the chromogen 2,6-di- chloroindophenol. The K m of DHO ... proteins of family 2 must be translo- cated from the cytosol to the inner membrane of mito- chondria. The proteins are directed by targeting sequences, which usuall...

Ngày tải lên: 07/03/2014, 12:20