Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... residues at the IN C-terminal domain in the folding and dimerization of the enzyme as well as in the concerted DNA integration of viral DNA ends. Keywords: concerted DNA integration; integrase; ... (IN) is the retroviral enzyme responsible for the integration of the DNA copy of the retroviral genome into the host cell DNA. The C-term...
Ngày tải lên: 23/03/2014, 15:21
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... to the formation of cavities, one in the hydrophobic core (M88V) and the other in the protein–RNA interaction (T45S), decrease the stability of the capsid. Our findings illuminate the role of ... different from those of other simple icosahedral viruses [13]. The main chain of the protein subunit folds into a five stranded antiparallel b-sheet (strands bC–bG) fa...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Glycosphingolipids in Plasmodium falciparum Presence of an active glucosylceramide synthase pot
... treatment of parasite cultures with PPMP resulted in a potent inhibition of the intraerythrocytic development of P. falciparum [27–30]. In order to determine the effect of the inhibitor in GSLs synthesis, ... confirm that the de novo biosynthetic pathway of ceramides is active in this parasite. Nevertheless, the last step of the ceramide biosynthesis, involving...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes potx
... that the rate of platelet nuc- leotide hydrolysis is lower in platelets than in endothelium. However, considering the function and the mobility of platelets, we can understand their active role in ... this process and the importance of such hydrolysis. In line with this, literature data indi- cate that adenine nucleotides and adenosine are important modulators o...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx
... rate-limiting in providing precur- sors for synthesis of the cell wall [13,15,16]. However, it has been impossible to obtain direct confirmation of the hypothesis on the basis of the size of the UDP pool ... 40-kDa band within the TOY2 and TOY3 strains (Fig. 2A). In vitro activities of UGD and UXS We next examined the activity of the hybrid enzymes from cy...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: "Parsing with an Extended Domain of Locality" pot
... the morphologically analysed words in the input sentence. For example, the tree shown on the right is the result of anchoring the tree shown on the left with the word loves. An- choring instantiates ... sequence of parser actions that involve finding a NP constituent on the right (corresponding to the object), possibly perform- ing adjunctions at the VP node...
Ngày tải lên: 08/03/2014, 21:20
Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx
... both in the presence and in the absence of calcium. On the other hand the TnI inhibitory and C-terminal domains were completely digested by trypsin in the absence of calcium while the addition of ... the N domain of TnC binds in a calcium-dependent manner to the C domain of TnI, releasing the inhibitory effect of the latter on the actomyosin...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Hyperefficient PrPSc amplification of mouse-adapted BSE and scrapie strain by protein misfolding cyclic amplification technique pptx
... also result in an increased risk of contamination. In this study, we sought to further promote the rate of PrP Sc amplification in the protein misfolding cyclic amplification technique using mouse ... detection, and also minimize the risk of contamination. The approximately 10-fold difference in the sensitiv- ity between mBSE and Chandler may be caused by dif- ferenc...
Ngày tải lên: 23/03/2014, 04:21
Báo cáo khoa học: "A Competition-Ba sed Explanation of Syntactic Attachment Preferences and Garden Path Phenomena" pot
... attachments between the current phrase and the right edge of the existing parse tree. Limiting the attachment of the current phrase to the right edge of the parse tree rules out all of the problematic ... tests the equality conditions that were developed to encode the following subset of GB constraints: the Theta Criterion, the Case Filter, categorial...
Ngày tải lên: 31/03/2014, 06:20
Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... blotting of modified proteins We identified the major proteins in the mitochondrial matrix by performing SDS/PAGE separation and staining the gel with Coomassie blue or Western blotting. Analysis of ... decrease in the amount of this protein in the matrix. We have not detected any significant modifica- tion in the amount of this protein in the matrix. We can conc...
Ngày tải lên: 20/02/2014, 11:20