Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx
Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... nitro-
gen just before use. When required, protein samples were
purged with nitrogen before storage or transfer. Standard
recombinant DNA techniques were followed as described
elsewhere [58].
Protein ... secondary antibodies only. The labels mentioned
on the x-axis denote the respective WhiB proteins used as antigen
in each case. All experiments were performed in triplicate and the
results...
Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx
... inhibited the
degradation of a2M by cathepsin E. These results indicate
that the presence of Ala in the P¢2 is essential for selective
cleavage the synthetic peptides by cathepsin E. The
presence of ... in a2M by cathepsin
E, we synthesized some peptides corresponding to part of
the cleavage site and analyzed the susceptibility of these
peptides to cleavage by ca...
Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... achievable by grafting two different
functional peptide sequences. Recently, several pep-
tides with affinity for inorganic material surfaces have
been selected from a peptide library, and the replace-
ment ... and randomized sequence lengths without
decreasing density. Detailed tertiary structural analyses of protein–protein
complexes allow accurate description of epitope locati...
Báo cáo khoa học: Intrinsic local disorder and a network of charge–charge interactions are key to actinoporin membrane disruption and cytotoxicity ppt
... would extend the a-helix
and penetrate into the membrane, forming the pore.
However, the molecular bases directing these processes
are still largely unknown. In this regard, mutagenesis
studies ... However, when
only the regular secondary elements were considered
these values dropped to 0.7 and 0.6 A
˚
, respectively,
showing that these regions, which constitute the protein
core, are simi...
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... One of the
interesting features of the plant PSs is the presence of
a conserved 24 residue insertion in the sequence [9].
This sequence of amino acids, which is missing in the
sequence of bacterial ... that define pantoate in site I and site II are clearly visible. The atoms of the pantoate
molecule are labeled. The electron density clearly defines the backbones and side chains...
Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... contain the enzyme. The fluorescence intensity
changes where known concentrations of the substrate were
completely degraded by the addition of an excess amount
of the collagenase under these assay ... the values of three independent experiments is shown with standard errors. (C) The pH-depend-
ence of the ligation process. Upper panel shows SDS ⁄ PAGE analysis of the pH-dependenc...
Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... abscissa.
The measurements were performed at 15 °C. The results were
expressed as a percentage of the ATPase activity obtained in the
absence of TnI. Each point is an average of three determinations.
(A) ... the presence or absence of Ca
2+
.The
pellets (P) and supernatants (S) were redissolved in equivalent volumes of 5
M urea solution and then run on SDS ⁄ PAGE. Lanes a and...
Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf
... to
the expression of novel splicing isoforms and ⁄ or loss of an existing one.
This phenomenon can either generate new substrates for evolution or cause
genetic disease when aberrant isoforms altering ... splice sites have rarely been
taken into consideration or even reported owing to
technical difficulties in sequencing entire genes (also
until recently an expensive task) and in the u...
Báo cáo khoa học "ADIABATIC TEMPERATURE RISE AND REACTION RATE OF MASS STRUCTURE IN LOTTE CENTER HANOI PROJECT " pptx
... the more
accumulated hydration heat the higher hydration temperature. And the temperature differences between the
center and surface of the member were 14.3 and 20.6℃ at the highest temperature. ... temperature differences between the center and surface of mat
foundation and then to minimize the highest temperature of lower level, accelerating hydration heat rise of upper...
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... when current
flow reached steady state. The current evoked by PAT2 at a
given m embrane poten tial was calculated as the difference
between the c urrents measured i n the presence and the
absence ... ensure
high enough inward currents even at lower expression level.
Substrate-evoked currents w ere t ransformed according to
Eadie–Hofstee and after linear regression the apparent
substrate...
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