Báo cáo khoa học: P NMR studies of energy metabolism in xanthosine5¢-monophosphate overproducing Corynebacterium ammoniagenes pot

... cellular energy metabolism in XMP -overproducing C. ammoniagenes, monitor phosphate-containing metabolites by using 31 P NMR spectroscopy, and discuss cellular energetics in nucleotide production. Experimental ... substrates for XMP production. During phase I, ATPb and ATPc plus ADPb signals were maintained at very low intensity levels. During phase II (hypoxanthine producing p...

Ngày tải lên: 23/03/2014, 17:22

... + GAP « FBP – TKL 7 F 6P + GAP « E 4P + X 5P – Aldo2 8 E 4P + DHAP « SBP – TKL2 10 GAP + S 7P « X 5P + R 5P – R5Piso 11 R 5P « Ru 5P – X5Pepi 12 X 5P « Ru 5P – PGI 14 F 6P « G 6P – PGM 15 G 6P « G 1P – StPase ... Starch + P i fi G 1P – Export processes TPT 18 PGA + P icyt fi P i + PGA cyt – TPT 18 GAP + P icyt fi P i + GAP cyt – TPT 18 DHAP + P icyt fi P i + DHAP cyt...

Ngày tải lên: 20/02/2014, 23:20

... methylglyoxal despite the absence of a complete glyoxalase pathway. Products of trypanosomatid metabolism of methylglyoxal In all studies to date, the principal product of thiol- dependent metabolism of methylglyoxal ... means of three independent experi- ments. b All data represent the mean ± SD of six determinations from two independent experiments. c Cell lines were g...

Ngày tải lên: 23/03/2014, 06:20

... polyproline type II helix [47]. Thus, the MBP segment that we have studied may be critical in the protein’s interaction with the myelin membrane, potentially in proper positioning of this putative ... Catz I & Wucherpfennig KW (1997) Tol- erance induction to myelin basic protein by intravenous synthetic peptides containing epitope P8 5VVHFFKNIVTP96 in chronic progressive multiple...

Ngày tải lên: 19/02/2014, 07:20

... 3-polyols. The vast family of polyenes antibiotics [1,2] includes amphiphilic compounds mostly produced by Streptomyces species with potent antifungal properties. Polyene macro- lides are of ... make of polyene macrolides a source of lead structures for the engineering of future molecules with improved medicinal purposes. In parti- cular, the gene clusters involved in the biosyn...

Ngày tải lên: 21/02/2014, 03:20

... unique coordinates for all protons of interest except the Tyr32(B10) hydroxyl proton, as its position was determined from the 1 H NMR spectral parameters. Results The 1 H NMR spectra of Pe WT metHbCN in 1 H 2 Oand 2 H 2 O ... (1992) Complete sequence-specific 1 hnmr resonance assignment of hyperfine-shifted residues in the active site of a paramagnetic protein: application to Ap...

Ngày tải lên: 23/03/2014, 17:22

... tail containing six putative metal-binding domains. Homologues of HAH1 and Keywords copper(I); metal homeostasis; metallochaperone; protein–protein interaction Correspondence I. Bertini, Magnetic ... observed between apo– MNK5 and copper(I)–MNK5 indicate that perturba- tions due to copper(I) binding affect mainly the Cys- containing loop (loop 1). Indeed, the comparison of the two structu...

Ngày tải lên: 30/03/2014, 15:20

... with DPP IV, but its associ- ation is dependent of insulin with maximal association coincident with maximal tyrosine phosphorylation of DPP IV in vivo (Fig. 2). Insulin-dependent DPP IV phosphorylation ... of [c- 32 P] ATP, and immuno- precipitation with anti-DPP IV IgG, a phosphorylated protein of appropriate apparent molecular mass (110 kDa) was observed (Fig. 3A). DPP IV 32 P...

Ngày tải lên: 19/02/2014, 07:20

... annotation as displayed in the Chat & Event Panel. Figure 9 shows the corresponding se- ries of progressive moments in time portraying en- try into the Bank subzone as represented in RAT. In the ... and space. While the design of the RAT tool will support annotation of any event of interest with only slight modifications, for our purposes, we were interested in anno...

Ngày tải lên: 20/02/2014, 04:20

... unlabeled corpus by querying the in- 0 :p MN:pN N:pM N :p N:pN MN :p MN:pMN:pMN MN:pMN Figure 1: Lattice of pairs of potential attach- ment s ite (NP) and attachment phrase (PP). M: premodifying adjective ... de- pendency parser. The corpus is parsed and all extracted dependencies are stored for later use. Dependencies in ambiguous PP attachments (those corresponding to [VP NP PP]...

Ngày tải lên: 20/02/2014, 12:20