... for the nuclear import. Results on mt9suggested that phosphorylation of the tyrosine residueis not related to the nuclear import. Then, the role of these two residues PY in nuclear import of ... expressed for 22 h. The cellswere then fused with 3T3 cells and cultured for another 1 h in the presence of cycloheximide. Arrows point out the murine nucleus. Panelsa–f, fluorescent signals of the ... cells for 22 h to label the nucleus, and the cellswere then fused to nontransfected murine 3T3 cells in the presence of a protein synthesis inhibitor, cyclohexi-mide, and further incubated for...