Báo cáo khoa học: Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation of bacteriorhodopsin 15 docx

... O-states of BR seen by solid-state NMR A. J. Mason et al. 2164 FEBS Journal 272 (2005) 2152 –2164 ª 2005 FEBS Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation ... a proton is donated from the Schiff base to Asp85 and another proton is released to the extracellular surface, and the second is the N-N¢-O-bR sequence, whe...

Ngày tải lên: 23/03/2014, 13:20

... representation of one coil of sbwAFP (red, residues G ly34 to T hr49) and TmAFP (blue, residues Asn29fiGly41). Letters de note the five residues of on e of the three sides of s bwAFP o r s ix residues of on ... [53]. Role of the TXT motif and water in activity Examination of the crystal structures of the insect AFPs also revealed the presence of an array...

Ngày tải lên: 19/02/2014, 16:20

... capable of protecting the fish from freezing below the freezing point of seawater calls into question the functional significance of the small type I AFP in these species. Considering the levels of these ... AFPs in the plasma it seems unlikely that they are excess to the needs of the fish. In addition, the abundance of the skin-type AFP in epithelial tissues also...

Ngày tải lên: 20/02/2014, 01:20

... formation, which leads to the movement of the charged groups of the protein in order to keep themselves neutral. As a means of testing the role of the surfactant on the protein conformations, ... content. The radius of the volume element [R i ¼ (3V i / 4pN a ) 1/3 ] gives a measure of the proximity of the quencher molecule to the fluorophore. The calculate...

Ngày tải lên: 07/03/2014, 15:20

... by the viscosity of the medium. The fundamental anisotropy, r o , representing the total anisotropy in the absence of rotation (at t ¼ 0), is equal to the sum of the amplitudes, b i , of the ... integrity of the external surface of LamA upon thermal and chemical treatment were tes- ted by measuring the exposure of the hydrophobic groups to the solvent. T...

Ngày tải lên: 07/03/2014, 21:20

... immediately after the transition temperature. The degree of reversibility decreased with the temperature to which the sample of 17b-HSDcl was heated (data not shown). The thermodynamic profile of 17b-HSDcl ... affected by ther- modynamic coupling to the acid ⁄ base equilibrium of the acidic amino acid residues. Electrophoretic titration analysis The results of e...

Ngày tải lên: 16/03/2014, 13:20

... from the Trp residue, buried in the interior of the protein molecule, to the ionone ring of retinol would be expected. Inter- estingly, binding of retinol caused an increase of the emission of the ... cells of the inner hypodermis and the epithe- lium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms. Discussion A bundle...

Ngày tải lên: 16/03/2014, 18:20

... that of DnaK. The twofold reduction of the K SV values estimated for mtHsp70 both in the absence and the presence of ATP (Table 2) supports the existence of differences in the Trp environment of ... that the lid did not close tightly the binding site of these chimeras. Thus, this finding indicates that the interaction of mtHsp70 PBD with the DnaK ATPase domain...

Ngày tải lên: 16/03/2014, 22:20

... located on the top of the substrate-binding site in the nuclease domain of the other subunit, which implies that it may participate in the binding with the poly(A) substrate [14]. In the primary ... increase occurred in the size of the protein. Meanwhile, the peak area of the eluted proteins in the SEC profile was greatly reduced compared with that of the na...

Ngày tải lên: 23/03/2014, 04:21

... processes of the apo form of the protein (apoNgb) as a result of the formation of an inter- molecular disulfide bond. The preparation of apoNgb was obtained from the corresponding holoprotein by the removal ... on the apo and holo forms of the two proteins reveal the persistence of helix F in neu- roglobin even in the absence of heme. Conversely myoglobin sh...

Ngày tải lên: 30/03/2014, 01:20