Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx

... Characterization of native and recombinant A 4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 Emmanuelle ... analysis of native GAPDH shows that it is a complex of GAPDH and the small protein CP12. In vitro reconstitution assays indicate that the kinetic...

Ngày tải lên: 23/03/2014, 20:22

... dechloratans, and the recombinant version of the protein produced in Escherichia coli, suggests the presence of a covalent modification in the native enzyme. Although t he native and r ecombinant N- and ... in Fig. 1) in the e lectropherogram of the n ative enzyme are missing in the electropherogram of the recombinant enzyme. There are also two peaks in...

Ngày tải lên: 23/03/2014, 12:20

... comparison of the residues serving as the ligand for the M3 metal ion is shown in Table 1. The residues of the direct ligands to the metal were the same as in hPLAP, but those of indirect ligands, which ... we used the swiss-model program to construct 3D models of rIAP-I and rIAP-II; we then analyzed the active site of the enzymes and studied the effect...

Ngày tải lên: 07/03/2014, 17:20

... containing ampicillin, and 0.5 mL of the culture was then inoculated into 50 mL of the same medium. A crude protein extract containing GST–mSPS1 and His–mDLP1 (or hDPS1 and hDLP1) was then prepared from the transformant ... nos BF180140 for the murine homolog and AI590245 and AI261617 for the human homolog) were selected. We also cloned the cDNAs with the h...

Ngày tải lên: 16/03/2014, 23:20

... abolished the rotenone-induced inhibition of R phases. Taken together, these findings indicate that the activity of both respiratory chain and ATP synthase were required for the recovery of the mitochondrial potential. ... that the activity of both respiratory chain and ATP syn- thase is required to activate the R phase. On the other hand, the frequency of D pha...

Ngày tải lên: 30/03/2014, 16:20

... more weakly with the reagents currently available. To analyse further the composition of the PS1 complex, samples with high expression of PS1 complex were selected for further study. BN/PAGE gel bands ... to the generation of Ab peptides in pathological processes. Study of the characteristics of the PS complex will facilitate elaboration of the molecular proces...

Ngày tải lên: 16/03/2014, 16:20

... (i.e. the development of the gills and the production of an adult shell), which is accom- panied by significant growth of the soft body parts [21]. The mantle edge governs shell formation and body ... Metamorphosis represents the ulti- mate stage of oyster development, and is characterized by the degeneration of larval tissues, such as the velum and the foot...

Ngày tải lên: 19/02/2014, 00:20

... and quantitative real-time RT-PCR, the locations of the primers DN)105 and DN)9 used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... fragments F and I. The number at the right of each RT-PCR product represents the length of the prod- uct. The scheme also shows the location of the r...

Ngày tải lên: 19/02/2014, 06:20

... but further enhances the activity of the enzyme whose C-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. ... XL10 and the frame and the identity of the cloned fragment verified by sequencing. The construct with the N-terminal portion of PPI1 has been previously descri...

Ngày tải lên: 19/02/2014, 07:20

... primers. Both of the genes were constructed either with or without a His-tag at the 3¢ terminus of the coding region. Thus, the stop codon of SNO1 and SNZ1 was replaced with bases coding for the sequence ... in the presence of 1–10 m M glutamine and 30 lgof the complex at 30 °C for 10 min. The sample was then boiledfor1minandkeptfrozenat)80 °C for subsequ...

Ngày tải lên: 19/02/2014, 12:20