Báo cáo khoa học: Alternative binding modes of an inhibitor to two different kinases doc

... Alternative binding modes of an inhibitor to two different kinases Erika De Moliner*, Nick R. Brown and Louise N. Johnson Laboratory of Molecular Biophysics, Department of Biochemistry, ... designed to target one particular kinase with other kinases. It is generally assumed that the mode of binding of an inhibitor to one kinase is likely to be similar...

Ngày tải lên: 23/03/2014, 21:20

... recombinant expression. With four structurally variable loops, the binding site of lipoca- lins is less complex and easier to manipulate [5] than the CDR of antibodies, which is composed of alto- gether ... dubbed ‘anticalins’ [7,8]. Properties and potential of anticalins Engineered lipocalins offer several advantages over immunoglobulins. Their size, of < 20 kDa, is much sma...

Ngày tải lên: 23/03/2014, 07:20

... deactivation to the originally high basal activation state in a dose-depen- dent manner, and thus acts as an inverse antagonist of ERRc. ERRs are a subfamily of orphan NRs and are clo- sely related to two ... role of Arg316 for the ERRc receptor in ligand binding. This kind of structure–activity relationship between NRs and ligands has never been explored, and thus it is very...

Ngày tải lên: 18/02/2014, 16:20

... 8673–8677. 62 Friedman KJ, Kole J, Cohn JA, Knowles MR, Silverman LM & Kole R (1999) Correction of aberrant splicing of the cystic fibrosis transmembrane conduc- tance regulator (CFTR) gene by antisense ... & Bojar H (2002) Analysis of BRCA1, TP53, and TSG101 germline mutations in German breast and ⁄ or ovarian cancer families. Cancer Genet Cytogenet 138, 120–127. 84 Lee WI, Torg...

Ngày tải lên: 06/03/2014, 09:22

... 4A) [19,24–26]. Binding of SR proteins downstream of a splice acceptor can increase the efficiency of U2AF binding to the PPT, either by displacement of hnRNP A1 protein that blocks access of spliceosomal ... multimeriza- tion of hnRNP A1 towards the A3 3¢ss leads to the occlusion of the PPT and to site A3 inhibition [36]. Enzymatic and chemical probing revealed the occ...

Ngày tải lên: 06/03/2014, 09:22

... metal ion binding. Ca 2+ and Mg 2+ binding lead to an increase in the fluorescence intensity of the trypto- phans in an ion-dependent fashion. Ca 2+ binding leads to a two- fold increase in tryptophan ... hand, behave differently, and seem to have specific affinity for only one of the binding sites. Mg 2+ binding reduces the intensities of only the c-band, and does not...

Ngày tải lên: 07/03/2014, 00:20

... coupled binding and folding mecha- nism is likely to be relevant in vivo. This binding mech- anism is assumed to be generally important for transcription factor interactions with other proteins and ... activation by galactose To determine whether the activator -binding domains of the SWI ⁄ SNF complex are important for its recruit- ment to promoters in vivo and to determine w...

Ngày tải lên: 07/03/2014, 00:20

... responding to diverse extracellu- lar signals? Analysis of the sensor kinases and response regulators of Streptomyces coelicolor A3(2). Microbio- logy 150, 2795–2806. 10 Brian P, Riggle PJ, Santos RA ... [10,11], and the VanR–VanS system activates the expression of vancomycin resistance [12,13]. Phosphate control of the production of actino- rhodin and undecylprodigiosin in S. l...

Ngày tải lên: 07/03/2014, 10:20

... subdomain of the first (N-terminal) of fimbrin’s two ABDs [20]. In light of the extensive structural resemblance of fimbrin and plectin ABDs it was therefore of interest to assess IF -binding activity of ... binding domain of plectin suggests alternative mechanisms for binding to F-actin and integrin beta 4. Structure 11, 615–625. 25. Hanein, D., Volkmann, N., Goldsmit...

Ngày tải lên: 07/03/2014, 15:20

... RG (2005) Antiapoptotic and anti-inflammatory mechanisms of heat-shock protein protection. Ann N Y Acad Sci 1053, 74–83. 34 Liang P, Jiang B, Yang X, Xiao X, Huang X, Long J, Zhang P, Zhang M, Xiao ... inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells Bimei Jiang 1 , Kangkai Wang 1 , Pengfei Liang 2 , Weimin Xiao 1 , Haiyun W...

Ngày tải lên: 16/03/2014, 01:20