Báo cáo khoa học: Membrane targeting of a folded and cofactor-containing protein potx

... RRﬁKK exchange was achieved using theprimers 5¢-CATCACTTTGACAGCGTCTTTCTTGCTCTTGCTGATTGGCTTATCG-3¢ and 5¢-CGATAAGCCAATCAGCAAGAGCAAGAAAGACGCTGTCAAAGTGATG-3¢ using the QuikChange kit (Stratagene) ... signal peptide of preHiPIPwas mutated to KK using the primer couples 5¢-AAGAGCAAGAAAGACGCTGTCAAAGTGATG-3¢/5¢-TCCGGATATAGTTCCTCCT-3¢ and 5¢-ACGTTACTGGTTTCACATTC-3¢/5¢-AGCGTCTTTCTTGCTCTTGCTGATTGGCTT-3¢ ... 1221 Membrane targeting of a folded and cofactor-containing protein Thomas Bru¨ ser1, Takahiro Yano2, Daniel C. Brune3 and Fevzi Daldal1,21Department of Biology, University of Pennsylvania,...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... Anandatheerthavarada and Narayan G. AvadhaniDepartment of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USACytochrome P450s (CYPs) belong to a ... Anandatheerthavarada HK,Addya S, Pandak W & Avadhani NG (1999) Dual targeting property of the N-terminal signal sequence of P450 1A1 . Targeting of heterologous proteins toendoplasmic reticulum and ... Dasari VR, Anandatheerthavarada HK, Robin MA,Boopathi E, Biswas G, Fang JK, Nebert DW &Avadhani NG (2006) Role of protein kinase C-mediated protein phosphorylation in mitochondrial translocationof...

Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... plasma membrane potential and thenbe further accumulated within the mitochondria due to themitochondrial membrane potential [11]. From the knownplasma and mitochondrial membrane potentials and ... alkyla-tion leading to a depletion of mtDNA in intact cells(Fig. 1). Here we report the synthesis and characterization of a novel mitochondria-targeted alkylating reagent and show that it alkylates ... mMEDTA and 1 lgwasrunat60 Vona0.6%agarose/ethidium bromide gel. The DNA was electrotrans-ferred to a positive nylon membrane (Hybond N+,Amersham Pharmacia Biotech), treated with 0.4MNaOH,and...

Báo cáo khóa học: Membrane transport of fatty acylcarnitine and free L-carnitine by rat liver microsomes pot

... enzymes such as DGAT, ACAT and AEAT are generated by a malonyl-CoA-insensitivecarnitine acyltransferase (CAT) that is localized in the ERlumen [18,19]. It has been envisaged that the substrate forthis ... fatty acyl-CoAsubstrate for AEAT, DGAT and ACAT in the ER lumen.Ó FEBS 2004 Transport of carnitine and acylcarnitine (Eur. J. Biochem. 271) 955we found that alamethicin abolished latency of ... Waterman, I.J. & Zammit, V .A. (2002) Diﬀerential eﬀects of fenoﬁbrate or simvastatin treatment of rats on hepatic microsomalovert and latent diacylglycerol acyltransferase activities. Diabetes51,...

Báo cáo khoa học: "Unsupervised Learning of Narrative Schemas and their Participants" potx

... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 602–610,Suntec, Singapore, 2-7 August 2009.c2009 ACL and AFNLPUnsupervised Learning of Narrative Schemas and ... narrative schema model.2.1 Narrative Event ChainsNarrative Event Chains are partially ordered sets of events that all involve the same shared par-ticipant, the protagonist (Chambers and Jurafsky,2008). ... arguments. An event slot is a tuple of an event and a particular argument slot (grammatical rela-tion), represented as a pair v, d where v is a verb and d ∈ {subject, object, prep}. A chain is a tu-ple...

Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

... 213–222.66 Hamaguchi M, Hamada D, Suzuki KN, Sakata I &Yanagihara I (2008) Molecular basis of actin reorgani-zation promoted by binding of enterohaemorrhagicEscherichia coli EspB to alpha-catenin. ... Y, Sagara H, Kabe Y, Azuma M, Kume K,Ogawa M, Nagai T, Gillespie PG, Sasakawa C &Handa H (2007) The enteropathogenic E. coli effectorEspB facilitates microvillus effacing and antiphago-cytosis ... Shibagaki Y, Danbara H & Okada N (2010)Functional characterization of SsaE, a novel chaper-one protein of the type III secretion system encodedby Salmonella pathogenicity island 2. J Bacteriol...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... Structure and function of a regulated archaealtriosephosphate isomerase adapted to high temperature.J Mol Biol 342, 861–875.12 Gayathri P, Banerjee M, Vijayalakshmi A, Azeez S,Balaram H, Balaram ... inenzymes: a study of triosephosphate isomerase and comparison with methyl glyoxalsynthase. Adv Protein Chem 66, 315–372.45 Gunasekaran K, Ramakrishnan C & Balaram P (1996)Disallowed Ramachandran ... mutationsMousumi Banerjee1, Hemalatha Balaram2 and Padmanabhan Balaram11 Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India2 Molecular Biology and Genetics Unit, Jawaharlal...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... M, Hasegawa K, HoritaC & Akera S (1999) A new matrix protein familyrelated to the nacreous layer formation of Pinctadafucata. FEBS Lett 462, 225–229.5 Kono M, Hayashi N & Samata T ... byinvertebrates, and has three crystal phases: calcite, ara-gonite and vaterite. Although calcite is the most stablecrystal thermodynamically, many organisms can formmetastable aragonite crystals ... region wasdecreased and a new band was detected at 64 kDa, butonly after treatment with heparitinase II (Fig. 5A) .Although the same band was obtained after digestionFig. 3.45Ca overlay analysis...

Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx

... absorption bands. Heme catabolism byHOs of mammals, pathogenic bacteria, cyanobacteria and probably insects is considered to have a similarmechanism, because the characteristic absorptionbands of verdoheme ... Then, a broad bandappeared at around 660 nm, and was maximal 9–12 minafter initiation of the reaction. The spectral features of the ﬁnal reaction mixture were analogous, but not iden-tical, ... T, Zhang X, Sun D,Sato M, Sasahara M, Kayama T, Ikeda-Saito M &Yoshida T (2000) Histidine 20, the crucial proximalaxial heme ligand of bacterial heme oxygenase Hmu Ofrom Corynebacterium...

Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf

... smaller compound PEA(Fig. 1B) and a water molecule are present and PEX and PEA reﬁned satisfactorily with occupancies of 0.7Table 1. Data and reﬁnement statistics. Values in parentheses pertain ... 25% of the accessiblesurface area of a monomer contributes to LlPDHdimer formation. Whereas LlPDH and OaPDH have a buried surface area of  5500 A ˚2, TbPDH has a largerinterface surface area ... suite: programs for protein crystallo-graphy.Acta Crystallogr D Biol Crystallogr 50, 760–763.28 Navaza J (1994) Amore – an automated package formolecular replacement. Acta Crystallogr A 50, 157–163.29...

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