Báo cáo khoa học: Prevalent conformations and subunit exchange in the biologically active apoptin protein multimer potx

... of certain residues in the vicinity of heme. Ligand binding of human Cygb Cygb shows the characteristic absorption spectra (Fig. S2) and ligand binding for the hexacoordinated globins. The kinetics ... adopt various intermediate positions which may in uence the ligand binding kinetics. A slow relaxation process might explain the two conformations as, in the stopped-flow...

Ngày tải lên: 15/03/2014, 11:20

... are indicated by solid thin lines (b 1 ) andshadedthinlines(b 1B ), and the unidenti- fied 3¢ untranslated region of b 1B is indicated using the thin interrupted broken line. The stop codon is indicated ... microwaving the slides in Target (Dako, Carpenturia, CA, USA), the slides were placed in NaCl/P i andthenin3%H 2 O 2 ,rinsedinNaCl/P i and then the appropriate blocking...

Ngày tải lên: 16/03/2014, 23:20

... assembly. In the first model, both hydrophobic translocators exist in oligomeric form, with the major partner inserted stably into the membrane, whereas the minor protein is the link with the V antigen. ... that the needle tip, which in principle contacts all three translocon components, exists in ‘on’ and ‘off’ states [52], thus suggesting that all proteins involved...

Ngày tải lên: 14/02/2014, 22:20

... suggesting the occurrence of either a kinking or tightening of the local geometry. A similar discontinuity had been noted in solution also in the Antp HD [8,9] and the Antp (C39S) HD [10], and indeed, ... dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each 15 N– 1 H vecto...

Ngày tải lên: 18/02/2014, 16:20

... 2008 The Authors Journal compilation ª 2008 FEBS 4391 Zn-binding motif and the two disulfide bonds respec- tively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the ... bottom of the Zn-stem loop in the pRN1 prim–pol structure. How- ever, because the Zn-stem loop is a fairly self-standing structure protruding from the interface betwe...

Ngày tải lên: 18/02/2014, 18:20

... caspase-8- and caspase-9- like activities, which appear to be involved in the final resorption of the parental macronucleus during PND in T. thermophila, and suggested the involvement of mitochondria in ... for detecting the loss of membrane potential in mitochon- dria [19]. The DePsipher dye accumulates in the multimeric form in the intermembrane spaces of mito-...

Ngày tải lên: 20/02/2014, 03:20

... for relative clauses), in which both the semantics of the subtyping links themselves (normally, subset inclu- sion) and the multi-dimensionality of the empirical domain’s analysis erode into a collection ... necessar- ily be TFSs themselves, and the only method (im- plicitly) provided by the type signature to act on these values is unification. In the absence of other met...

Ngày tải lên: 20/02/2014, 15:21

... feature, the INHERITED value on the mother is the union of the IN- HERITED values on the daughters minus the TO-BIND value on the head daughter. A TO-BINDIOC feature is assigned to the cliticized ... applies, checking off the slot related to the full complement and triggering the cliticized verb form. If clitic climbing occurs as in (4a), there is ar- gument r...

Ngày tải lên: 22/02/2014, 10:20

... as the hypothesis- generating arm. Text mining With the scientific literature expanding by several thou- sand papers per week, it is obvious that no individual can read them, and there is in addition ... build- ing and intellectual environment that brings together workers from a variety of Schools at the University of Manchester focussing on Engineering and Physical Sciences, inc...

Ngày tải lên: 07/03/2014, 12:20

... 5.9. The protein gave a pink color on PAS staining (data not shown), indicating it was a gly- coprotein. The yield of purified Cyn d 24 was < 0.1% of total soluble BGP protein. Lectin-binding ... Carbohydrate staining was performed using a Glycoprotein staining kit (Pierce, Rock, IL, USA) accord- ing to the manufacturer’s instruction. Lectin-binding assays The ability of Cyn d 2...

Ngày tải lên: 07/03/2014, 12:20