Báo cáo khoa học: An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase docx
Báo cáo khoa học: An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase docx
... An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase Hisashi Hemmi, Motoyoshi Noike, Toru Nakayama and Tokuzo NishinoDepartment of ... Conversion of product specificity of archae-bacterial geranylgeranyl- diphosphate synthase. Identification of Fig. 6. Proposed mechanism of product determination in type III GGPSs.Ó FEBS 2003 Another ... strict product specificities, and the mecha-nisms involved in product determination have so far beeninvestigated using FPSs and geranylgeranyl diphosphate synthases (GGPSs) that yield C15and...
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Tài liệu Báo cáo khoa học: "An Alternative Conception of Tree-Adjoining Derivation*" ppt
... made man- ifest in several ways. Fine-grained syntactic anal- ysis can be pursued by imposing on the deriva- tion tree further combinatoriM constraints, for instance, selective adjoining constraints ... 3.3 Adding Semantics Finally, the formation of synchronous TAGs has been proposed to allow use of TAGs in semantic interpretation, natural language generation, and machine translation. In previous ... Computer and Information Science, University of Pennsylvania, April. Anthony Kroch. 1989. Asymmetries in long dis- tance extraction in a tag grammar. In M. Baltin and A. Kroch, editors, Alternative...
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Báo cáo khoa học: An alternative pathway of vitamin D2metabolism Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2and 17,20-dihydroxyvitamin D2 pot
... synchronized and incubated for 24 h in Ham’s F10medium containing serum and vitamin D2or its metabolites, and[3H]-thymidine. (B) HaCaT keratinocytes were transfected with aconstruct containing ... region in meta-bolite 2 was cleaner and similar to that of metabolite1, indicating the presence of 20-OH and no otherhydroxyl group on the side chain (Fig. 3D). TheA-ring and double bond linker ... position and coup-ling patterns in vitamin D2and metabolite 2, indica-ting that the B-ring stays intact. Therefore, the secondhydroxylation must occur in the C-ring. The 14-CH in this metabolite...
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Báo cáo khoa học: "An alternative method of training probabilistic LR parsers" pptx
... that thiskind of training can be effectively applied on a largetreebank.1 IntroductionThe LR parsing strategy was originally devisedfor programming languages (Sippu and Soisalon-Soininen, 1990), ... An alternative method of training probabilistic LR parsersMark-Jan NederhofFaculty of ArtsUniversity of GroningenP.O. Box 716NL-9700 AS GroningenThe Netherlandsmarkjan@let.rug.nlGiorgio ... Rules and transitions al-most stand in a one-to-one relation. The only note-worthy difference is between transitions of type (6)and rules of type (12). The right-hand sides of thoserules can...
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Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc
... zebrafishRPE65a and RPE65c.Fig. S2. Immunostaining of zebrafish retinal sectionusing antibodies for GS and RPE65c.Fig. S3. Hypothesized molecular mechanisms of the in vitro assay system and the intra-retinal ... con-taining atRE (A), liposomes containing atRE in the presence of 1mM bipyridine (B) and liposomes containing atRE, in the presence of 1 mM bipyridine and 6 mM FeSO4(C). The generated retinoidswere ... Immunostaining of dissociated retinalcells using antibodies for RPE65c and GS. (C1–10) The representative images of RPE65c staining (C1, C6), GS staining (C2, C7), mergedRPE65c and GS staining (C3,...
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Báo cáo khoa học: The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue potx
... grouping of proteins sharing type- unique characteristics. Thus, a key cluster of proteinshas evolved to play various roles in a variety of organ-isms [14]. It is thus important to understand ... the heme plane. This novel location andarrangement seems to produce the swing mechanism. However, this swing mechanism may be needed in type C and D DyP -type peroxidases. Nevertheless, webelieve ... theresting state, two deduced intermediates, and compound I are shown. The two black bars enclosing an iron atom indicate the porphyrin ring.Broken lines and arrows indicate hydrogen bonds and...
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Báo cáo khoa học: An enzymatic mechanism for generating the precursor of endogenous 13-cis retinoic acid in the brain docx
... western blot analysis and an in vi-tro enzymatic assay. A faint, yet single band wasobserved in both the total brain homogenates and in themembrane fraction of the brain (Fig. 7B). The band04080120160200ABCDE13-cis ... and sensitive method forthe quantitation of microgram quantities of protein uti-lizing the principle of protein-dye binding. AnalBiochem 72, 248–254.66 Ma J, Zhang J, Othersen KL, Moiseyev ... (2008)Quantitative profiling of endogenous retinoic acid in vivo and in vitro by tandem mass spectrometry. AnalChem 80, 1702–1708.4 Giguere V (1994) Retinoic acid receptors and cellularretinoid binding...
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Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt
... 3 [4–7]. In addition to binding DNA, the HD is a protein interac-tion module that mediates interactions with otherDNA-binding proteins and non-DNA-binding tran-Keywordshomeodomain; Meis; ... theBglII and HindIII sites, as previously described [18]. Dou-ble-stranded oligonucleotides containing either a consensusMeis2-binding and Pbx1-binding site or the Pbx–Hox-bind-ing site from ... the interaction of Pbx1 with the Hth domain of Meis2. Target-ing of the Hth domain to DNA suggests that it is not a portable trans-acting repression domain. However, the Hth domain can inhibit...
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Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... protein kinase 1 (DAPK-1) is aCa2+⁄ calmodulin-regulated serine ⁄ threonine kinasecomposed of multiple functional domains, including akinase domain, a calmodulin-binding domain, eightankyrin ... encompassing part of the ankyrin-repeat domain, the P-loopmotifs, part of the cytoskeletal binding domain of DAPK-1, and a uniqueC-terminal ‘tail’ extension not present in DAPK-1. Expression of s-DAPK-1 ... 5¢-TAAGGCCACAGGGTCCAGTA-3¢.Immunostaining and membrane-blebbing assayA375 cells were analyzed by immunostaining and mem-brane blebbing. Twenty-four hours post-transfection, cellswere fixed with 4% paraformaldehyde in NaCl...
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Tài liệu Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans doc
... glyoxylatecycle mutants [5]. Determination of maintenanceMaintenance is the energy that is u sed for survival of cellswithout any biomass formation. Determination of main-tenance was based on t he inhibition ... of 2 lmolNADPÆmin)1Æmg protein)1. Determination of maintenance In order to calculate the amount of glucose used formaintenance, the wild -type strain A26 was used. Four100-mL aliquots of ... addition of acetate to the media containing glucoseand propionate, the growth rate of both strains increasedand the effect of propionate became less apparent. Finally, in media containing acetate and...
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