Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc

... antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes Laure Be ´ ven 1 , Sabine Castano 2 , Jean Dufourcq 2 ,A ˚ ke ... Hence, the anti- bacterial action of these peptides comprises four main steps: step 1, interfacial partitioning and exofacial accumul...

Ngày tải lên: 23/03/2014, 17:21

... in the context of language generation, while we search for the best alignment on the condition that both trees are given. To facilitate the presentation of the tree align- ment model, the ... Precision 93.5% 97.2% Table 1: Precision of Mined Parallel Documents The document-level mining precision solely depends on the candidate document pair verifica- tion mo...

Ngày tải lên: 08/03/2014, 02:21

... sequence of words. The distance of two surface patterns is defined as the fraction of the LD value to the length of the longer sequence. For estimating the number of clusters k, we ap- ply the stability-based ... good precision using linguistic patterns, whereas surface patterns from Web frequency information contribute greatly to the coverage of relation extracti...

Ngày tải lên: 23/03/2014, 16:21

... a comparison of the thermodynamic and chemical stability of the NCD form of BS-RNase with that of the monomeric derivative, together with an investigation of the thermal dissociation mechanism revealing ... stabilities, indicating that the substitution of four residues in the hinge region does not significantly perturb the global stability of the monomeric form o...

Ngày tải lên: 22/03/2014, 17:20

... below). Therefore, the mutation of the glutamine residue at position 116 results in the loss of zinc from the corresponding site of the enzyme under MS conditions. Q116H, like the wild-type, con- tains ... that the active form of the enzyme contains only one zinc ion, located in the DHH site. This is in contrast to all known B1 and B3 MBLs, with the possible exce...

Ngày tải lên: 14/03/2014, 23:20

... levels of fractions from reverse-phase HPLC separation of the tryp- tic peptides from autophosphorylated AfRio1. (C–E) Edman degradation of pep- tides obtained from the radioactive fraction of HPLC ... which marks the start of aC. The entire flexible loop packs between the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A). The interactions betwe...

Ngày tải lên: 30/03/2014, 20:20

... effect on the functionality of the vacuole as a result of the accumulation of partially hydrolysed peptides, leading to the accumulation of uncleaved globin [39]. The possible effect, either direct or ... subtracted from the inset data. Plots of percent- age control activity versus concentration of inhibitor were used to determine the concentrations that inhibi...

Ngày tải lên: 14/02/2014, 14:20

... two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12]. The concentration of GTP in the cytoplasm of an exponentially ... example, the GTPase activity of TorD is dependent upon the presence of magnesium ions and this is clearly in line with the requirements of the classical G...

Ngày tải lên: 16/02/2014, 09:20

... stabilize the oxyani- on that develops during cleavage of the peptide bond. In contrast, the c-amide hydrogen of the Asn164 resi- due of E78H ⁄ D164N may participate in stabilization of the transition ... by the addition of the enzyme (up to 50 lL). After incubation for 10 min, the reaction was stopped by the addition of 300 lL1m Tris ⁄ HCl, pH 9.5; the mixtu...

Ngày tải lên: 19/02/2014, 07:20

... analysis, and the determination of released ammonia. The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step. The product ... characterization of the purified enzyme The c oncentrated enzyme solution (fraction 6) was yellow i n color. The enzyme s olution showed one main absorption peak...

Ngày tải lên: 19/02/2014, 16:20