Báo cáo khoa học: Effect of mutations at Glu160 and Val198 on the thermostability of lactate oxidase doc

... doi:10.1046/j.1432-1033.2003.03751.x lactate oxidases to investigate the effect of an E160G/V198Idouble mutation4 on thermostability and enzyme activity.Materials and methodsConstruction of the mutant lactate oxidase gene The ... in the thermal-inactivation curves of the V198I mutant and wild-type lactate oxidases (Fig. 2). Thisindicates that the V198I mutation affects the thermostability of the E160G mutant lactate oxidase, ... (corresponding to Glu160 of lactate oxidase) , and the right arrow indicates the value for Lys181 (corresponding to Val198 of lactate oxidase) .Ó FEBS 2003 Thermostability of mutant lactate oxidase 1(Eur....

Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc

... stabilize the homodimers (datanot shown). These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientation between the CD38monomers.It ... extendedexpression on the plasma membrane.Enzyme activity is not dependent on the presence of stable homodimersAs the mutations in the cytoplasmic region and the a1-helix of CD38 affected ... dependent on the formation of interdisulphide bonds between CD38monomers, indicating that the CD38 homodimers in B cellsare likely to be stabilized via noncovalent monomer–monomer interactions....

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... issues; Section 5quantitatively analyses the different contextualfeature types or feature types combination in the view of information theory and draws a series of conclusion on their predictive ... information redundancy can beused as a measure of the redundancy between the predictive information of a feature type and that of a feature type combination. Predictive Information Summation ... Data:Among the feature types mentioned above, the optimal feature type combination (i.e. the featuretype combination with the largest predictiveinformation summation) which is composed of...

Báo cáo khoa học: "Constituent-Based Morphological Parsing: A New Approach to the Problem of Word-Recognition" pdf

... Reduplication is the verbal reduplication morpheme. Of interest in this example are regressive Vowel Harmony 4, and, of course, reduplication. The input consists of the stream of segments and ... separate word n this is the case with cliticization. Also exemplary of the division of duty between the morphophonological parser and the syntactic parser is the dual status of subcategorization ... and selection of syntactic constituency. The choice between /rlu/ and /ngku/, on the other hand, is conditioned by subcategorization with respect to the prosodic 69 structure of the stem m/ngku/being...

Báo cáo khoa học: Energetic coupling along an allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA docx

... aresult of the loss of energetic contributions of alanine-substituted residues rather than the effect of such mutations on protein structure.In summary, the fact that the combined loss of freeenergy ... elaborate on these key insights into the role of cooperativity in driving protein–DNA interac-tions, we also analyzed the energetic contributions of alanine mutants in the context of binding of the ... substitution of these residues alone in the context of binding of the Jun-Fos heterodimer to DNA has little effect on the energetics of binding (Table 1). Notably, although the JunR270A mutation reduces...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

... trafﬁcking of NCT. Effect of Synoviolin on the production of AbNCT is one of the essential cofactors of the c-secretasecomplex. We therefore investigated the effect of the Synoviolin-mediated degradation ... mostlikely that the accumulation of NCT in synoviolin-nullcells is a result of the suppression of the degradation of NCT. To further investigate this, we next com-pared the degradation of NCT with ... Synovi-lin-mediated ubiquitination also regulates the trafﬁck-ing of NCT within the Golgi compartment, because the maturation of the sugar modiﬁcation of the pro-tein occurs within the Golgi compartment....

Báo cáo khoa học: Direct CIII–HﬂB interaction is responsible for the inhibition of the HﬂB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx

... [13,14].This effect generates a heat-shock response in the cell[15,16]. Maximal production of CIII prolongs the heat-induced synthesis of E. coli heat shock proteinseven at low temperature [17]. The ... proceed without ATP. Inthis respect, r32resembles kCII.Inhibition of HﬂB-mediated proteolysis of r32byCIII and by CIIIC The effect of CIII and CIIIC on the HﬂB-mediatedproteolysis of r32was ... areshown in the upper panels. Numbers on the top of each lane indi-cate the concentration of His6-CIII or CIIIC (in lM). Lane C indicates the control lane showing undigested r32. The samples...

Báo cáo khoa học: "A Feedback-Augmented Method for Detecting Errors in the Writing of Learners of English" docx

... that the effect of on the interpolated probability becomes large when the conﬁdence of the estimate of the conditionalprobability estimated from the feedback corpus issmall (that is, when there ... types of : , , and that denote the contexts consisting of the noun phrase that the tar-get noun heads, words to the left of the nounphrase, and words to its right, respectively. Then the template ... conﬁdence increases; the more conﬁdentits estimate is, the more effect it has on the inter-polated probability. Here, conﬁdence of ratiois measured by the reciprocal of variance of the ratio...

Báo cáo khoa học: b3-Adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat pptx

... expression in WAT and BATQuantitative measurements were then performed on wholeBAT and WAT. The expression of UCP1 mRNA wasmeasured in genital WAT, and in interscapular BAT of C57WT and b3KO ... (ANOVA<0.05)forthemain effects of group (b3KO vs. WT) and treatmentcondition (24 °Cvs.6°C 2 or 10 days) as well as for the group · treatment condition interaction effect, using the computer softwareSTATISTIX, ... only b-subtype controlling the emergence of brownadipocytes in WAT.Another open question is the origin and the real nature of the multilocular cells expressing UCP1 that appeared inWAT upon...

Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: ﬁne-tuning of autophagy in Caenorhabditis elegans homeostasis doc

... Atg13, Atg17), (b) autophago-some nucleation (Atg6, Atg14, Vps15, Vps34), (c) auto-phagosome elongation (two ubiquitin-like conjugationsystems: Atg3, Atg4, Atg5, Atg7, Atg8, Atg10, Atg12,Atg16), ... shortening the lifespan. Recently, it hasbeen shown that mutations of daf-2 or eat-2 conferresistance to these tumors [44]. These ﬁndings, togetherwith the fact that mutations of daf-2 or eat-2 ... empha-size the role of DAPK in the regulation of autophagy.Although several aspects of autophagy regulationhave been discovered, further examination of the vari-ous regulatory pathways is...

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