Báo cáo khoa học: Catalytic residues Lys197 and Arg199 ofBacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop ppt

... Catalytic residues Lys197 and Arg199 of Bacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop Bjarne Hove-Jensen, ... interaction of hydrophobic amino acids of b-strands located on either side of the flexible catalytic loop. The tightly packed interface prevents the closure of the loo...

Ngày tải lên: 23/03/2014, 15:20

... but the differentiation between these two types of enzyme is not clear [4]. Looking at the sequences of the two enzymes, both show absolute conservation of the histi- dine residues of the CuA and ... determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS. Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains...

Ngày tải lên: 19/02/2014, 07:20

... of south-east Asia, parts of Africa, and Southern and Central America. The Aedes aegypti mosquito, which prefers to feed on humans, is a carrier of the dengue virus and is commonly found on the ... to hybridize with the probe sequences. Figure 5 shows the overlapping expression patterns for two pairs of genomes for the set of 216 probes. The distribution of th...

Ngày tải lên: 23/03/2014, 11:20

... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig. 3A). Amino acid sequence analysis of the ... instead of as a heterodimer. Second, 8K-BLP was predominantly pro- duced by the fat body, a functional equivalent of the liver and adipose tissue of...

Ngày tải lên: 18/02/2014, 13:20

... example, the prin- cipal eigenvector (i.e., the eigenvector correspond- ing to the largest eigenvalue) is the direction in which the sum of the square of the projections of the row vectors of the ... we are aware of that analyzes the eigenvectors to obtain a two dimensional visualize of the network. Nevertheless, the work does not study the spectrum of...

Ngày tải lên: 22/02/2014, 02:20

... some of these potentially protec- tive reactions can be made. The reaction of • NO 2 with the thiols of GSH or cysteine is fast (  3– 5 · 10 7 m )1 Æs )1 ) [29] and the rate of reaction of ONOO ) with ... occur because of the rapid reaction of thiols with many of the damaging species present in biological systems. Furthermore, because many of these potentially p...

Ngày tải lên: 06/03/2014, 09:22

... mutation of three of these basic residues within the N-domain of ClpA (H94, R86 and R100) did not alter substrate degradation. In contrast mutation of two conserved arginine residues (R90 and R131), ... initiated through productive binding to the substrate via two elements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer. In the case...

Ngày tải lên: 07/03/2014, 05:20

... CK domains. It is not known whether the active sites in each of the contiguous flagellar domains are catalytically competent, and, if so, whether they are capable of acting independently. Here ... MgADP, creatine and nitrate are bound to CKs. This transition involves the movement of two flexible loops (residues 60–72 and 323–333 in both Torpedo and rabbit CyCKs) and at th...

Ngày tải lên: 16/03/2014, 06:20

... identify the active site residues involved in the binding of Glc to the super-open state (Fig. 1C) of this two domain [large (L) and small (S)] enzyme, and thus the site of initia- tion of the global ... in the L-domain (residues N204, D205, N231, E256 and E290) and the S-domain (residues T168 and K169) (Fig. 1B). To identify the contact residues invol...

Ngày tải lên: 30/03/2014, 04:20

... ccording to Parry and Clark [37], expression of the ybiK gene responded to the presence of cysteine and to defects in t he cysBgene,andtheybiK knockou t mutation impaired the use of glutathione ... 30 m in and the pellet was removed. To the supernatant, further PEG8K was added to a concen- tration of 35% and stirring continued for 1 h . The solution was then centri...

Ngày tải lên: 30/03/2014, 15:20