Báo cáo khoa học: Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts pot

... 2021 Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts Jason L. Parsons, Irina I. Dianova, Sarah L. Allinson* and Grigory ... than interaction of BER proteins with PARP-1. PARP-1 binding protects repair intermediates from deterioration It was previously proposed that PARP-1 binding m...

Ngày tải lên: 23/03/2014, 13:20

... phosphatidylinositol-3-kinase, as well as activating tyrosine phosphorylation. An increase in intracellular levels of cAMP [6,7] and the involvement of kinases, such as protein kinase C (PKC) ... cross-linking proteins, components of the actin filaments and microtubules, as well as with b-integrin vesicle trafficking [27] and therefore the link between PKC b and b 2 integrins may not be coinc...

Ngày tải lên: 07/03/2014, 16:20

... Poly- pyrimidine tract-binding protein promotes insulin secretory granule biogenesis. Nat Cell Biol 6, 207–214. 10 Kosinski PA, Laughlin J, Singh K & Covey LR (2003) A complex containing polypyrimidine ... ) ES cells. (A) Expression of cell cycle- related proteins in Ptb ) ⁄ ) ES cells. Expres- sion of p27, cyclin A, cyclin B and cyclin E was examined by western blotting. Whole cel...

Ngày tải lên: 07/03/2014, 00:20

... Bourgoin, S., Jean, N., McColl, S.R. & Nac- cache, P.H. (1995) Diverging signal transduction pathways acti- vated by interleukin-8 and related chemokines in human neutrophils: interleukin-8, ... Bucks., UK). Human recombinant TNFa was supplied by R & D Systems (Abingdon, Oxon, UK). Wortmannin, brefeldin A and propranolol were obtained from Calbiochem (Nottingham, UK). Fetal bo...

Ngày tải lên: 07/03/2014, 15:20

... train- ing corpus. Training, tuning, and decoding were performed using the Moses toolkit 3 . Tuning (learning the λ values discussed in section 4.1) was done using minimum error rate training ... morphological, and other lin- guistic information in a log-linear model. This work also describes experiments in translating from English to German, Spanish, and Czech, in- cluding the use...

Ngày tải lên: 08/03/2014, 00:20

... Ca 2+ in ux. Effect of EPCG, catechin hydrate, curcumin, apige- nin, naringenin and gallotannin on QUIN-induced intracellular Ca 2+ in cultured human neurons As mentioned above, QUIN stimulation induced ... correlated to DNA strand breaks [11]. Results Effect of EPCG, catechin hydrate, curcumin, apigenin, naringenin and gallotannin on QUIN-induced nNOS activity and extracellular ni...

Ngày tải lên: 22/03/2014, 21:20

... Science, Allergan, Inc., Irvine, CA, USA Heterotrimeric guanine nucleotide-binding proteins (G proteins) comprise a superfamily that is involved in the transmission of ligand binding to cell surface ... Corp., Inc., Indianapolis, IN, USA), according to manufacturer’s instructions. In brief, cells were washed twice and resuspended in 1 mL of DMEM. One microgram of plasmid DNA in 1...

Ngày tải lên: 23/03/2014, 13:20

... mechanism of DNA- induced stabilization of the protein, and whe- ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased light scattering of ... conserved arginine-rich RNA binding motif in cowpea chlorotic mottle virus capsid protein results in virion structural alterations and RNA packa- ging constraints. J Virol 79, 3277–3288....

Ngày tải lên: 30/03/2014, 11:20

... digested in proteinase K (20 lgÆmL )1 ) for 20 min, rinsed in NaCl ⁄ Tris, and then fixed in 4% PFA for 10 min. Sections were then washed in 0.2% glycine, rinsed and incubated for 30 min in acetylation ... antibody. Finally, cells were stained with 4¢,6-diamidino-2-phenylindole diluted 1 : 7500 in NaCl ⁄ P i for 10 min to visualize nuclei. LCM Brain coronal cryosections (10 lm) obt...

Ngày tải lên: 29/03/2014, 21:20

... In addition, Phe427 in rMaL (Val428 in TaLcc1) might be involved in placing substrate molecules into the cor- rect orientation for oxidation. In T. hirsuta, the loops forming the substrate-binding ... dimeric interface in TaLcc1 (B) and rMaL (C). The residues from molecule A are shown in purple, and those from molecule B in yellow. The hydrogen bonding residues (according...

Ngày tải lên: 14/02/2014, 18:20