Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx

Báo cáo khoa học: Characterization of a functionally expressed dipeptidyl aminopeptidase III from Drosophila melanogaster doc

... induced S2cells in NaCl/Picontainingthe aminopeptidase inhibitor bestatine (100 lM), 6.25 nmol of proctolin and 1.75 nmol Arg-Tyr (and Leu-Pro-Thr,not shown) were recovered into the incubation ... from the brain of guinea-pig [5] and rat [6]. Angiotensins and enkephalins constitutethe preferred substrates of the rat brain cytosolic DPP III[7]. The routes of degradation of the insect myotropicneuropeptide ... membranesamples and in partially puriﬁed fractions with proctolin(40 lM, 6 nmol) as substrate. The incubation was carriedout in presence of bestatin (100 lM) in a ﬁnal volume of 150 lLfor15minat25°C...

Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) ppt

... up in the gel(Fig. 1B, lane 6). A subsequent incubation of the gel in DPI resulted in inhibition of the lower band only(Fig. 1B, lane 8). On the basis of the size of multimers of ferritin used ... and isolation of kinetoplastsP. serpens strain 1G, originally isolated from its insect vec-tor, was cultured in brain heart infusion medium with10 lgÆmL)1hemin at 26 °C. The kinetoplast–mitochondrialvesicles ... oligomer of the small form. The different in uences of DPI onthe two forms could be caused by its failure to accessthe ﬂavin cofactor in the large form of complex I,whereas the cofactor remains...

Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx

... al. Core-1 b1-3 galactosyltransferases in Drosophila FEBS Journal 272 (2005) 4295–4305 ª 2005 FEBS 4305 Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster Reto ... boxed. Percentages of sequence identity of the D. melanogaster proteins to the human core-1 b3GalT are indicated in theﬁnal column. Core-1 b1-3 galactosyltransferases in Drosophila R. Mu¨ller ... could meanthat salivary O-glycan chains are elongated, thus abro-gating peanut agglutinin binding. Furthermore, thepeanut agglutinin staining in the developing nervoussystem documented by D’Amico...

Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx

... present in several copies in the N-terminal domain of allPEX5s [22,32,38]. In this paper, in vitro experiments showedthat also in T. brucei the N-terminal part of TbPEX14(amino acids 1–146) interacts ... production of a fusion protein bearing anN-terminal extension of 20 residues including a His6-tag. Ata later stage, a point mutation was introduced in the vectorpET28a-TbPEX14-N in order to ... presumably involved in the protein’s anchoring to the membrane. Also in PEX14 of T. brucei a series of hydrophobic residues is found at thecorresponding region of the polypeptide (amino acids...

Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior of the peptidase pdf

... structure–function relationships of proli-dase, hence providing a greater understanding of thecharacteristics of this peptidase, which would be of industrial use in the debittering of fermented foods.Results ... provideinsights into the behaviour of prolidase, thus contrib-uting to applications of prolidase in fermented foodprocessing.Experimental procedures Enzymes and chemicals Enzymes for genetic engineering ... of peptides during fermentationcan ultimately produce proline-containing dipeptides,such as Xaa-Pro and Pro-Xaa. Ishibashi et al. [5]reported two interesting observations regarding pro-line-containing...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... 1343where the relevant increase of m2produces the relevantintensity changes of ML ⁄ TG signals. In principle, suchan increase can be due to both a decrease in c1and anincrease in p1after growth ... speciﬁcally bound tothe induction of apoptosis, in agreement with recentﬁndings [8].Second, the intensity of the ML signal, correspond-ing to TG levels, is increased in irradiated MCF-7cells, ... suspended in NaCl ⁄ Piata concentration between 1–2 · 106mL)1. Next, 0.5 mL of propidium iodide staining solution (10 mg of propidiumiodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... sequences of thecatalytic domain of members of the GH18 familyresulted in the ampliﬁcation of an expected 147 bpsequence. Cloning and sequencing of this fragmentrevealed an ORF showing amino acid ... Crassostrea gigas [5]. Interestingly, thisprotein, named C. gigas chitinase-like protein 1 (Cg-Clp1) was found to be involved in the control of growth and remodelling processes in a manner similarto ... transcripts duringdevelopment and in adult tissuesTo gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution andtemporal pattern of expression during...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... lines indicate intron 6 of 75 bp in fragmentsB and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and3¢ portion of 25 bp in intron 6 in fragments F and I. The number atthe right of ... results in no generation of protein isoforms.The 5¢-UTR of eukaryotic mRNA in uences the initi-ation step of protein synthesis and thereby in partdetermines the translational efﬁciency of the ... genera-tion of protein isoforms differing in amino acidsequence. Human DNASE1 pre-mRNA is transcribedfrom different transcription start sites, exons 1 and 1a,resulting in generation of two kinds of...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding site of PPI1 is localized therein. ... blue-lightactivation of PM H+-ATPase in guard cells of broadbeans involves protein kinase-mediated phosphoryla-tion of Ser and Thr residues in the C-terminus of thepump and 14-3-3 binding [17]. Much ... bythe parallel increase of the number of FC-bindingsites [18–20]. As to auxin, a soluble auxin receptor hasbeen reported to bind and activate the PM H+-ATPase,but the site of binding has not...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... bolded; N-terminalresidues of the catalytic domain are under-lined. The preregion is indicated in red, thepro-region in black, catalytic domain in blueand the C-terminal domains in violet. Theassumed ... peptidase formincluding two C-terminal domains and one C-terminaldomain, respectively. The protein band at  34 kDarefers to the ‘mature’ peptidase containing the catalyticdomain only.To verify ... and 120 min. At each time point, the samplewas incubated on ice for 5 min and remaining activitytoward Suc-AAPF-pNA was determined. One hundredpercent activity (0 min) is sample incubated...

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