Báo cáo khoa học: Redox properties of cytochrome P450BM3 measured by direct methods potx

... Redox properties of cytochrome P450 BM3 measured by direct methods Barry D. Fleming 1 , Yanni Tian 1 , Stephen G. Bell 1 , Luet-Lok ... catalysed the reduction of oxygen to hydrogen peroxide. Keywords: cytochrome P450 BM3 ; redox properties; electro- chemistry. The cytochrome P450 group of enzymes comprises a variety of heme-containing ... line) later. The arrows...

Ngày tải lên: 23/03/2014, 21:20

... hydro- phobic tails, followed by a translation, of $ 2A ˚ ,of each layer in a direction perpendicular to the disc’s plane, thereby providing an approximate restoration of the bilayer interface, without ... Alignment of the long loop region in the calf- 2 module of integrin’s a subunits. Fig. S2. A gallery of structures predicted by Robetta [20] for the long loop in the calf-2...

Ngày tải lên: 18/02/2014, 04:20

... effects of oxidation and acts as a redox sensor [8,13,32]. Oxidation of RPTPa-D2, like PTP1B, results in the formation of cyclic sulfenamide at the catalytic site, which is stable and reversible by ... role of oxidation in dimerization of a panel of four different RPTPs. We compared four RPTPs from different subtypes, i.e. RPTPa, RPTPl, CD45 and LAR. We found that the cytoplas...

Ngày tải lên: 18/02/2014, 17:20

... of cytoplasmic composition of anionic li- pids in membrane binding of K-Ras was evaluated by studying the effect of ATP depletion, which inhibits inward movement of PS, with consequent loss of plasma ... the role of electrical properties of the plasma membrane in the subcellular distribution of K-Ras. In particular, we investigated (a) the role of surface charge on inn...

Ngày tải lên: 19/02/2014, 00:20

... Loss of inhibitory potential of dimerized cystatin F can be explained by blocking of the N-terminal part, disabling protease access, or by a conformational change result- ing from disruption of ... dilution, cystatin F was equilibrated with a twofold molar excess of papain. The residual activity of papain was measured with Z-FR-AMC. Rel- ative activity of cystatin F is sho...

Ngày tải lên: 19/02/2014, 17:20

... concentrations were calculated directly by means of a pNP calibration curve in the case of the colorimetric assay, or by using a (GlcNAc) 2 calibration curve in the case of quantita- tive HPLC-MS. ... binding site of chitinase A was probably composed of an array of six binding subsites. Point mutations were introduced into two active site residues – Glu315 and Asp392 – by si...

Ngày tải lên: 20/02/2014, 01:20

... of GST-UBC9 as indicated (given as ng of UBC9 only). The amount of sumoylated Myb species were quantified by the NIH IMAGE 1.62 software (upper panel) and the different sumoylated Myb species measured ... transactivation properties. Such changes could occur directly, by modula- tion of Myb’s intrinsic activation potential, or indirectly through changes in subnuclear associations....

Ngày tải lên: 21/02/2014, 00:20

... molecule in a wide range of organisms. The structure of cytochromes c¢ exhibits clear differ- ences from that of the well-known Ambler’s class I cytochromes c. The class I cytochromes c are spherical proteins ... the latter. Heterologous synthesis of PHCP by E. coli The E. coli System I cytochrome c biogenesis machin- ery, consisting of the Dsb and Ccm proteins, is respon- sible...

Ngày tải lên: 06/03/2014, 00:20

... be induced by at least three different methods: firstly, by proteolytic cleavage of the N-terminus, resembling the activation of phenoloxidase by the phenoloxidase cas- cade [16,17]; secondly, by complex ... characterization of the enzymatic properties is still lacking. In this article, the enzymatic properties of activated hemocyanin from the tarantula Eurypelma californi...

Ngày tải lên: 07/03/2014, 05:20

... was observed and identified by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated ... of the monomeric form of SV-IV is approximately doubled by the addition of 5.4 m M SDS (Fig. 1), whereas in the 1–70 region, the a-helix content increases from 2–3% to  23% in th...

Ngày tải lên: 07/03/2014, 14:20