Báo cáo khoa học: Binding afﬁnity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity pot

... Biochem. 270) Ó FEBS 2003 Binding afﬁnity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity Chieka Minakuchi1, ... ecdysone agonists to the ecdysone receptor ultraspiracle complex primarily determines the strength of their molting hormonal activity. Keywords: ecdysone receptor; dibenzoylhydrazines; nuclear receptor; receptor ... the ecdysone receptor ultraspiracle complex for a series of compoundswas highly correlated with their molting hormonal activity, indicating that the binding aﬃnity of nonsteroidal ecdysone agonists...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... components of the recep-tor’s high -afﬁnity Man-6-P binding sites. The structure of repeats 1–3 of the bovine M6P ⁄ IGF2R in the pres-ence of Man-6-P was solved by Olson et al. [18]. Their Ligand binding ... tags, of which only one is tetheredto the resin bead) demonstrate complex binding behavior. Dimers of this type exhibit the predictedamount of binding only if the heterodimer is tetheredby the ... employing solubleforms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma-tion in the absence of ligand. Afﬁnity cross-linking of [125I]IGF-II to...

Tài liệu Báo cáo khoa học: High afﬁnity copper binding by steﬁn B (cystatin B) and its role in the inhibition of amyloid ﬁbrillation docx

... occured. A binding isothermwas ﬁtted to the data, expressed in terms of the heat changeper mole of ligand against the ligand to macromoleculeratio. From the binding isotherm values for the reactionstoichiometry, ... picomolar afﬁnity for copperbut at pH 5 the afﬁnity is in the nanomolar range. There is no differencein the afﬁnity of copper between the wildtype steﬁn B (E31 isoform) and avariant (Y31 isoform), ... by subtraction of data from a series of injections of copper chelate solutioninto a buffer blank correlating to the heat of dilution of the copper complex. After subtraction of the blank data...

Báo cáo khoa học: Frontal afﬁnity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for afﬁnity to agalactosylated N-glycans potx

... transferred by GnT-IV abolished the binding afﬁnity (105, 204). However, addition of the bisecting GlcNAc (104) abolished the binding in the case of LSECtin. The speciﬁcity of the DC-SIGN-related lectins ... In the absence of 2 mm CaCl2,adhesion of these transfected CHO cells to Lec8 cellswas reduced to the level of control CHO cells (Fig. 6C).These results, together with the results of the ... aAGP in the absence of competitors.In the presence of either of these monosaccharideinhibitors, binding of DC-SIGN-related lectin–Fcchimeras to agalactosylated aAGP was inhibited.These results...

Báo cáo khoa học: Binding afﬁnities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.) ppt

... observationshighlight the inadequacies in our current understanding of the relevance of HSEs in HS response in rice. The formation of the trimeric form of Hsfs is con-sidered important for attaining their high afﬁnity ... visualization of the subcel-lular locations of the interacting proteins in the cell[6,32]. Subsequently, OsHsfA2c and OsHsfB4b wereanalyzed for their potential to form homomers by the Temp (oC)EGSHsfA922 ... afﬁnity with the select group of OsHsfstested herein. Because lower afﬁnity sites contribute in the binding of transcription factors and gene regula-tion [37], the low afﬁnity binding noted...

Báo cáo khoa học: Mitochondrial afﬁnity for ADP is twofold lower in creatine kinase knock-out muscles Possible role in rescuing cellular energy homeostasis pptx

... Import-antly, the data show that there is no signiﬁcant changein Mi-CK activity in the case of M-CK deﬁciency. The total CK activity was signiﬁcantly lower in the heart homogenate of the three CK-deﬁcient ... rotenone. The RCR value (A) is the ratio of state3 over state 4 (data not shown). The ACR value (B) is the ratio of state 3 over the atractyloside-inhibited state. For the determination of apparent ... implications of the identi-ﬁed Vmaxand KADP50adaptations of mitochondriawith respect to the function of the integrated ATPasenetwork of the active striated muscle cell in which spe-ciﬁc CK isoforms...

Báo cáo Y học: High afﬁnity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-ﬁnger like sequences doc

... bementioned that the ionization state of amino-acid residues isinﬂuenced by their unique microenvironment; therefore,predicting the impact of the residues based solely ontheoretical pKa of their individual ... wells. Tofurther localize the domain of laminin responsible for LBP binding, we took advantage of the fact that a number of peptides responsible for the attachment activity for a variety of cell ... Preincubation of LBP with either Zn2+or EDTA (Fig. 3C) did not alter the binding activity suggesting thereby that the cofactor requirement of Zn2+isfor laminin only. Treatment of l aminin...

Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

... [18].Taken together, the results of various signal intensi-ties of the three PrPCbands are accredited to the development of the truncated isoforms, to the epitoperecognition of the antibodies ... densitometry. The mean values of the calculatedsignal intensities were analyzed for each of the N- or C-terminal binding antibodies. The banding pattern of the di- (d), mono- (j)and nonglycosylated isoform ... corres-ponding to the size of the nonglycosylated full-lengthprotein indicated antibody binding at the structuredcore region of PrPCas the result of an overlap of twoproteins, the nonglycosylated...

Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx

... bound to the rubredoxin and the effectobserved in the signals of the haem methyls of DvHc3:ZnRb complex vs. the results in the isolatedcytochrome is close to the full effect of complex forma-tion. ... Experimental measure-ments of the reduction potentials of proteins involvedin complexes have been reported [5–7], but the effect of partner binding on the microscopic properties of the redox centres ... of the haem methyl groups in the fully reduced stage 0 are not plotted because they are unaffected by the pH. The solid lines represent the best ﬁt of the shifts for DvHc3:Pi to the model of...

Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc

... insight into the dynamics of the binary complex with ATP,including motion of the ﬂexible surface ⁄ active site loop and partial closure of the active site cleft. In the complex, the adenosine ... consistent with the observed disorderin the crystal structure. MD simulations of the mod-elled binary GK–ATP complex revealed that the globalrmsd of the structure converged at the end of the 2-nssimulation ... indicated by the MD simulations of the modelled ternary hGK–Glc–ATP complex. In the ﬁnal structure of the simulations, the interactions of the adenosine moiety were similarto those observed in the binary...

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