Báo cáo khoa học: Effect of mutations at Glu160 and Val198 on the thermostability of lactate oxidase doc

... doi:10.1046/j.1432-1033.2003.03751.x lactate oxidases to investigate the effect of an E160G/V198I double mutation 4 on thermostability and enzyme activity. Materials and methods Construction of the mutant lactate oxidase gene The ... in the thermal-inactivation curves of the V198I mutant and wild-type lactate oxidases (Fig. 2). This indicates that the V19...

Ngày tải lên: 23/03/2014, 21:20

... stabilize the homodimers (data not shown). These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientation between the CD38 monomers. It ... extended expression on the plasma membrane. Enzyme activity is not dependent on the presence of stable homodimers As the mutations in the cytoplasmic regio...

Ngày tải lên: 23/03/2014, 12:20

... issues; Section 5 quantitatively analyses the different contextual feature types or feature types combination in the view of information theory and draws a series of conclusion on their predictive ... information redundancy can be used as a measure of the redundancy between the predictive information of a feature type and that of a feature type combination. Predictiv...

Ngày tải lên: 20/02/2014, 18:20

... Reduplication is the verbal reduplication morpheme. Of interest in this example are regressive Vowel Harmony 4, and, of course, reduplication. The input consists of the stream of segments and ... separate word n this is the case with cliticization. Also exemplary of the division of duty between the morphophonological parser and the syntactic parser is th...

Ngày tải lên: 08/03/2014, 18:20

... a result of the loss of energetic contributions of alanine- substituted residues rather than the effect of such mutations on protein structure. In summary, the fact that the combined loss of free energy ... elaborate on these key insights into the role of cooperativity in driving protein–DNA interac- tions, we also analyzed the energetic contributions of alan...

Ngày tải lên: 22/03/2014, 16:20

... trafficking of NCT. Effect of Synoviolin on the production of Ab NCT is one of the essential cofactors of the c-secretase complex. We therefore investigated the effect of the Synoviolin-mediated degradation ... most likely that the accumulation of NCT in synoviolin-null cells is a result of the suppression of the degradation of NCT. To further investig...

Ngày tải lên: 23/03/2014, 04:20

... [13,14]. This effect generates a heat-shock response in the cell [15,16]. Maximal production of CIII prolongs the heat-induced synthesis of E. coli heat shock proteins even at low temperature [17]. The ... proceed without ATP. In this respect, r 32 resembles kCII. Inhibition of HflB-mediated proteolysis of r 32 by CIII and by CIIIC The effect of CIII and CIIIC on...

Ngày tải lên: 23/03/2014, 10:20

... that the effect of on the interpolated probability becomes large when the confidence of the estimate of the conditional probability estimated from the feedback corpus is small (that is, when there ... types of : , , and that denote the contexts consisting of the noun phrase that the tar- get noun heads, words to the left of the noun phrase, and words t...

Ngày tải lên: 23/03/2014, 18:20

... expression in WAT and BAT Quantitative measurements were then performed on whole BAT and WAT. The expression of UCP1 mRNA was measured in genital WAT, and in interscapular BAT of C57 WT and b 3 KO ... ( ANOVA <0.05)forthe main effects of group (b 3 KO vs. WT) and treatment condition (24 °Cvs.6°C 2 or 10 days) as well as for the group · treatment condition interaction...

Ngày tải lên: 23/03/2014, 20:22

... Atg13, Atg17), (b) autophago- some nucleation (Atg6, Atg14, Vps15, Vps34), (c) auto- phagosome elongation (two ubiquitin-like conjugation systems: Atg3, Atg4, Atg5, Atg7, Atg8, Atg10, Atg12, Atg16), ... shortening the lifespan. Recently, it has been shown that mutations of daf-2 or eat-2 confer resistance to these tumors [44]. These findings, together with the fact that mutations of...

Ngày tải lên: 29/03/2014, 08:20