Báo cáo khoa học: Binding affinity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity pot
Báo cáo khoa học: Binding affinity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity pot
... Biochem. 270) Ó FEBS 2003 Binding affinity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity Chieka Minakuchi 1 , ... ecdysone agonists to the ecdysone receptor ultraspiracle complex primarily determines the strength of their molting hormonal activity...
Ngày tải lên: 23/03/2014, 21:20
Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx
... components of the recep- tor’s high -affinity Man-6-P binding sites. The structure of repeats 1–3 of the bovine M6P ⁄ IGF2R in the pres- ence of Man-6-P was solved by Olson et al. [18]. Their Ligand binding ... tags, of which only one is tethered to the resin bead) demonstrate complex binding behavior. Dimers of this type exhibit the predicted amount of bi...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... occured. A binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macromolecule ratio. From the binding isotherm values for the reaction stoichiometry, ... picomolar affinity for copper but at pH 5 the affinity is in the nanomolar range. There is no difference in the affinity of copper between the...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans potx
... transferred by GnT-IV abolished the binding affinity (105, 204). However, addition of the bisecting GlcNAc (104) abolished the binding in the case of LSECtin. The specificity of the DC-SIGN- related lectins ... In the absence of 2 mm CaCl 2 , adhesion of these transfected CHO cells to Lec8 cells was reduced to the level of control CHO cells (Fig. 6C). These res...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.) ppt
... observations highlight the inadequacies in our current understanding of the relevance of HSEs in HS response in rice. The formation of the trimeric form of Hsfs is con- sidered important for attaining their high affinity ... visualization of the subcel- lular locations of the interacting proteins in the cell [6,32]. Subsequently, OsHsfA2c and OsHsfB4b were analyze...
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa học: Mitochondrial affinity for ADP is twofold lower in creatine kinase knock-out muscles Possible role in rescuing cellular energy homeostasis pptx
... Import- antly, the data show that there is no significant change in Mi-CK activity in the case of M-CK deficiency. The total CK activity was significantly lower in the heart homogenate of the three CK-deficient ... rotenone. The RCR value (A) is the ratio of state 3 over state 4 (data not shown). The ACR value (B) is the ratio of state 3 over the atractyloside-inhi...
Ngày tải lên: 30/03/2014, 15:20
Báo cáo Y học: High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences doc
... be mentioned that the ionization state of amino-acid residues is influenced by their unique microenvironment; therefore, predicting the impact of the residues based solely on theoretical pK a of their individual ... wells. To further localize the domain of laminin responsible for LBP binding, we took advantage of the fact that a number of peptides responsible for th...
Ngày tải lên: 08/03/2014, 23:20
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... [18]. Taken together, the results of various signal intensi- ties of the three PrP C bands are accredited to the development of the truncated isoforms, to the epitope recognition of the antibodies ... densitometry. The mean values of the calculated signal intensities were analyzed for each of the N- or C-terminal binding antibodies. The banding pattern of...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... bound to the rubredoxin and the effect observed in the signals of the haem methyls of DvHc 3 :ZnRb complex vs. the results in the isolated cytochrome is close to the full effect of complex forma- tion. ... Experimental measure- ments of the reduction potentials of proteins involved in complexes have been reported [5–7], but the effect of partner binding on...
Ngày tải lên: 19/02/2014, 17:20
Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... insight into the dynamics of the binary complex with ATP, including motion of the flexible surface ⁄ active site loop and partial closure of the active site cleft. In the complex, the adenosine ... consistent with the observed disorder in the crystal structure. MD simulations of the mod- elled binary GK–ATP complex revealed that the global rmsd of the struc...
Ngày tải lên: 06/03/2014, 00:20