Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... Journal 272 (2005) 2705–2716 ª 2005 FEBS Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays Costel C. Darie 1 , Martin L. Biniossek 2 , ... to seven out of the 11 genes have been identified in chloroplasts. Four of them are components of the connecting subcomplex: NdhJ [6], NdhH [10...

Ngày tải lên: 23/03/2014, 13:20

... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode o...

Ngày tải lên: 18/02/2014, 08:20

... comprising the two sugar binding pockets in the pulchellins (Fig. 3). In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-...

Ngày tải lên: 18/02/2014, 16:20

... Biotechnology Company. All the other chemi- cals and reagents were of analytical grade. Isolation and purification of lamjapin from kelp The fresh tender leaves of kelp were freeze-dried and powdered in ... were dried and exposed to X-ray film. Assay of the release of adenine from the SRD RNA by lamjapin The preparation of the oligoribonucletides was as des...

Ngày tải lên: 08/03/2014, 10:20

... distributed throughout the plant kingdom and are active against ribosomes from different species, although the level of activity depends on the source of the RIP and of the ribosome. There are many reports ... collected from another region of Crete, and this residue substitution was confirmed. There are no N-glycosylation sites in the deduced sequence. Quality of...

Ngày tải lên: 16/03/2014, 14:20

... samples, and were used for the quantification of the enzymatically formed H 2 S. Measurement of pyruvate formation The activity of the purified d-CDes enzyme was followed through the measurement of the ... to the presence of d-cysteine in the culture medium. It was suggested that d-cysteine exerts its toxicity through an inhibition of threonine deaminase. On the...

Ngày tải lên: 16/03/2014, 18:20

... from the poly(A) of GT-cDNA1 and another is located 11 bp upstream from the poly(A) of GT-cDNA2 (data not shown). Of particular interest is the presence of eight AUUUA motifs in the 3¢-UTR of ... about the hypoxia responsiveness of these genes. Here, we describe the cloning and genomic structure of a hypoxia-responsive glucose transporter gene, gcGLUT from...

Ngày tải lên: 17/03/2014, 03:20

... detailed analysis of the 12F-11 paratope and assessment of the varying contributions of CDR and framework regions clearly requires definitive structural data; this analysis is currently in progress. The isolation ... Analysis of the binding of the Fab fragment of monoclonal antibody NC10 to influenza virus N9 neuraminidase from tern and whale using the BIAcor...

Ngày tải lên: 17/03/2014, 10:20

... attributable to this enzyme. On the other hand, the solution of FerB was yellowish in color and exhibited absorption bands at 380 and 448 nm (Fig. 3), diagnostic for the presence of a flavin group. When ... (in kDa) on the right. Fig. 2. N-Terminal amino acid sequences of P. denitrificans FerA and FerB (A) and comparison of the N-terminal amino acid sequence of FerB...

Ngày tải lên: 30/03/2014, 13:20

... combinations of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails. A ... patterns Fig. 1. Structural organization of the XHIVEP proteins. Black bars indicate the position of the ZAS C 2 H 2 zinc fingers (ZAS-N and...

Ngày tải lên: 30/03/2014, 13:20