Báo cáo khoa học: Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase docx

... concentration of enolase. Ó FEBS 2003 Enolase from Trypanosoma brucei (Eur. J. Biochem. 270) 3207 Alignment of sequences and structure modelling of T. brucei enolase A sequence alignment of T. brucei enolase ... Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase Ve ´ ronique Hannaert 1 , M...

Ngày tải lên: 23/03/2014, 21:20

... constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement of rate constant of substrate-cleavage ... Tinoco I Jr (1982) Comparison of the kinetics of ribooligonucleotide, deoxyribooligonucleo- tide, and hybrid oligonucleotide double-strand forma- tion by temp...

Ngày tải lên: 18/02/2014, 18:20

... concentration of 2 mm) and the kinetic parameters of recombinant EhMGL1 and EhMGL2 (Tables 1 and 2). Despite the heterogeneity of the EhMGL1 preparation used in the previous study [13], the kinetic ... Moreover, the effects of the Y108F substitution on the K m and k cat of EhMGL1 for TFM are opposite to those of Y111F of EhMGL2; the K m and k cat of EhMGL1(Y108F)...

Ngày tải lên: 07/03/2014, 05:20

... Proki ´ c Alfa-Informatica University of Groningen The Netherlands j.prokic@rug.nl Abstract The aim of this paper is to present a new method for identifying linguistic structure in the aggregate analysis of the language ... techniques and dialectometry itself. Two at- tempts to overcome this drawback are presented in Nerbonne (2005) and Nerbonne (2006). In both of these papers t...

Ngày tải lên: 20/02/2014, 12:20

... and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature 381, 335–341. 25 Suzuki M, Youle RJ & Tjandra N (2000) Structure of bax. Coregulation of dimer formation and ... with a reduction in turn and unordered random- coil structures, but especially at the expense of the b-strand structures, which are practically absent in the presence of the l...

Ngày tải lên: 19/02/2014, 07:20

... allow evaluation of the T m . Charged states of Arg and ArgEE In order to understand the importance of amphiphilicity, titration curves of Arg and ArgEE were compared (Fig. 5 A). The pK a of amino groups ... inactivation and aggregation of l ysozyme [18]. These data s how that ArgE E is a new candidate additive for the prevention of thermal inactivation of lysozyme. pH d...

Ngày tải lên: 19/02/2014, 16:20

... minireview will focus on the cellular and molecular biology of PAI-2 and highlight some of the most recent findings on the role and impressive pattern of regulation of this enigmatic protease inhi- bitor. ... infection Cancer A number of in vivo studies have assessed the prognos- tic relevance of tumour- and stromal-derived PAI-2 in the metastatic spread of cancer of...

Ngày tải lên: 20/02/2014, 02:21

... S, Rothwell NJ, Tyrell PJ, Hopkins SJ & Rowland M (2008) Pharmacokinetic modelling of interleukin-1 receptor antagonist in plasma and cerebrospinal fluid of patients following subarachnoid haemorrhage. ... Brain Research and Department of Neuroscience, ‘‘Tor Vergata’’ University, Rome, Italy 4 University Centre for Adaptive Disorders and Headache, Section of Neuropharmacolo...

Ngày tải lên: 07/03/2014, 03:20

... systematically studies the s ubstrates of hKAT-I and reassesses t he effects of pH, Tris, amino acids and a-keto acids on the a ctivity of t he enzyme. T he experi- ments were comprised of functional ... as lmolÆmin )1 Æmg )1 . Effect of buffer and pH on hKAT-I. To determine the effect of pH on hKAT-I activity, a buffer mixture consisting of 100 m M phosphate and 100 m M...

Ngày tải lên: 07/03/2014, 16:20

... understanding of what the purpose of the analysis is. As we have stressed repeatedly, the purpose of a systems biology problem is generally differ- ent from that of a classical engineering and statistical problem, ... the choices regarding which model structures to consider as different cases of a super model structure (contain- ing all of them as special cases), and which m...

Ngày tải lên: 23/03/2014, 06:20