Báo cáo khoa học: An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase docx

... An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase Hisashi Hemmi, Motoyoshi Noike, Toru Nakayama and Tokuzo Nishino Department of ... Conversion of product specificity of archae- bacterial geranylgeranyl- diphosphate synthase. Identification of Fig. 6. Proposed mechanism of product determi...

Ngày tải lên: 23/03/2014, 17:21

... made man- ifest in several ways. Fine-grained syntactic anal- ysis can be pursued by imposing on the deriva- tion tree further combinatoriM constraints, for instance, selective adjoining constraints ... 3.3 Adding Semantics Finally, the formation of synchronous TAGs has been proposed to allow use of TAGs in semantic interpretation, natural language generation, and machine tra...

Ngày tải lên: 20/02/2014, 21:20

... synchronized and incubated for 24 h in Ham’s F10 medium containing serum and vitamin D 2 or its metabolites, and [ 3 H]-thymidine. (B) HaCaT keratinocytes were transfected with a construct containing ... region in meta- bolite 2 was cleaner and similar to that of metabolite 1, indicating the presence of 20-OH and no other hydroxyl group on the side chain (Fig. 3D). The A-ring and doub...

Ngày tải lên: 23/03/2014, 10:21

... that this kind of training can be effectively applied on a large treebank. 1 Introduction The LR parsing strategy was originally devised for programming languages (Sippu and Soisalon- Soininen, 1990), ... An alternative method of training probabilistic LR parsers Mark-Jan Nederhof Faculty of Arts University of Groningen P.O. Box 716 NL-9700 AS Groningen The Netherlands markjan@let...

Ngày tải lên: 31/03/2014, 03:20

... zebrafish RPE65a and RPE65c. Fig. S2. Immunostaining of zebrafish retinal section using antibodies for GS and RPE65c. Fig. S3. Hypothesized molecular mechanisms of the in vitro assay system and the intra-retinal ... con- taining atRE (A), liposomes containing atRE in the presence of 1m M bipyridine (B) and liposomes containing atRE, in the presence of 1 m M bipyridine and 6 mM FeS...

Ngày tải lên: 14/02/2014, 14:20

... grouping of proteins sharing type- unique characteristics. Thus, a key cluster of proteins has evolved to play various roles in a variety of organ- isms [14]. It is thus important to understand ... the heme plane. This novel location and arrangement seems to produce the swing mechanism. However, this swing mechanism may be needed in type C and D DyP -type peroxidases. Never...

Ngày tải lên: 06/03/2014, 00:20

... western blot analysis and an in vi- tro enzymatic assay. A faint, yet single band was observed in both the total brain homogenates and in the membrane fraction of the brain (Fig. 7B). The band 0 40 80 120 160 200 A B C D E 13-cis ... and sensitive method for the quantitation of microgram quantities of protein uti- lizing the principle of protein-dye binding. Anal Biochem 72, 2...

Ngày tải lên: 29/03/2014, 00:20

... 3 [4–7]. In addition to binding DNA, the HD is a protein interac- tion module that mediates interactions with other DNA-binding proteins and non-DNA-binding tran- Keywords homeodomain; Meis; ... the BglII and HindIII sites, as previously described [18]. Dou- ble-stranded oligonucleotides containing either a consensus Meis2-binding and Pbx1-binding site or the Pbx–Hox-bind- ing site from ......

Ngày tải lên: 16/02/2014, 15:20

... protein kinase 1 (DAPK-1) is a Ca 2+ ⁄ calmodulin-regulated serine ⁄ threonine kinase composed of multiple functional domains, including a kinase domain, a calmodulin-binding domain, eight ankyrin ... encompassing part of the ankyrin-repeat domain, the P-loop motifs, part of the cytoskeletal binding domain of DAPK-1, and a unique C-terminal ‘tail’ extension not present in DAPK-1. Ex...

Ngày tải lên: 18/02/2014, 17:20

... glyoxylate cycle mutants [5]. Determination of maintenance Maintenance is the energy that is u sed for survival of cells without any biomass formation. Determination of main- tenance was based on t he inhibition ... of 2 lmol NADPÆmin )1 Æmg protein )1 . Determination of maintenance In order to calculate the amount of glucose used for maintenance, the wild -type strain A2...

Ngày tải lên: 19/02/2014, 16:20