Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
... of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 Robert D. Finn 1 , Jaswir Basran 2 , Olivier Roitel 2 , C. Roland Wolf 1 , ... and kinetic properties of NR1, and as we have shown for CPR and P450 BM3 (23,24,28) the properties of the flavin binding domains of...
Ngày tải lên: 23/03/2014, 21:20
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... Le a determinant. As the C 3a ion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminal GlcNAc. The position of the second Fuc 3-linked to the internal ... demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosacchar...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx
... strand DNA due to the unpaired 3¢-end of the primer, until the ÔGGÕ bases in the hairpin stem are freed by elevating the reaction temperature and paired with the ÔCCÕ bases at the 3¢-end of primer ... that the failure of the primer 2–DNA template hybrid primer extension reaction was due to the effects of the hairpin structure on the process of primer 2 anne...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot
... Possible roles of the cation include the activation of water for the hydration reaction and ⁄ or the stabilization of the anion transition state. In the latter case, if the sub- strate coordination to the ... the mechanism, the substrate binding site and the role of the metal cofactor in the enzyme. Results Cloning, expression and purification of BphH...
Ngày tải lên: 30/03/2014, 15:20
Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... in the test and refer- ence samples. The presence of the internal standard probes at different locations of the array allowed quantification of the local background and evaluation of the array homoge- neity, ... into account in the normalization. Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was performed on...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... is the concentration of the protein–lig- and complex, and [P] and [L] are the concentrations of the free protein and free ligand (i.e. inhibitor), respect- ively [68]. Expressed in terms of r, ... r as a function of the free ligand concentration and the fit to Eqn (3) (solid line), obtained in the case of TRX I and S-Xyl5-Me. On the basis of the K a ob...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... identification of the func- tional domains of accessory factors HNF3 beta (hepatic nuclear factor-3 beta) and HNF4 and the necessity of proper alignment of their cognate binding sites. Mol Endocrinol ... the respective indicated times. Cells in the absence of any of these were taken as the control (Con). Two micrograms of total RNA were reverse transcribed with ra...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx
... at- tached to words. The tags indicate the inflectional and derivational properties of the word and the word class membership, the tags attached during the last stage of the analysis indicate ... functions. The underlying principle in determin- ing both the morphological interpretation and the syntactic functions is the same: first all the pos- sible...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
... lectins in the case of the siglecs [72], was noted to affect binding properties. Compared with the solid-phase assays, the studies of cell binding allowed determination of the ligand properties of biantennary N-glycans ... of the introduction of the substitu- tion. The results, expressed as median fluorescence intensity and percentage of positive cells, refl...
Ngày tải lên: 30/03/2014, 13:20
Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx
... zinc and MT protect the liver and the heart against the toxic effects of ethanol [45–47]. The above results suggest that a critical aspect of the protective function of MT is the scavenging of the ... none of the aldehydes affects the absorbance of PAR. The data for the reaction of MT with formalde- hyde were corrected for the absorbance changes in t...
Ngày tải lên: 19/02/2014, 06:20