Báo cáo khoa học: A1–Protein Function and Ageing pptx

... pro- Abstracts 95 A1-002 Longevity and survival factors implicated in human ageing and longevity E. Gonos Molecular and Cellular Ageing, National Hellenic Research Foundation, Athens, Greece. E-mail: sgonos@eie.gr Ageing and ... 150 and 47 lm, for BChE w.t. 320 and 23 lm for 2-PAM and HI-6, respectively. Introduced mutations lowered oxime binding affinities for both oximes,...

Ngày tải lên: 23/03/2014, 15:20

... Rbx1, and one of a family of F-box proteins, Fbw7 [17]. Glycogen syn- thase kinase-3b phosphorylates serine and ⁄ or threonine residues near the ubiquitination site in SREBP-1 and SREBP-2, and ... their specific ligands are present, HNF-4 and LRH-1 appear to be exceptional, in that they are con- stitutively active in the abundance of their endogenous ligands, acyl-CoA and phospholi...

Ngày tải lên: 18/02/2014, 13:20

... hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands. These ether strands mimic the distance between the nucleo- base and the diphospho-bridge, but on the other hand likely ... permeabilized T-cells and induced both local and global Ca 2+ signalling in intact T-cells [46]. 8-Azido- and 8-NH 2 -cIDPRE performed similarly (Fig. 4) whereas the h...

Ngày tải lên: 20/02/2014, 01:20

... SAF34 and P4 strongly stained the di-glycosylated band, and mAbs 6H4, SAF60, SAF70 and SAF84 showed the highest staining with the overlapping bands of nonglycosylated full-length PrP C and glycosylated ... within the prion protein sequence (Fig. 1 and Table 1). The two bands of higher molecular masses are the di- and mono-glycosylated isoforms and the band with the lowest molecu...

Ngày tải lên: 19/02/2014, 02:20

... conformation between the receptor activated by cleavage and shedding on the one hand, and the receptor activated by the ligand on the other hand. Cleavage and shedding of a receptor already activated by ... unpredicted non-ER locations and functions. J. Cell. Physiol. 193, 154–163. Ó FEBS 2003 Role of cleavage and shedding in TSHR function (Eur. J. Biochem. 270) 3497 Role of cleav...

Ngày tải lên: 21/02/2014, 00:20

... Materials and methods. Fig. 7. Expression of PKB protein and activity in differently treated and transfected cells. (A) Expression of PKB protein and b-actin (loading control) in differently treated and transfected ... V/0.4 cm and 1000 lF [26]. Cells were selected by G418 and the neomycin-resistant cells were obtained after 2–3 weeks and re-cloned by serial dilution. The stable...

Ngày tải lên: 21/02/2014, 00:20

... three antiparallel strands (1, 2 and 7) and strand 6, which is parallel to strand 2. b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... ele- ments: b-strands (b3, 329–335; b4, 339–344; and b5, 367–372) and a distorted a-helix (a3, 348–356) (Fig. 3B,C). The three b-strands of the minidomain are all antiparallel, and f...

Ngày tải lên: 06/03/2014, 11:20

... of the molecular chaperones and foldases [5,8]. Many chaperones and foldases are present in the ER lumen [9,10]. Among them, PDI (EC 5.3.4.1) is a unique and multifunctional enzyme that exhibits ... various substrates and com- pared with those of hPDI. The recombinant cPDI and hPDI shared similar reductase, oxidase and isomerase activities. PDI exhibits both chaperone and antich...

Ngày tải lên: 07/03/2014, 05:20

... T56C, T56S, T58S and T58V was functional. Expression of mutants T56C, T56S and T58S was associated with reduced growth rate. The double mutants T56M ⁄ T58W and T56M ⁄ T58V were also functional but ... functional and nonfunctional eEF2 mutants. Growth rate of yeast cells (GB) expressing wild-type or mutant functional forms of eEF2 from plasmid pCBG1202 under normal growth conditions (A...

Ngày tải lên: 07/03/2014, 05:20

... dimer of MCAD and ETF are shown for clarity. PDB code for all, 2A1T [25]. a- and b-ETF chains and MCAD are shown as magenta, blue and green cartoons, respectively. The cofactors FAD and AMP are ... shown as atom-coloured sticks with green and magenta carbons for MCAD and ETF, respectively. H. S. Toogood et al. ETF and partners – structure, function and dynamics FEBS Journa...

Ngày tải lên: 07/03/2014, 05:20