Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx

... induced S 2 cells in NaCl/P i containing the aminopeptidase inhibitor bestatine (100 l M ), 6.25 nmol of proctolin and 1.75 nmol Arg-Tyr (and Leu-Pro-Thr, not shown) were recovered into the incubation ... from the brain of guinea- pig [5] and rat [6]. Angiotensins and enkephalins constitute the preferred substrates of the rat brain cytosolic DPP III [7]. The routes of degradation o...

Ngày tải lên: 17/03/2014, 10:20

... up in the gel (Fig. 1B, lane 6). A subsequent incubation of the gel in DPI resulted in inhibition of the lower band only (Fig. 1B, lane 8). On the basis of the size of multimers of ferritin used ... and isolation of kinetoplasts P. serpens strain 1G, originally isolated from its insect vec- tor, was cultured in brain heart infusion medium with 10 lgÆmL )1 hemin at 26 °C. T...

Ngày tải lên: 07/03/2014, 09:20

... al. Core-1 b1-3 galactosyltransferases in Drosophila FEBS Journal 272 (2005) 4295–4305 ª 2005 FEBS 4305 Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster Reto ... boxed. Percentages of sequence identity of the D. melanogaster proteins to the human core-1 b3GalT are indicated in the final column...

Ngày tải lên: 23/03/2014, 15:20

... present in several copies in the N-terminal domain of all PEX5s [22,32,38]. In this paper, in vitro experiments showed that also in T. brucei the N-terminal part of TbPEX14 (amino acids 1–146) interacts ... production of a fusion protein bearing an N-terminal extension of 20 residues including a His 6 -tag. At a later stage, a point mutation was introduced in the vector p...

Ngày tải lên: 23/03/2014, 17:21

... structure–function relationships of proli- dase, hence providing a greater understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods. Results ... provide insights into the behaviour of prolidase, thus contrib- uting to applications of prolidase in fermented food processing. Experimental procedures Enzymes...

Ngày tải lên: 30/03/2014, 04:20

... 1343 where the relevant increase of m 2 produces the relevant intensity changes of ML ⁄ TG signals. In principle, such an increase can be due to both a decrease in c 1 and an increase in p 1 after growth ... specifically bound to the induction of apoptosis, in agreement with recent findings [8]. Second, the intensity of the ML signal, correspond- ing to TG levels, is increased...

Ngày tải lên: 18/02/2014, 13:20

... sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence. Cloning and sequencing of this fragment revealed an ORF showing amino acid ... Crassostrea gigas [5]. Interestingly, this protein, named C. gigas chitinase-like protein 1 (Cg- Clp1) was found to be involved in the control of growth and remodelling proces...

Ngày tải lên: 19/02/2014, 00:20

... lines indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of ... results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part determines the tran...

Ngày tải lên: 19/02/2014, 06:20

... C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is localized therein. ... blue-light activation of PM H + -ATPase in guard cells of broad beans involves protein kinase-mediated phosphoryla- tion of Ser and Thr residues in the C-terminus of the...

Ngày tải lên: 19/02/2014, 07:20

... bolded; N-terminal residues of the catalytic domain are under- lined. The preregion is indicated in red, the pro-region in black, catalytic domain in blue and the C-terminal domains in violet. The assumed ... peptidase form including two C-terminal domains and one C-terminal domain, respectively. The protein band at  34 kDa refers to the ‘mature’ peptidase containing the catalytic...

Ngày tải lên: 19/02/2014, 07:20