Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... residues at the IN C-terminal domain in the folding and dimerization of the enzyme as well as in the concerted DNA integration of viral DNA ends.Keywords: concerted DNA integration; integrase; ... (IN) is the retroviral enzyme responsible for the integration of the DNA copy of the retroviral genome into the host cell DNA. The C-terminal domain of IN is involved in DNA binding and enzyme ... the terminal part of the C-terminal domain of ALSV integrase in the general folding of the enzyme and, hence, in its activity in the concerted DNA integration assay.According to the structure proposed...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... to the formation of cavities, one in the hydrophobiccore (M88V) and the other in the protein–RNA interaction(T45S), decrease the stability of the capsid. Our findingsilluminate the role of ... different from those of other simple icosahedralviruses [13]. The main chain of the protein subunit foldsinto a five stranded antiparallel b-sheet (strands bC–bG)facing the interior of the phage particle, ... Asymmetric interactions in the adenosine-binding pockets of the MS2 coat protein dimer.BMC Mol Biol. 2,6.48. Lima, L.M.T., Foguel, D. & Silva, J.L. (2000) DNA tightens the dimeric DNA- binding domain...
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Báo cáo khoa học: Glycosphingolipids in Plasmodium falciparum Presence of an active glucosylceramide synthase pot
... treatment of parasitecultures with PPMP resulted in a potent inhibition of the intraerythrocytic development of P. falciparum [27–30]. In order to determine the effect of the inhibitor in GSLssynthesis, ... confirm that the de novobiosynthetic pathway of ceramides is active in this parasite.Nevertheless, the last step of the ceramide biosynthesis,involving the dehydrogenation of N-acylsphinganine toN-acylsphingenine ... with the mobility of an authentic standard of C18-sphinganine, the other one (RF0.38) migrating slightlyabove, would correspond to C20-sphinganine. A similarresult was obtained when the...
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Báo cáo khoa học: Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes potx
... that the rate of platelet nuc-leotide hydrolysis is lower in platelets than in endothelium. However, considering the function and the mobility of platelets, we can understand theiractive role in ... this process and the importance of such hydrolysis. In line with this, literature data indi-cate that adenine nucleotides and adenosine areimportant modulators of atherosclerosis [18], and that ... consisting of the in vitro macroscopic visualization of aggregates at intervals of 15–50 s by the addition of ADP to PRP.Determination of lipid peroxidationLipid peroxidation was estimated by the...
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Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx
... rate-limiting in providing precur-sors for synthesis of the cell wall [13,15,16]. However,it has been impossible to obtain direct confirmation of the hypothesis on the basis of the size of the UDPpool ... 40-kDa band within the TOY2 and TOY3strains (Fig. 2A). In vitro activities of UGD and UXSWe next examined the activity of the hybrid enzymesfrom cytoplasmic fractions of the recombinant TOY1cells. ... comparableto that in the W303a strain. To gain a better under-standing of how the amount of UDP-sugars wasregulated in the cells, we analyzed several properties of the recombinant AtUGD1. The substrate...
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Báo cáo khoa học: "Parsing with an Extended Domain of Locality" pot
... the morphologically analysed words in the input sentence. For example, the tree shown on the right is the result of anchoring the tree shown on the left with the word loves. An- choring instantiates ... sequence of parser actions that involve finding a NP constituent on the right (corresponding to the object), possibly perform- ing adjunctions at the VP node, and then finding another NP constituent ... trees. In predica- tive auxiliary trees the anchor is also the head of the tree and becomes the head of the tree resulting from the adjunction. In modifier auxiliary trees, the anchor is not the...
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Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx
... both in the presence and in the absence of calcium. On the other hand the TnI inhibitory and C-terminal domains were completelydigested by trypsin in the absence of calcium while the addition of ... the N domain of TnC binds in a calcium-dependent manner to the C domain of TnI, releasing the inhibitory effect of the latter on the actomyosin interaction. While the crys-tal structure of the ... involved in the binding to N domain of TnC [11–16]. Serialdeletion studies of the C terminus of TnI showedthat residues 166–182 are involved in binding to the thin filament [16]. Altogether, these...
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Báo cáo khoa học: Hyperefficient PrPSc amplification of mouse-adapted BSE and scrapie strain by protein misfolding cyclic amplification technique pptx
... also result in an increased risk of contamination. In thisstudy, we sought to further promote the rate of PrPScamplification in the protein misfolding cyclic amplification technique using mouse ... detection, and alsominimize the risk of contamination. The approximately 10-fold difference in the sensitiv-ity between mBSE and Chandler may be caused by dif-ferences between the minimum size of ... provide the opportunityfor treatment of TSEs. The key molecular event in the progression of TSEsis the continuous conformational conversion of the normal cellular form of prion protein (PrPC)...
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Báo cáo khoa học: "A Competition-Ba sed Explanation of Syntactic Attachment Preferences and Garden Path Phenomena" pot
... attachments between the current phrase and the right edge of the existing parse tree. Limiting the attachment of the current phrase to the right edge of the parse tree rules out all of the problematic ... tests the equality conditions that were developed to encode the following subset of GB constraints: the Theta Criterion, the Case Filter, categorial selection, and the binding of traces. The ... representation of the grammar of a cur- rent linguistic theory. In Government-Binding theory (GB) (Chomsky 81, 86; Rizzi 90), the validity of syn- tactic structures is achieved by locally satisfying the...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... blotting of modified proteinsWe identified the major proteins in the mitochondrialmatrix by performing SDS/PAGE separation and staining the gel with Coomassie blue or Western blotting. Analysis of ... decrease in the amount of this protein in the matrix. We have not detected any significant modifica-tion in the amount of this protein in the matrix. We canconclude that the decrease in this enzymatic ... Mitochondria are in fact a majorintracellular source of ROS during oxidative phosphoryla-tion and the increased production of ROS is implicated in the aging process [12–16]. Mitochondria are also the majortargets...
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Từ khóa: Nghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015