Báo cáo khoa học: The CRIPTO/FRL-1/CRYPTIC (CFC) domain of human Cripto Functional and structural insights through disulfide structure analysis docx

... The CRIPTO/ FRL-1/CRYPTIC (CFC) domain of human Cripto Functional and structural insights through disulfide structure analysis Susan F. Foley, Herman W. T. van Vlijmen, Raymond E. Boynton, Heather ... Young and Dingyi Wen Biogen, Inc., Cambridge Center, Cambridge, MA, USA The disulfide structure of the CRIPTO/ FRL-1/CRYPTIC (CFC) domain of...

Ngày tải lên: 23/03/2014, 21:20

... On the basis of these results, we propose that the C-terminal domain could protect the interface between the subunits and stabilize the hexameric Hfq structure. The origin of this C-terminal domain ... change at the interface between the subunits (Fig. 2C, bottom-right), that have been described as the strand b4ofchainBandthe strand b5 of chain A in the crysta...

Ngày tải lên: 19/02/2014, 12:20

... patients. Stand- ard ratios represent the peak area of each 1mgÆmL )1 standard compared with the peak area of the internal standard. (B) Sum- mary of the range of the total bile acid con- centrations ... (internal standard). The retention (R t ) times and relative retention times (RR t ) of the bile acid standards are shown and were used as a standard to quantify...

Ngày tải lên: 23/03/2014, 10:21

... both the interaction of GM2AP with GM2 and the interaction of the GM2AP ⁄ GM2 complex with the enzyme. To delineate in detail the separate effect of the introduced muta- tions on the lipid- and the ... rates in the presence of the GM2 activator. Defects in any of the three genes encoding the polypeptides involved in GM2 degrada- tion, the a and b subu...

Ngày tải lên: 07/03/2014, 12:20

... explore the structure of the PolC N-terminal region. We found that this region includes two consecutive structural domains. Both domains are distantly related to the structure of domain V of the clamp ... test whether the removal of the second half of the PolC N-terminus would help to improve the results further. Therefore, we took only the fragment of t...

Ngày tải lên: 05/03/2014, 23:20

... first report to elu- cidate the substrate specificity of the incomplete domain and the functional relationship of tandemly repeated domains in BPPs. We conjecture that dual -domain BPPs have succeeded ... pH (6.0–8.0) and at low temperatures (0–35 °C). We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of...

Ngày tải lên: 14/02/2014, 15:20

... [20]. On the other hand, the activity of the purified recombinant C153S mutant was similar to that of the wild-type protein (data not shown), and the kinetics of inactivation in the presence of 1 ... A 4 -GAPDH and on native spinach A 2 B 2 -GAPDH and A 8 B 8 -GAP- DH. We examined the effect of these modifications on the enzyme activities, their reversibility in...

Ngày tải lên: 19/02/2014, 05:20

... (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent weight of GlcN [23]. Circular ... with 200 mg KBr and 40 mg of the mixture was pelletized. The reproducibility of the spectra was verified on two preparations and the degree of acet...

Ngày tải lên: 19/02/2014, 12:20

... influenced by the frequency of graphemic units in the language and by the predictability of their mapping to phonemes. We argue that these results implicate the availability of graded knowledge of grapheme-phoneme ... These findings are incompatible with current implementations of the dual-route theory (Coltheart et aL, 1993). The "central dogma" of...

Ngày tải lên: 20/02/2014, 19:20

... scheme of the secondary structure of the 123 residue chain of a-LA [4]. The four a-helices (H1–H4) are indicated by large boxes, and the corresponding chain segments are given above them. The three b-strands ... clus- tered at the level of helical segments A, C and D of the native protein, whereas the central region of the protein contains many negatively c...

Ngày tải lên: 06/03/2014, 09:22