Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I ppt

... digested with SpeI and NsiI and cloned in the vector pLB85 digested with XbaI and PstI. Following ligation the plasmids were intro- duced directly to L. lactis LB436, carrying plasmid pLB65 in ... were digested with XhoI ⁄ BamHI and BamHI ⁄ XbaI, respectively, and cloned in iden- tical restriction sites in plasmid pG + host8, using E. coli KW1 as cloning host. The resulting...

Ngày tải lên: 19/02/2014, 17:20

... the triple mutant was func- tionally deficient in b2-microglobulin interactions and incapable of regulating transferrin binding, once again, reminiscent of the HFE-C282Y variant. Single and double ... of N-glycosylation sites in the bovine cation-dependent mannose 6-phosphate receptor: effects on ligand binding, intracellular targetting and association with binding immunoglob...

Ngày tải lên: 06/03/2014, 22:21

... Differ- ential involvement of calmodulin-dependent protein kinase II-activated AP-1 and c-Jun N-terminal kinase- activated EGR-1 signaling pathway in tumor necrosis factor-ƒ and lipopolysaccharide-induced ... experiments of competitive receptor-binding assays using isolated cuticles and 125 I- labeled GBP were carried out, but we did not find any significant difference in the lig...

Ngày tải lên: 16/03/2014, 11:20

... considered as major players in RNA folding and in the assembly and functioning of RNP machines, mostly through transient interactions with the RNA. Transient RNA–protein interactions in RNA folding ... promoting folding via unfolding or via annealing acceleration; and (c) RNA helicases, which accelerate the unwinding of many RNAs under conditions of ATP binding and hyd...

Ngày tải lên: 14/02/2014, 19:20

... both in vivo and in vitro [23,34] (Fig. 2E,F), and (b) H–D exchange experiments, which identified residues in helices 1 and 2 as involved in intersubunit interactions in mature HIV-1 capsid-like ... pressure to maintain an optimum balance between capsid stability (i. e. for structural integrity in the virion) and instability (i. e. for viral core disintegration and RNA...

Ngày tải lên: 18/02/2014, 06:20

... FEBS reaction, to generate the correct conformation of pro- line, which is a rate-limiting step in protein folding. This ability possibly in uences the role of these enzymes in signaling, RNA splicing, ... overexpression of exogenous tRNA Lys3 resulted in higher incorporation into virions, increased tRNA annealing to viral RNA and greater infectivity of the virus. The absence...

Ngày tải lên: 07/03/2014, 00:20

... bind- ing domain is mutated to arginine, still interacted with PTI1-4. These data indicate that the kinase activity of OXI1 is not required for the interaction with PTI1-4. OXI1 interacts with PTI1-4 ... haemagglutinin; HIS, histidine; HR, hypersensitive response; MAPK, mitogen-activated protein kinase; MAPKK, mitogen-activated protein kinase kinase; MBP, myelin basic protein; OXI1, oxidati...

Ngày tải lên: 14/02/2014, 19:20

... USA) (although it is in Phase II trials) is not discussed because its mechanism of action is not based on inhibi- tion of CA self-assembly. Bevirimat is a novel HIV-1 maturation inhibitor that inhibits ... dimer- ization interface is fully contained within the isolated CTD. This observation has facilitated the initial search for inhibitors of HIV-1 assembly by using simplified experime...

Ngày tải lên: 18/02/2014, 06:20

... that, in addition to utilizing a higher number of binding sites, mutants II and III gained their modest (approximately ten-fold) mem- brane binding affinity enhancement by minor increases in the ... mutants II and III each have approximately 50%, whereas wild-type protein C has obtained a mere 19% of its potential binding. This indicates that the mutations introduced in mutants I...

Ngày tải lên: 18/02/2014, 06:20

... family, is important in the regulation of growth, differentiation and survival of various cell types. Ligand binding to EGFR results in receptor dimerization, activation of its tyrosine kinase and phosphorylation ... EGFR-tyrosine kinase is a promising therapeutic target. Although in vitro and in vivo studies have shown the anti-tumor activity of EGFR-tyrosine kinase inh...

Ngày tải lên: 18/02/2014, 13:20