Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase docx
Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase docx
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Substrate positioning by His92 is important in catalysis
by purple acid phosphatase
Enrico G. Funhoff
1,4
, Yunling Wang
2
, Goran Andersson
2
and Bruce A. Averill
1,3
1 Swammerdam Institute ... imidazole group of His92.
The large (15- to 30-fold) increase in K
M
observed
for His92Ala-recRPAP suggests that this residue is
involved in substrate binding i...
Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... I (NF-I) required to facilitate
initiation of replication in vivo but not in vitro, perhaps by
alleviating nucleosome repression or by assisting in origin
unwinding under conditions of altered ... findings that JCV DNA replication is
stimulated by NF-I in vivo but not in vitro support this
explanation [50]. This view is consistent with the knowledge
that introducing an SV40...
Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot
... for
immunoblotting which were quantified using the bicinchoni-
nic acid protein assay.
Statistical analysis
Data were analysed by one-way ANOVA followed by
Tukey’s test (single time point) or two-way ... Libby P (2007) In ammatory mechanisms: the
molecular basis of in ammation and disease. Nutr Rev
65, S140–S146.
2 Libby P (2002) In ammation in atherosclerosis. Nature
420, 868–874....
Tài liệu Báo cáo khoa học: "The Impact of Query Refinement in the Web People Search Task" docx
... datasets for
this clustering task, with over 15 research groups
submitting results in each campaign. Since the re-
lease of the first datasets, this task is becoming an
increasingly popular research ... search
(WePS) a challenging task, because every single
name is usually shared by many different peo-
ple. One of the mainstream approaches to solve
this problem is designing meta-sear...
Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx
... domains contain fewer arginine and lysine
residues than A domains of other archaeal FtsY proteins. The amino
acid composition of the first 46 positions of archaeal FtsY proteins are
shown. Arginine ... contains 14 positively charged
residues within the first 46 positions, while Pyrococcus
furiosus FtsY contains 12 arginine and lysine residues in this
portion of the protein. In contrast, e...
Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... protein was
examined in an in vitro nPKC kinase assay, using as the substrate
biotin-PKCh pseudosubstrate peptide, which is a rather selective
substrate for PKCh and PKCd.
PKCh in TCDD-induced ... apoptosis is
induced by TCDD, and the failure of actinomycin D
(an inhibitor of gene transcription) and cycloheximide
(an inhibitor of de novo protein synthesis) to block this
apopto...
Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt
... )125 in the 5¢-flanking region containing a
consensus binding site for myocyte enhancer factor 2 (MEF2), to which
binding of MEF2 was augmented by PGF
2a
. Gene silencing of MEF2B
by RNA interference ... expression of MEF2B in VSMC is governed by
ATF-1 itself.
Discussion
The major lines of evidence provided by this study are
as follows: (a) the 5¢-terminus of the rat NOX1 mRNA
ex...
Báo cáo khoa học: Voltage- and Ca2+-activated potassium channels in Ca2+ store control Ca2+ release docx
... 7.3 by add-
ing KOH, and 10 lm ionomycin.
Electroporation of retinal cells for introducing
iberiotoxin
An E3 retina was positioned between a pair of platinum
electrodes separated by 0.6 mm in ... E3 chick retina with the DiOC
5
(3)
staining [31]. In the present study, we studied the chan-
ges in the DiOC
5
(3) fluorescence intensity in the E3
chick retina to gain an insight into th...
Báo cáo khoa học: SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc pptx
... [15]. Histidine-rich repeats are
considered to be potential metal-binding domains
[17,20,21]. Although another zinc transporting domain,
HEXPHE, has also been found, the first histidine in
the ... 66th amino acid in
XM046677 (39th in D31887). The result of 5¢-RACE
indicated that cDNA no longer existed in front of the
first methionine of mouse SLC39A14. Additionally, in
the amino ac...
Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx
... Lig
Tk
.
The ability to discriminate mismatched ends has been
investigated for DNA ligases from several organisms using
synthetic duplex DNA substrates containing 3¢ or 5¢ mis-
matches at their nicks ... single-
strand break in double-stranded DNA [5,6]. They are
essential enzymes for maintaining the integrity of the
genome during DNA replication [7], DNA excision rep air
[8] a nd DNA recom...
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