Báo cáo khoa học: K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase pdf

... findings suggest that human cells contain histone H3, biotinyl- ated at K4, K9 and K18. Discussion This study provides evidence (a) that K4, K9 and K18 in histone H3 are good targets for biotinylation ... biotinylation by human biotinidase; (b) that K14 and K23 are relatively poor targets for biotinylation; (c) that human cells contain hist...

Ngày tải lên: 23/03/2014, 15:20

... alanine at position 17 in H1.2 was replaced by valine, and, in Raji B lymphoblastoid cells, lysine at position 173 in H1.4 was replaced by arginine. We confirmed these findings by DNA sequencing ... variants is unclear, but increas- ing evidence indicates that minor sequence variations in linker histones may change their binding characteristics, in uence chromatin remodeling, a...

Ngày tải lên: 30/03/2014, 20:20

... proteins. Thus, the MEN1 ⁄ LEDGF-interacting domain linked to DNA-binding domains (AT-hook and MT domain) becomes disconnected from the PHD domains, the FYRN domain, the transactivating domain, ... DRB-sensitivity inducing factor; GSK, glycogen synthase kinase; H 3K4, histone H3 lysine 4; HMT, histone methyltransferase; MLL, mixed lineage leukemia; NELF, negative elongation factor; P...

Ngày tải lên: 16/02/2014, 14:20

... occur- ring in many different combinations are the P-loop nucleotide triphosphate hydrolase domain, the epider- mal growth factor (EGF) domain, the SH3 domain, the P-kinase domain and the domains involved ... percentages of domains that form autonomous, single-domain proteins are higher for domains that undergo deletions at the termini, and lower for domains that undergo deletio...

Ngày tải lên: 19/02/2014, 07:20

... (BACKCHANNEL and OTHER are merged) using hand-labeled data of a single meeting as a test set and the remaining data as training material; for this experiment, we used the same training set as (Hillard ... first performed several empirical analyses in or- der to determine to what extent contextual informa- tion helps in discriminating between agreement and disagreement. By integ...

Ngày tải lên: 20/02/2014, 16:20

... of the words in the sentence Gilbert walks are as shown in (27), before binding. Binding of string positional variables yields the cat- egories in (28). The combinatory rule of forward application ... outlined above, and file latter must be greatly constrained for English.) One very general constraint, excluding all combinations with or into NP, is included in the progr...

Ngày tải lên: 20/02/2014, 21:20

... droplets in adipose cells, leading to an increased fat mass and ultimately result- ing in obesity. Adipocyte hyperplasia (increase in cell number) and hypertrophy (increase in cell size) are thought ... kinase-associated protein (Skp)1–Cullin- F-box protein (SCF) ubiquitin ligase (E3) complex targets cyclin-dependent kinase inhibitors for degrada- tion by the 26S proteasom...

Ngày tải lên: 06/03/2014, 01:20

... some 7 A ˚ to fill the active site and pack against the FAD cofactor. In the loop between b-strand B6 in the substrate-binding domain and strand E2 in the hinge domain, two residues appear to act, ... determined. Variant 60 °C70°C75°C Inactivation constant k in, 1 (min )1 ) Inactivation constant k in, 2 (min )1 ) Half-life s 1 ⁄ 2 (min) Inactivation constant k in (min )1 ) Half-l...

Ngày tải lên: 07/03/2014, 03:20

... cytokines in vivo and in vitro [22], by converting inactive precursors of cytokines such as pro-IL-1b and pro-IL-18 into their active forms [18,23], or by enhancing the expression of cytokine genes ... extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) but not p38 MAPK. Unexpectedly, an inhibitor for JNK but not for MAPK ⁄ ERK kinase inhibited the...

Ngày tải lên: 07/03/2014, 09:20

... assays Equilibrium and kinetic parameters for SSA binding to different TCR b chains were determined in a resonance mirror using an IAsys instrument biosensor. SSA affinity for the immobilized ... large domains, which has been shown to b e the TCR binding site in other SAgs. Point mutation of Cys26 to Ser prevented dimer formation and allowed TCR interaction studies. Initial binding...

Ngày tải lên: 07/03/2014, 16:20