Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx

... nitro- gen just before use. When required, protein samples were purged with nitrogen before storage or transfer. Standard recombinant DNA techniques were followed as described elsewhere [58]. Protein ... secondary antibodies only. The labels mentioned on the x-axis denote the respective WhiB proteins used as antigen in each case. All experiments were performed in triplicate and the results...

Ngày tải lên: 18/02/2014, 12:20

... inhibited the degradation of a2M by cathepsin E. These results indicate that the presence of Ala in the P¢2 is essential for selective cleavage the synthetic peptides by cathepsin E. The presence of ... in a2M by cathepsin E, we synthesized some peptides corresponding to part of the cleavage site and analyzed the susceptibility of these peptides to cleavage by ca...

Ngày tải lên: 23/03/2014, 21:20

... achievable by grafting two different functional peptide sequences. Recently, several pep- tides with affinity for inorganic material surfaces have been selected from a peptide library, and the replace- ment ... and randomized sequence lengths without decreasing density. Detailed tertiary structural analyses of protein–protein complexes allow accurate description of epitope locati...

Ngày tải lên: 06/03/2014, 11:20

... would extend the a-helix and penetrate into the membrane, forming the pore. However, the molecular bases directing these processes are still largely unknown. In this regard, mutagenesis studies ... However, when only the regular secondary elements were considered these values dropped to 0.7 and 0.6 A ˚ , respectively, showing that these regions, which constitute the protein core, are simi...

Ngày tải lên: 14/03/2014, 23:20

... One of the interesting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9]. This sequence of amino acids, which is missing in the sequence of bacterial ... that define pantoate in site I and site II are clearly visible. The atoms of the pantoate molecule are labeled. The electron density clearly defines the backbones and side chains...

Ngày tải lên: 16/02/2014, 09:20

... contain the enzyme. The fluorescence intensity changes where known concentrations of the substrate were completely degraded by the addition of an excess amount of the collagenase under these assay ... the values of three independent experiments is shown with standard errors. (C) The pH-depend- ence of the ligation process. Upper panel shows SDS ⁄ PAGE analysis of the pH-dependenc...

Ngày tải lên: 19/02/2014, 07:20

... abscissa. The measurements were performed at 15 °C. The results were expressed as a percentage of the ATPase activity obtained in the absence of TnI. Each point is an average of three determinations. (A) ... the presence or absence of Ca 2+ .The pellets (P) and supernatants (S) were redissolved in equivalent volumes of 5 M urea solution and then run on SDS ⁄ PAGE. Lanes a and...

Ngày tải lên: 20/02/2014, 01:20

... to the expression of novel splicing isoforms and ⁄ or loss of an existing one. This phenomenon can either generate new substrates for evolution or cause genetic disease when aberrant isoforms altering ... splice sites have rarely been taken into consideration or even reported owing to technical difficulties in sequencing entire genes (also until recently an expensive task) and in the u...

Ngày tải lên: 06/03/2014, 09:22

... the more accumulated hydration heat the higher hydration temperature. And the temperature differences between the center and surface of the member were 14.3 and 20.6℃ at the highest temperature. ... temperature differences between the center and surface of mat foundation and then to minimize the highest temperature of lower level, accelerating hydration heat rise of upper...

Ngày tải lên: 07/03/2014, 03:20

... when current flow reached steady state. The current evoked by PAT2 at a given m embrane poten tial was calculated as the difference between the c urrents measured i n the presence and the absence ... ensure high enough inward currents even at lower expression level. Substrate-evoked currents w ere t ransformed according to Eadie–Hofstee and after linear regression the apparent substrate...

Ngày tải lên: 07/03/2014, 16:20