Báo cáo khoa học: Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis pot

... differences of HPrSerP and CrhP FEBS Journal 272 (2005) 2566–2577 ª 2005 FEBS 2577 Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis Gerald ... couple aminomodified DNA to that chip and measured CcpA cre binding, HPrSerP stimu- lation of CcpA cre binding and their reaction rates. Initial expe...

Ngày tải lên: 23/03/2014, 13:20

... proteolysis of extracellular matrix. Most MMPs are secreted as inactive proenzymes and latency is maintained by an interaction between a cystein residue in the prodomain and Zn 2+ in the active site of ... FEBS protein in the two cell lines was determined as described in Materials and methods, and 81 lg of pro- tein was added to each well. The amount of S100A4 in the...

Ngày tải lên: 18/02/2014, 11:20

... turnover in marine and soil ecosystems. In the sea, chitin is found as the main compound of the exoskeleton of crustaceans, and on land it is an essential structural component of insects and the ... fungi contain on average 15–20 genes encoding chitinases, but only two or three genes encoding GH family 20 proteins. Potential functions of chitin-degrading enzymes in fungi i...

Ngày tải lên: 23/03/2014, 05:22

... divided into three groups, a-helical (e.g. cecropin and magainin), b-sheet or b-hairpin, stabilized by disulfide bonds (e.g. defensins, tachyplesins, and protegrins), and extended (e.g. indo- licidin ... determined the binding sites of LPS in the presence of polymyxin antibiotic peptides, using DPC-solublized isotope-labeled LPS [66]. Remaining challenges In order to form pores...

Ngày tải lên: 29/03/2014, 22:21

... visibility of the AMI Corpus. 1 Introduction The AMI Meeting Corpus (Carletta and al., 2006) is one of the largest and most extensively annotated data sets of multimodal recordings of human interac- tion. ... particular coding schemes. This paper describes work on the generation of annotation and metadata databases in order to in- crease the usability of these components...

Ngày tải lên: 08/03/2014, 02:21

... train on a quarter of the training set and classify words in sequence. We only use a portion of the training data to in- crease the percentage of words unseen in training. We also compare to using ... that training single classifiers and combining their outcomes almost always outperforms a single joint classi- fier for the three target features. In other words, combining the r...

Ngày tải lên: 24/03/2014, 03:20

... Morphology Somali has a fairly standard set of in ectional af- fixes for nouns and verbs, as outlined below. In addition, there are a substantial set of ‘spelling rules’ which insert and delete graphemes ... processes of applying spelling rules and inspecting the lexicon are appropriately interwoven. The definite articles, in particular, require considerable care. There are a n...

Ngày tải lên: 31/03/2014, 01:20

... binding of a DCMU molecule in the Q B pocket; the remaining PSII centers are unaffected. However, a saturating concen- tration of DCMU would inhibit completely the activity of PSII by preventing ... Q A to Q B [21,22], is of importance for modulating the dynamics of PQ pool reduction and determining its effect on FI kinetics as discussed below. The increase in A O–J observed...

Ngày tải lên: 19/02/2014, 05:20

... function of pH and temperature In order to determine the effect of pH and temperature on the cyclization of the N-terminal glutaminyl to pyrogluta- myl, 0.1 m M (Met1)-ONC (M23L), in 0.2 M potassium phosphate ... almost linear and can be obtained by calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredt...

Ngày tải lên: 19/02/2014, 12:20

... channeling Prior investigations of channeling may be divided into two categories: in vitro and in vivo. The former include experiments with cell extracts and purified enzymes. These are inherently unsatisfying. ... [ 14 C]aminoacyl-tRNA (made in the pathway from 14 C-labeled amino acids), [ 3 H]aminoacyl-tRNA, 14 C- and 3 H-labeled protein were measured. The 3 Hin protein was ins...

Ngày tải lên: 19/02/2014, 18:20