Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx

... identified in yeast) PEX17. Several other peroxins are involved in the subsequent steps of the import. The import of matrix proteins seems to involve a cascade of interactions between the cargo-loaded ... lower (not shown). In order to determine the in uence of the reduction of the expression of TbPEX14 on the import of glycosomal matrix prote...

Ngày tải lên: 23/03/2014, 17:21

... SH3 domain in the same protein, and that this intramolecu- lar interaction suppresses the lipid-binding activity of the PX domain in the resting state; phosphorylation of p47 phox releases the binding, ... domains play important roles in the function of proteins contain- ing this domain [40]. In particular, p47 phox , which con- tains the PX domain and two SH...

Ngày tải lên: 16/03/2014, 04:20

... the interpretation of IR spectra of mineral deposits. Distinct mineralization steps were observed during the GPI-bALP- induced hydrolysis of b-GP. At incubation times in the range 1.5–3 h, two bands ... France. Results Evidence of different steps in the GPI-bALP mediated mineralization: effects of Ca 2+ concentration To assess the role of Ca 2+ in initiating th...

Ngày tải lên: 23/03/2014, 17:21

... research is that understanding what causes the loss of appropriate metal binding might be crucial for the understanding of the role of amyloidogenic proteins in a number of neurodegenerative disorders. Experimental ... proteins they probably form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another hist...

Ngày tải lên: 19/02/2014, 06:20

... nonpeptide caspase-3 inhibitors. The results of dilution and dialy- sis experiments indicated that our compounds are irre- versible and slow-binding inhibitors. Research on the inhibitory mechanism of isoquinoline-1,3,4-trione ... XhoI and NheI ⁄ XhoI, caspase 2, 3, 7 and 9 cDNA with the nucleotide fragments encoding the His6 tag at the C-terminus of the recombinant...

Ngày tải lên: 07/03/2014, 11:20

... from the candidate models that best describe the input–output experimental data. T he individual components of the selected model provide insights into the importance of their roles in the overall ... dominance of these individual elements, which are captured by parameters s uch as the binding constants and the extent of autoregulation. The regulatory proteins ma...

Ngày tải lên: 07/03/2014, 16:20

... may AB Fig. 5. The role of the C-terminal, HBXIP interacting, domain of hSuv3p in protein stability. (A) The protein stability of the wildtype hSuv3TAP and the hSuv3TAP variant lacking the C-terminal 136 ... absence of HBXIP binding domain resulted in  50% decline in the truncated protein levels within 4 h following inhibition of protein synthesis by cyclohe...

Ngày tải lên: 23/03/2014, 15:21

... region of the bilayer. On the other hand, the presence of cholesterol reduced the tilt of the MSI-594 helix to within 5° and that of the MSI-78 peptide to < 5°. This observation on the reduction ... cecropin and magainin), b-sheet or b-hairpin, stabilized by disulfide bonds (e.g. defensins, tachyplesins, and protegrins), and extended (e.g. indo- licidin and P...

Ngày tải lên: 29/03/2014, 22:21

... chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... of the pro- tein in the N state and the evolution of the overall structure of the protein in the MG state. This degree of resolution is unpreceden...

Ngày tải lên: 16/02/2014, 09:20

... distributing origins, including hyperthermophiles, thermophiles and mesophiles. Background: there is no single dominating factor for the thermostability of proteins Many crystal structures of hyperthermophilic ... cooperative interaction within the hydro- phobic protein core plays an integral role in increasing the thermalstability. Another potential stabilizing fac- tor is...

Ngày tải lên: 07/03/2014, 05:20