Báo cáo khoa học: The binding of foot-and-mouth disease virus leader proteinase to eIF4GI involves conserved ionic interactions ppt

... A virus (ERAV) or the 2A proteinase of enteroviruses and human rhinoviruses (HRVs) cleave eIF4GI and eIF4GII so that the eIF4E- binding part of the eIF4G proteins is separated from the bulk of ... both the binding to and cleavage of eIF4GI [26]. However, the results here also do not rule out further interactions between the CTE of Lb pro and the regio...

Ngày tải lên: 23/03/2014, 13:20

... illumin- ating the structural determinants of the IMP binding to RNase A shows that the inhibitor binds to the enzyme in a novel mode. The chemical characteristics of the IMP molecule seem to impose this binding mode ... [45]. The close interaction of the riboses of IMP mol II and III (Fig. 1) seems to be the driving force for the binding mode of IM...

Ngày tải lên: 07/03/2014, 21:20

... briefly outlines the mathematical basis of the method. The second part presents two applications of the method to the metabolism of erythrocytes and of the microorganism, Metylobacterium extorquens AM1 ... measure of the probability of favourable mutational events t hat increase the fidelity of an enzyme in the context of the metabolic network. The princi...

Ngày tải lên: 07/03/2014, 15:20

... not shown). We therefore assume that the fluorescence of bis-ANS of reduced virus in the presence of gangliosides would not be due to the nonspecific binding of the probe to 2-mercaptoethanol- treated-NDV. ... addition, the con- formational change undergone by the HN protein after binding to gangliosides can be completed in the presence of the correct targe...

Ngày tải lên: 30/03/2014, 13:20

... absorbance, others showed a decreased or diminished antigen-dependency. To evalu- ate the OS-fitness of the clone, the ratio of the specific absorbance in the presence of 10 lgÆmL )1 HEL to that in the ... made to clarify the effect of the interface mutations on the antigen- binding affinity. To investigate systematically this unsought relationship between th...

Ngày tải lên: 07/03/2014, 12:20

... presently of the nature of the FtsY–membrane interaction in Archaea. Moreover, the steps leading to deliveryofSRPtothearchaealmembranehaveyettobe described. Towards an understanding of the membrane ... effect on the ability of either FtsY or the FtsY NG domain to bind to the membrane, with floatation of the proteins to the upper gradient fractions occurring...

Ngày tải lên: 16/03/2014, 16:20

... glycine–HCl, pH 2.2, at the end of each binding cycle. To measure the binding properties of Erbicin and ERB- hRNase to the extracellular domain of ErbB2, ECD was immobilized onto the surface of sensor chip ... difficult to obtain simultaneous binding of antibody to two receptor molecules, and the binding is mainly monovalent; this is reflected in the low af...

Ngày tải lên: 23/03/2014, 06:20

... from the active site. In the crystal structure of barley a-amylase 1, oligosaccharide is thus bound to the ‘sugar tongs’ site. This site on the non-catalytic domain C in the C-terminal part of the ... was hypothesized that the ‘sugar tongs’ might play a role in interaction with this sub- strate of giant size compared to the enzyme. The adsorp- tion to barley star...

Ngày tải lên: 23/03/2014, 07:20

... studies have shown that the binding of FXIIa to the ECM could be due to binding of FXIIa to FN generated during growth of HUVECs [5], we here report on studies of the interactions of FXIIa with FN using ... Fib-2- binding site. The binding site in FXIIa is unknown, but lack of inhibition of the binding of FXIIa to FN by sulfatides and the surfac...

Ngày tải lên: 30/03/2014, 02:20

... at the basal position within the Pterygota. The hemocy- anins from Zygentoma (TdoHc1+ LsaHc1) form the sistergroup of the pterygote proteins. ScuHc1+ FcaHc1 (Collembola) is basal to the ectognathan subunits, ... hemocyanin in the stonefly Perla marginata (Plecoptera) and in the firebrat Thermobia domestica (Zygentoma). We used RT-PCR and RACE experiments to study the pre...

Ngày tải lên: 18/02/2014, 08:20