Báo cáo khoa học: Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B pot

... contributes < /b> to < /b> conformational < /b> stability < /b> and < /b> steroid < /b> binding < /b> in < /b> ketosteroid < /b> isomerase < /b> from < /b> Pseudomonas < /b> putida < /b> biotype < /b> B Young S. Yun 1,2 , Gyu H. Nam 1,2 , Yeon-Gil Kim 1,3 , Byung-Ha Oh 1,3 and < /b> ... solvent-exposed b- strands and < /b> helps to < /b> bind hydrophobic < /b> steroids....

Ngày tải lên: 23/03/2014, 13:20

... inhibitor bafilomycin and < /b> the cathepsin D inhibitor pepstatin A partially inhibited, both in < /b> vivo and < /b> in < /b> vitro, the cAMP response to < /b> cholera toxin. This cathepsin D-dependent action of cholera toxin ... [2]. CT belongs to < /b> the AB family of bacterial exotoxins, and < /b> consists of a pentameric B subunit (CT -B) and < /b> an A subunit (CT-A) compri...

Ngày tải lên: 19/02/2014, 02:20

... be used as a substrate for testing AUH activity in < /b> a routine setting. Indeed, we were able to < /b> show that AUH activity in < /b> fibro- blasts can be determined by monitoring the forma- tion of 3-hydroxyglutaryl-CoA. ... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- bindi...

Ngày tải lên: 19/02/2014, 07:20

... chemicals including amino acids, nucleic bases and < /b> vitamins were obtained from < /b> commercial sources. Acetylpyridine adenine dinucleotide (APAD) and < /b> 6-diazo-5-oxo- L -norleucine (DON) were from < /b> Sigma ... dehydrogenase from < /b> bovine liver was obtained from < /b> Oriental Yeast (Tokyo, Japan). Reagents for genetic engineering such as restriction enzymes were purc...

Ngày tải lên: 19/02/2014, 12:20

... (2006) Tachykinins and < /b> tachykinin receptors in < /b> the gut, with special reference to < /b> NK2 receptors in < /b> human. Auton Neurosci 126–127, 232–249. 32 Burnstock G (2008) The journey to < /b> establish purinergic signalling ... release of ATP in < /b> the bladder by in< /b> ux via CD38 and < /b> subse- quent activation of intracellular cADPR receptors, probably causing a...

Ngày tải lên: 05/03/2014, 23:20

... continuous tempera- ture recording in < /b> the incubator. A twin incubator with an inactive antenna was used for sham exposure. Samples were coded before transfer from < /b> the standard culture incubator to < /b> ... medium and < /b> the cellular models were put in < /b> the culture incubator to < /b> recover for up to < /b> 24 (hRE) or 48 h (hRE and < /b> cells). UVB source UVB ir...

Ngày tải lên: 07/03/2014, 11:20

... similarity to < /b> other known cysteine-containing chitin -binding < /b> domains [14], such as plant chitin -binding < /b> proteins and < /b> horseshoe crab antimicrobial peptides with chitin -binding < /b> affinity [15–17]. Rebers and < /b> ... C-terminal domain of tachycitin (Cys40 to < /b> Cys61), a horseshoe crab chitin -binding < /b> protein [15], as well as with the chitin -bin...

Ngày tải lên: 07/03/2014, 21:20

... research interest in < /b> the past, A B Fig. 9. Structure of RMD from < /b> A. thermoaerophilus. (A) Stereoview of the RMD monomer. The cofactor -binding < /b> domain and < /b> the substrate- binding < /b> domain are shown in < /b> aqua ... sugar nucleotide- modifying SDR family, folding into two domains: a Rossmann fold domain, which binds cofactor, and < /b> a mixed a /b domain,...

Ngày tải lên: 16/03/2014, 01:20

... 2007) doi:10.1111/j.1742-4658.2007.06176.x Micro-myoglobin, the isolated heme -binding < /b> subdomain of myoglobin, is a valuable model system for the investigation of heme recognition and < /b> bind- ing by proteins, and < /b> provides an example of protein folding ... heme binding < /b> can be segregated from < /b> those for protein stability < /b> and < /b> solubility [9]. Thus, lM...

Ngày tải lên: 16/03/2014, 06:20

... was from < /b> Biolabs. b- Galactosidase and < /b> b- hexosaminidase (both isolated from < /b> Jack bean) were from < /b> Oxford GlycoSystems (Abingdon, Oxon, UK). Glucosidase II purified from < /b> rat liver was a gift from < /b> ... of oligomannosides bound to < /b> glycoproteins after 20 min of preincubation at 0.175 m M Glc followed by 1 h of labeling and < /b> a 1 h chase in <...

Ngày tải lên: 16/03/2014, 16:20