Báo cáo khoa học: Small exterior hydrophobic cluster contrib

Báo cáo khoa học: Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B pot

... contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B Young S. Yun 1,2 , Gyu H. Nam 1,2 , Yeon-Gil Kim 1,3 , Byung-Ha Oh 1,3 and ... solvent-exposed b- strands and helps to bind hydrophobic steroids....

Ngày tải lên : 23/03/2014, 13:20

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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... inhibitor baﬁlomycin and the cathepsin D inhibitor pepstatin A partially inhibited, both in vivo and in vitro, the cAMP response to cholera toxin. This cathepsin D-dependent action of cholera toxin ... [2]. CT belongs to the AB family of bacterial exotoxins, and consists of a pentameric B subunit (CT -B) and an A subunit (CT-A) compri...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... be used as a substrate for testing AUH activity in a routine setting. Indeed, we were able to show that AUH activity in ﬁbro- blasts can be determined by monitoring the forma- tion of 3-hydroxyglutaryl-CoA. ... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU binding homolog of enoyl-CoA hydratase’). The RNA- bindi...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... chemicals including amino acids, nucleic bases and vitamins were obtained from commercial sources. Acetylpyridine adenine dinucleotide (APAD) and 6-diazo-5-oxo- L -norleucine (DON) were from Sigma ... dehydrogenase from bovine liver was obtained from Oriental Yeast (Tokyo, Japan). Reagents for genetic engineering such as restriction enzymes were purc...

Ngày tải lên : 19/02/2014, 12:20

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Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt

... (2006) Tachykinins and tachykinin receptors in the gut, with special reference to NK2 receptors in human. Auton Neurosci 126–127, 232–249. 32 Burnstock G (2008) The journey to establish purinergic signalling ... release of ATP in the bladder by in ux via CD38 and subse- quent activation of intracellular cADPR receptors, probably causing a...

Ngày tải lên : 05/03/2014, 23:20

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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx

... continuous tempera- ture recording in the incubator. A twin incubator with an inactive antenna was used for sham exposure. Samples were coded before transfer from the standard culture incubator to ... medium and the cellular models were put in the culture incubator to recover for up to 24 (hRE) or 48 h (hRE and cells). UVB source UVB ir...

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx

... similarity to other known cysteine-containing chitin -binding domains [14], such as plant chitin -binding proteins and horseshoe crab antimicrobial peptides with chitin -binding afﬁnity [15–17]. Rebers and ... C-terminal domain of tachycitin (Cys40 to Cys61), a horseshoe crab chitin -binding protein [15], as well as with the chitin -bin...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf

... research interest in the past, A B Fig. 9. Structure of RMD from A. thermoaerophilus. (A) Stereoview of the RMD monomer. The cofactor -binding domain and the substrate- binding domain are shown in aqua ... sugar nucleotide- modifying SDR family, folding into two domains: a Rossmann fold domain, which binds cofactor, and a mixed a /b domain,...

Ngày tải lên : 16/03/2014, 01:20

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Báo cáo khoa học: The effect of heme on the conformational stability of micro-myoglobin doc

... 2007) doi:10.1111/j.1742-4658.2007.06176.x Micro-myoglobin, the isolated heme -binding subdomain of myoglobin, is a valuable model system for the investigation of heme recognition and binding by proteins, and provides an example of protein folding ... heme binding can be segregated from those for protein stability and solubility [9]. Thus, lM...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

... was from Biolabs. b- Galactosidase and b- hexosaminidase (both isolated from Jack bean) were from Oxford GlycoSystems (Abingdon, Oxon, UK). Glucosidase II puriﬁed from rat liver was a gift from ... of oligomannosides bound to glycoproteins after 20 min of preincubation at 0.175 m M Glc followed by 1 h of labeling and a 1 h chase in <...

Ngày tải lên : 16/03/2014, 16:20

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