Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot

... 2003 Artemin and ferritin from Artemia (Eur. J. Biochem. 270) 141 Developmental regulation of artemin and Artemia ferritin mRNAs Probing of Northern blots with a labeled artemin probe revealed bands ... vertebrates, which contains ferritin H from fish and amphibians, and a third for invertebrates in which Artemia ferritin and artemin reside (Fig. 6). Ar...

Ngày tải lên: 23/03/2014, 20:22

... stained particles of artemin indicated the lack of metal storage capacity. Artemin, apoferritin and ferritin inhibit citrate synthase denaturation Artemin, apoferritin and ferritin protected ... primary structure of artemin from Artemia cysts. Eur J Biochem 193, 737–750. 16 Chen T, Amons R, Clegg JS, Warner AH & MacRae TH (2003) Molecular characterization of...

Ngày tải lên: 16/03/2014, 11:20

... PUF-8, Drosophila and human Pumilio and yeast Puf3 recog- AB CD Fig. 6. Analysis of binding affinity between APUM-2 and the FAS-2 3¢ UTR transcripts with nucleotide substitutions upstream and down- stream of ... that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or turnover. A la...

Ngày tải lên: 18/02/2014, 06:20

... residues instead of 15 residues, including strand S2, between helices H1 and H2, and 84 residues instead of 10 resi- dues between helices H2 and H3 (Fig. 2). Molecular characterization of zebrafish ... entry and routing of PrP Sc in the early steps of infection. Our observations of a prp2 hybridization signal in the posterior intestine of zebra- fish larvae call for ev...

Ngày tải lên: 19/02/2014, 16:20

... Munn 2,3 and Hongyuan Yang 1 1 Department of Biochemistry, Faculty of Medicine, National University of Singapore, Republic of Singapore 2 Institute of Molecular and Cell Biology, A*STAR Biomedical ... Institutes, Singapore, Republic of Singapore 3 Institute for Molecular Bioscience, University of Queensland, St Lucia, Queensland, Australia The oxysterol binding protein (...

Ngày tải lên: 07/03/2014, 21:20

... the presence of FMN instead of FAD, or NADPH instead of NADH no NoxC activity was found For this reason, further purification and characterization of NoxC was abandoned. Purification of the recombinant ... between 71% and 95% of the amount Fig. 3. SDS/PAGE of extracts of recombinant E. coli containing NoxA-1, NoxB-1 or NoxC from A. fulgidus. M, Calibration proteins. The...

Ngày tải lên: 08/03/2014, 02:21

... (lanes 1–3 and 7–9) and nuclear fractions (lanes 4–6 and 10–12) we re prepared from 8-h-old early b lastulae (lanes 1, 4, 7, and 10), 12-h-old mid-blastulae (lanes 2, 5, 8, and 11), and 24-h-old ... kit (Amersham Pharmacia Biotech) with TG5 and TG3V primers. Isolation of cDNA clones and DNA sequencing Adaptor-ligated double stranded cDNA was prepared from poly(A) + RNA...

Ngày tải lên: 08/03/2014, 10:20

... season of H. longicornis and body tempera- ture of its host. In general, the feeding activity of ticks occurs from spring through summer, and the body temperature of hosts such as cattle, dogs and ... a molecular mass of 53 kDa and a pI of 7.5 (Fig. 2A), corresponding to the predicted size of the putative pro- tein calculated from the HlSCP1 amino acid sequence. To...

Ngày tải lên: 16/03/2014, 10:20

... previo usly described [11]. PKG and cGMP were purchased from Promega; MAPK, CaMKII, and calmodulin from Upstate; and CK1, CK2, and Cdk1 from New England Biolabs. Cdk5 and p25 were coexpressed in ... estriction and DNA modifying enzymes were from New England Biolabs. Electrocompe- tent bacteria were from Life Technologies, Inc. Cloning and expression vectors were from...

Ngày tải lên: 16/03/2014, 18:20

... ESAT-6– CFP-10 and Rv0287–Rv0288 could result from the dif- ference in the folding state of Rv3620c from that of CFP-10 and Rv0287. Thermal unfolding of proteins The presence of any stable tertiary ... unfolding of the proteins is required for dissociation of the complex and membrane binding. In the presence of membrane mimetics, the a-helical contents of Rv3619c an...

Ngày tải lên: 22/03/2014, 16:21