... incorporation [20]. In contrast,coordination structures of the L99T, L99F, and L115Tmutants were similar to that of the wild-type enzyme.We further found that the rates of auto-oxidation and the ... on the concentration of O2forall proteins except the L99F mutant, for which the slow phase was independent of the O2concentration. The rate constants for the fast phase of O2binding to the ... subunit of the dimer [10]. Therefore, it ispossible that the first O2molecule binds quickly toone of the dimers, followed by binding of the secondO2molecule to the other subunit of the dimer,...